anti-Rat (Rattus) PKC alpha antibody for Western Blotting

Recommended PKC alpha Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, alpha (PKCa) Antibodies
  • pkc-alpha
  • pkcalpha
  • im:7139045
  • si:dkey-8l19.1
  • LOC100219195
  • AAG6
  • PKC-alpha
  • PKCA
  • PRKACA
  • AI875142
  • Pkca
  • PKRCA
  • protein kinase C alpha
  • protein kinase C, alpha L homeolog
  • protein kinase C, alpha
  • PRKCA
  • prkca.L
  • prkca
  • Prkca
Epitope
C-Term
35
20
18
17
16
16
15
14
12
10
7
6
5
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine), Rat (Rattus), Human
309
219
176
31
26
25
21
21
20
6
3
3
2
1
1
1
1
Host
Rabbit
277
45
2
Clonality
Polyclonal
Conjugate
This PKC alpha antibody is un-conjugated
14
13
10
7
6
5
5
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
264
154
135
71
56
50
34
32
26
24
13
5
4
4
2
1
1
1
Options
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Catalog No. ABIN3020835
$ 393.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
19.806385 ABIN5997084 IF/ICC IHC IP WB Rabbit IgG Log in to see 0
17.063175 ABIN3186466 ELISA IHC WB Rabbit IgG Thr287 Log in to see Polyclonal 0
17.063175 ABIN3182236 ELISA WB Rabbit IgG pTyr657 Log in to see Polyclonal 0
17.063175 ABIN3182587 ELISA IHC WB Rabbit IgG pThr638 Log in to see Polyclonal 0
16.806385 ABIN6269891 ELISA IHC WB Rabbit IgG pTyr365 Log in to see Polyclonal 0
16.25679 ABIN870311 IP ELISA WB Rabbit Log in to see G13-R 0
13.806385 ABIN6255718 ELISA ICC IF IHC WB Rabbit IgG pThr638 Log in to see Polyclonal 0
13.806385 ABIN6269893 ELISA IHC WB Rabbit IgG pTyr195 Log in to see Polyclonal 0
13.806385 ABIN1874290 IHC WB Rabbit IgG Log in to see Polyclonal 0
13.25679 ABIN3186465 ELISA WB Rabbit IgG Thr286 Log in to see Polyclonal 0
13.25679 ABIN2149095 IHC ELISA WB Rabbit IgG pThr638 Log in to see Polyclonal 0
13.25679 ABIN2149096 ELISA WB Rabbit IgG pTyr657 Log in to see Polyclonal 0
13.25679 ABIN1689921 IHC (p) WB Rabbit Log in to see Polyclonal 0
12.306385 ABIN1531462 IHC ELISA WB Rabbit IgG AA 606-655, pThr638 Log in to see Polyclonal 1
12.306385 ABIN6271956 ELISA ICC IF WB Rabbit IgG pSer657 Log in to see Polyclonal 1
10.806385 ABIN6264241 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
10.806385 ABIN6254893 ELISA ICC IF IHC WB Rabbit IgG C-Term Log in to see Polyclonal 0
10.806385 ABIN6264243 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
10.806385 ABIN2428595 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
10.806385 ABIN2422039 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Protein Kinase C, alpha (PKCa) Antibodies
Epitope C-Term
(35), (20), (18), (17), (16), (16), (15), (14), (12), (10), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine), Rat (Rattus), Human
(309), (219), (176), (31), (26), (25), (21), (21), (20), (6), (3), (3), (2), (1), (1), (1), (1)
Host Rabbit
(277), (45), (2)
Clonality
Polyclonal
Conjugate This PKC alpha antibody is un-conjugated
(14), (13), (10), (7), (6), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(264), (154), (135), (71), (56), (50), (34), (32), (26), (24), (13), (5), (4), (4), (2), (1), (1), (1)
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Product Details anti-PKC alpha Antibody

Target Details PKC alpha Application Details Handling Images
Purification Affinity purification
Immunogen C term -peptide of human PRKCA
Isotype IgG
Plasmids, Primers & others

Target Details PKC alpha

Product Details anti-PKC alpha Antibody Application Details Handling Images back to top
Antigen
Alternative Name PRKCA (PKCa Antibody Abstract)
Background Activation of protein kinase C (PKC) is one of the earliest events in a cascade that controls a variety of cellular responses, including secretion, gene expression, proliferation, and muscle contraction. PKC isoforms belong to three groups based on calcium dependency and activators. Classical PKCs are calcium-dependent via their C2 domains and are activated by phosphatidylserine (PS), diacylglycerol (DAG), and phorbol esters (TPA, PMA) through their cysteine-rich C1 domains. Both novel and atypical PKCs are calcium-independent, but only novel PKCs are activated by PS, DAG, and phorbol esters. Members of these three PKC groups contain a pseudo-substrate or autoinhibitory domain that binds to substrate-binding sites in the catalytic domain to prevent activation in the absence of cofactors or activators. Control of PKC activity is regulated through three distinct phosphorylation events. Phosphorylation at Thr500 in the activation loop, the autophosphorylation site at Thr641, and at carboxy-terminal hydrophobic site Ser660 occurs in vivo. Atypical PKC isoforms lack hydrophobic region phosphorylation, which correlates with the presence of glutamic acid rather than the serine or threonine residues found in more typical PKC isoforms. Either the enzyme PDK1 or a close relative is responsible for PKC activation. A recent addition to the PKC superfamily is PKCmy (PKD), which is regulated by DAG and TPA through its C1 domain. PKD is distinguished by the presence of a PH domain and by its unique substrate recognition and Golgi localization. PKC-related kinases (PRK) lack the C1 domain and do not respond to DAG or phorbol esters. Phosphatidylinositol lipids activate PRKs and small Rho-family GTPases bind to the homology region 1 (HR1) to regulate PRK kinase activity.
Synonyms: PKCA, AAG6, PKCA, PRKACA, PKC-alpha, MGC129900, MGC129901, PRKCA
Gene ID 5578
UniProt P17252
Pathways WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-PKC alpha Antibody Target Details PKC alpha Handling Images back to top
Application Notes Recommended dilutions: WB: 1:500-1:2000, IHC: 1:50-1:200
Restrictions For Research Use only

Handling

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Images back to top
Format Liquid
Buffer PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze / thaw cycles
Storage -20 °C/-80 °C

Images

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Protein Kinase C, alpha (PKCa) (C-Term) antibody (ABIN3020835) Western blot analysis of extracts of Jurkat and K562 cells, using PRKCA antibody.
Western Blotting (WB) image for anti-Protein Kinase C, alpha (PKCa) (C-Term) antibody (ABIN3020835) Western blot analysis of extracts of various cell lines, using PRKCA antibody.
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Protein Kinase C, alpha (PKCa) (C-Term) antibody (ABIN3020835) Immunohistochemistry of paraffin-embedded human lung cancer using PRKCA antibody.
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Protein Kinase C, alpha (PKCa) (C-Term) antibody (ABIN3020835) Immunohistochemistry of paraffin-embedded human colon using PRKCA antibody.
Immunofluorescence (IF) image for anti-Protein Kinase C, alpha (PKCa) (C-Term) antibody (ABIN3020835) Immunofluorescence analysis of U2OS cells using PRKCA antibody.