anti-Rat (Rattus) PKC alpha antibody for Flow Cytometry

Recommended PKC alpha Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, alpha (PKCa) Antibodies
  • pkc-alpha
  • pkcalpha
  • im:7139045
  • si:dkey-8l19.1
  • LOC100219195
  • AAG6
  • PKC-alpha
  • PKCA
  • PRKACA
  • AI875142
  • Pkca
  • PKRCA
  • protein kinase C alpha
  • protein kinase C, alpha L homeolog
  • protein kinase C, alpha
  • PRKCA
  • prkca.L
  • prkca
  • Prkca
Epitope
pThr497
36
20
18
17
16
16
15
14
12
9
7
6
5
5
5
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
306
219
175
31
26
25
21
21
20
5
3
3
2
1
1
1
1
Host
Rabbit
274
44
2
Clonality
Monoclonal
Conjugate
This PKC alpha antibody is un-conjugated
14
13
10
7
6
5
5
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Western Blotting (WB)
260
151
136
69
55
50
32
32
26
23
13
5
4
4
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN6390731
$ 676.92
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4904772 FACS ICC IF IHC IP WB Rabbit IgG Log in to see 0
1 ABIN331530 FACS IP IHC WB Mouse IgG2a Log in to see MC5 0
1 ABIN220833 ICC FACS IF IP WB Rabbit Log in to see Polyclonal 0

General

Antigen Protein Kinase C, alpha (PKCa) Antibodies
Epitope pThr497
(36), (20), (18), (17), (16), (16), (15), (14), (12), (9), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(306), (219), (175), (31), (26), (25), (21), (21), (20), (5), (3), (3), (2), (1), (1), (1), (1)
Host Rabbit
(274), (44), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PKC alpha antibody is un-conjugated
(14), (13), (10), (7), (6), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Western Blotting (WB)
(260), (151), (136), (69), (55), (50), (32), (32), (26), (23), (13), (5), (4), (4), (2), (1), (1), (1)
Supplier Log in to see

Product Details anti-PKC alpha Antibody

Target Details PKC alpha Application Details Handling Images
Purification

Purification Method: Protein A+G

Purified From: Cell culture

Immunogen A synthetic phospho-peptide corresponding to residues surrounding Thr497 of human phospho PKCalpha
Clone PKCaT497-F1
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details PKC alpha

Product Details anti-PKC alpha Antibody Application Details Handling Images back to top
Antigen
Alternative Name PKCa (PKCa Antibody Abstract)
Background Protein kinase C (PKC) activation is one of the earliest events in a cascade controlling many cellular responses, including proliferation, gene expression, secretion, and muscle contraction (1,2). The three groups of PKC isoforms are distinuished by their specific activators as well as calcium dependency. The PDK1 enzyme, or a closely related protein, is responsible for PKC activation. PKC also undergoes autophosphorylation.
Pathways WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-PKC alpha Antibody Target Details PKC alpha Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Images back to top
Concentration 0.5 mg/mL
Buffer

Formulation: 1X PBS, 0.02 % Sodium azide, 50 % Glycerol, 0.1 % BSA

 pH : 7.4

Images

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Protein Kinase C, alpha (PKCa) (pThr497) antibody (ABIN6390731) Flow cytometric analysis of K562 cells secondary antibody only negative control (blue...
Western Blotting (WB) image for anti-Protein Kinase C, alpha (PKCa) (pThr497) antibody (ABIN6390731) Western blot analysis of HT1080 cell extract, untreated or treated with TPA using 0.0...
Western Blotting (WB) image for anti-Protein Kinase C, alpha (PKCa) (pThr497) antibody (ABIN6390731) Western blot analysis of HT1080 cell extract untreated or treated with TPA using 0.05...