We are preparing the requested document. Please wait, this may take a while...!
|Antigen||Protein Kinase C, alpha (PKCa) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This PKC alpha antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|2 references available|
|Supplier||Log in to see|
Product Details anti-PKC alpha AntibodyTarget Details PKC alpha Application Details Handling References for anti-PKC alpha antibody (ABIN453789) Images
|Specificity||This antibody reacts to PRKCA.|
|Purification||Saturated Ammonium Sulfate (SAS) precipitation|
|Immunogen||KLH conjugated synthetic peptide selected from the N-terminal region of human PRKCA|
Target Details PKC alphaProduct Details anti-PKC alpha Antibody Application Details Handling References for anti-PKC alpha antibody (ABIN453789) Images back to top
|Alternative Name||PRKCA (PKCa Antibody Abstract)|
|Background||PRKCA is a calcium-activated, phospholipid-dependent, serine-and threonine-specific enzyme. It may play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.Synonyms: PKC alpha, PKC-A, PKC-alpha, PKCA, PRKACA, Protein kinase C alpha type|
|Pathways||WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling|
Application DetailsProduct Details anti-PKC alpha Antibody Target Details PKC alpha Handling References for anti-PKC alpha antibody (ABIN453789) Images back to top
ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PKC alpha Antibody Target Details PKC alpha Application Details References for anti-PKC alpha antibody (ABIN453789) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-PKC alpha antibody (ABIN453789)Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling Images back to top
Gysin, Imber: "Replacement of Ser657 of protein kinase C-alpha by alanine leads to premature down regulation after phorbol-ester-induced translocation to the membrane." in: European journal of biochemistry / FEBS, Vol. 240, Issue 3, pp. 747-50, 1996
Rider, Vandamme, Lebeau, Vertommen, Vidal, Rousseau, Vandekerckhove, Hue: "The two forms of bovine heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase result from alternative splicing." in: The Biochemical journal, Vol. 285 ( Pt 2), pp. 405-11, 1992
ImagesProduct Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling References for anti-PKC alpha antibody (ABIN453789) back to top