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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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1 | ABIN4345802 | ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA WB | Mouse | IgG2a | Log in to see | MC5 | 0 | |||
1 | ABIN4345822 | ELISA FACS ICC IF IHC (fro) IHC (p) WB | Alexa Fluor 488 | Mouse | IgG2a | Log in to see | MC5 | 0 | ||
1 | ABIN4345824 | ELISA FACS ICC IF IHC (fro) IHC (p) WB | Alexa Fluor 700 | Mouse | IgG2a | Log in to see | MC5 | 0 | ||
1 | ABIN4345819 | ELISA FACS ICC IF IHC (fro) IHC (p) WB | DyLight 488 | Mouse | IgG2a | Log in to see | MC5 | 0 | ||
1 | ABIN4345821 | ELISA FACS ICC IF IHC (fro) IHC (p) WB | Alexa Fluor 405 | Mouse | IgG2a | Log in to see | MC5 | 0 | ||
1 | ABIN4345811 | ELISA FACS ICC IF IHC (fro) IHC (p) | FITC | Mouse | IgG2a | Log in to see | MC5 | 0 | ||
1 | ABIN4345815 | ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA WB | PerCP | Mouse | IgG2a | Log in to see | MC5 | 0 | ||
1 | ABIN4345817 | ELISA FACS ICC IF IHC (fro) IHC (p) WB | DyLight 755 | Mouse | IgG2a | Log in to see | MC5 | 0 | ||
1 | ABIN4345823 | ELISA FACS ICC IF IHC (fro) IHC (p) WB | Alexa Fluor 647 | Mouse | IgG2a | Log in to see | MC5 | 0 | ||
1 | ABIN4345813 | ELISA FACS ICC IF IHC (fro) IHC (p) WB | DyLight 405 | Mouse | IgG2a | Log in to see | MC5 | 0 | ||
1 | ABIN4345814 | ELISA FACS ICC IF IHC (fro) IHC (p) WB | DyLight 350 | Mouse | IgG2a | Log in to see | MC5 | 0 | ||
1 | ABIN4345818 | ELISA FACS ICC IF IHC (fro) IHC (p) WB | DyLight 680 | Mouse | IgG2a | Log in to see | MC5 | 0 | ||
1 | ABIN5599620 | ELISA ICC IF IHC IHC (fro) IHC (p) WB | Biotin | Mouse | IgG2a | Log in to see | MC5 | 0 | ||
1 | ABIN5599622 | ELISA FACS ICC IF IHC IHC (fro) IHC (p) WB | DyLight 650 | Mouse | IgG2a | Log in to see | MC5 | 0 | ||
1 | ABIN5599621 | ELISA FACS ICC IF IHC IHC (fro) IHC (p) WB | DyLight 550 | Mouse | IgG2a | Log in to see | MC5 | 0 | ||
1 | ABIN5599623 | ELISA IHC IHC (fro) IHC (p) WB | HRP | Mouse | IgG2a | Log in to see | MC5 | 0 |
General |
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Antigen | Protein Kinase C, alpha (PKCa) Antibodies |
Reactivity | Cow (Bovine), Dog (Canine), Fish, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine) Alternatives |
Host | Mouse Alternatives |
Clonality (Clone) | |
Conjugate | This PKC alpha antibody is un-conjugated Alternatives |
Application |
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
Alternatives
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14 references available |
Supplier | Log in to see |
Product Details anti-PKC alpha AntibodyTarget Details PKC alpha Application Details Handling References for anti-PKC alpha antibody (ABIN153146) Images |
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Specificity | This antibody is capable of detecting PKC beta as well. |
Purification | Protein A purified |
Immunogen | Purified bovine brain Protein Kinase C |
Clone | MC5 |
Isotype | IgG2a |
Target Details PKC alphaProduct Details anti-PKC alpha Antibody Application Details Handling References for anti-PKC alpha antibody (ABIN153146) Images back to top |
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Antigen | |
Alternative Name | PKC alpha (PKCa Antibody Abstract) |
Background | Gene Symbol: PRKCA |
Gene ID | 5578 |
UniProt | P17252, P04409 |
Pathways | WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling |
Application DetailsProduct Details anti-PKC alpha Antibody Target Details PKC alpha Handling References for anti-PKC alpha antibody (ABIN153146) Images back to top |
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Application Notes | Western Blot 1:1000, Flow Cytometry 1:10-1:1000, ELISA 1:100-1:2000, Immunohistochemistry 1:200-1:500, Immunocytochemistry/Immunofluorescence 1:100-1:500, Immunoprecipitation 1:10-1:100, Immunohistochemistry-Paraffin 1:200-1:500, Immunohistochemistry-Frozen 1:200-1:500, RadioimmunoassayThis PKC alpha Antibody (MC5) is useful for Western blot, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation and Radioimmunoassay. In WB a band can be seen at ~75 kDa representing PKC alpha. |
Comment |
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo. |
Restrictions | For Research Use only |
HandlingProduct Details anti-PKC alpha Antibody Target Details PKC alpha Application Details References for anti-PKC alpha antibody (ABIN153146) Images back to top |
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Format | Liquid |
Concentration | 1.0 mg/mL |
Buffer |
Tris-Glycine and 0.15M NaCl Buffer contains: 0.05 % Sodium Azide |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handling Advice | Avoid freeze-thaw cycles |
Storage | 4 °C,-20 °C |
Storage Comment | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
References for anti-PKC alpha antibody (ABIN153146)Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling Images back to top |
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Product cited in: |
Xie, Huo, Li, Dai, Xu, Yin: "Olfactory Ensheathing Cells Inhibit Gliosis in Retinal Degeneration by Downregulation of the Müller Cell Notch Signaling Pathway." in: Cell transplantation, Vol. 26, Issue 6, pp. 967-982, 2018 Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Rat (Rattus)). Guimarães-Souza, Perche, Morgans, Duvoisin, Calaza: "Fragile X Mental Retardation Protein expression in the retina is regulated by light." in: Experimental eye research, Vol. 146, pp. 72-82, 2016 Zimov, Yazulla: "Novel processes invaginate the pre-synaptic terminal of retinal bipolar cells." in: Cell and tissue research, Vol. 333, Issue 1, pp. 1-16, 2008 Quraishi, Gayet, Morgans, Duvoisin: "Distribution of group-III metabotropic glutamate receptors in the retina." in: The Journal of comparative neurology, Vol. 501, Issue 6, pp. 931-43, 2007 Bayley, Morgans: "Rod bipolar cells and horizontal cells form displaced synaptic contacts with rods in the outer nuclear layer of the nob2 retina." in: The Journal of comparative neurology, Vol. 500, Issue 2, pp. 286-98, 2006 Ng, Parsons, Hughes, Monypenny, Zicha, Gautreau, Arpin, Gschmeissner, Verveer, Bastiaens, Parker: "Ezrin is a downstream effector of trafficking PKC-integrin complexes involved in the control of cell motility." in: The EMBO journal, Vol. 20, Issue 11, pp. 2723-41, 2001 (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunoprecipitation,Immunofluorescence Cesare, Dekker, Sardini, Parker, McNaughton: "Specific involvement of PKC-epsilon in sensitization of the neuronal response to painful heat." in: Neuron, Vol. 23, Issue 3, pp. 617-24, 1999 (Sample species: Rat (Rattus)). Further details: Western Blotting Ricciarelli, Tasinato, Clément, Ozer, Boscoboinik, Azzi: "alpha-Tocopherol specifically inactivates cellular protein kinase C alpha by changing its phosphorylation state." in: The Biochemical journal, Vol. 334 ( Pt 1), pp. 243-9, 1998 (Sample species: Rat (Rattus)). Further details: Western Blotting Gatti, Robinson: "Unique phosphorylation of protein kinase C-alpha in PC12 cells induces resistance to translocation and down-regulation." in: The Journal of biological chemistry, Vol. 271, Issue 49, pp. 31718-22, 1997 (Sample species: Rat (Rattus)). Further details: Western Blotting Clément, Tasinato, Boscoboinik, Azzi: "The effect of alpha-tocopherol on the synthesis, phosphorylation and activity of protein kinase C in smooth muscle cells after phorbol 12-myristate 13-acetate down-regulation." in: European journal of biochemistry, Vol. 246, Issue 3, pp. 745-9, 1997 (Sample species: Rat (Rattus)). Further details: Western Blotting Gatti, Robinson: "Okadaic acid interferes with phorbol-ester-mediated down-regulation of protein kinase C-alpha, C-delta and C-epsilon." in: European journal of biochemistry, Vol. 249, Issue 1, pp. 92-7, 1997 (Sample species: Rat (Rattus)). Further details: Western Blotting Wyss-Coray, Masliah, Toggas, Rockenstein, Brooker, Lee, Mucke: "Dysregulation of signal transduction pathways as a potential mechanism of nervous system alterations in HIV-1 gp120 transgenic mice and humans with HIV-1 encephalitis." in: The Journal of clinical investigation, Vol. 97, Issue 3, pp. 789-98, 1996 Further details: Immunocytochemistry,Immunofluorescence Escribá, Sastre, García-Sevilla: "Disruption of cellular signaling pathways by daunomycin through destabilization of nonlamellar membrane structures." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 16, pp. 7595-9, 1995 (Sample species: Rat (Rattus)). Further details: Western Blotting Lacal, Cuadrado, Jones, Trotta, Burstein, Thomson, Pellicer: "Regulation of protein kinase C activity in neuronal differentiation induced by the N-ras oncogene in PC-12 cells." in: Molecular and cellular biology, Vol. 10, Issue 6, pp. 2983-90, 1990 (Sample species: Human). Further details: Western Blotting |
ImagesProduct Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling References for anti-PKC alpha antibody (ABIN153146) back to top |
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