anti-Rat (Rattus) PKC alpha antibody for Western Blotting

Recommended PKC alpha Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, alpha (PKCa) Antibodies
  • pkc-alpha
  • pkcalpha
  • im:7139045
  • si:dkey-8l19.1
  • LOC100219195
  • AAG6
  • PKC-alpha
  • PKCA
  • PRKACA
  • AI875142
  • Pkca
  • PKRCA
  • protein kinase C alpha
  • protein kinase C, alpha L homeolog
  • protein kinase C, alpha
  • PRKCA
  • prkca.L
  • prkca
  • Prkca
Epitope
AA 153-342
44
27
18
17
16
15
15
12
7
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
270
193
151
33
29
27
21
21
20
5
4
3
3
3
2
1
1
1
Host
Rabbit
232
46
2
Clonality
Polyclonal
Conjugate
This PKC alpha antibody is un-conjugated
11
11
9
7
6
5
5
5
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
226
124
98
69
51
44
26
26
24
23
5
4
4
3
2
1
1
1
1
1
Options
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Catalog No. ABIN3043549
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2908103 ABIN967751 IF IP IHC WB Mouse IgG2b, kappa AA 270-427 Log in to see 3-PKCalpha 5
3.2908103 ABIN1531462 IHC ELISA WB Rabbit IgG AA 606-655, pThr638 Log in to see Polyclonal 1
3.2908103 ABIN967750 IF IP IHC WB Mouse IgG2b, kappa AA 270-427 Log in to see 3-PKCalpha 6
3.2908103 ABIN1532477 IHC ELISA WB Rabbit IgG AA 606-655 Log in to see Polyclonal 0
3.2908103 ABIN1532520 ELISA WB Rabbit IgG AA 623-672 Log in to see Polyclonal 0
3.2908103 ABIN1531505 ELISA WB Rabbit IgG AA 623-672, pTyr657 Log in to see Polyclonal 0
3.2908103 ABIN1108731 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
3.2908103 ABIN1874290 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.2908103 ABIN256441 IP WB Mouse IgG2a kappa Log in to see PKC0103 0
3.2908103 ABIN498990 WB Rabbit Log in to see Polyclonal 0
3.2908103 ABIN3186466 ELISA IHC WB Rabbit IgG Thr287 Log in to see Polyclonal 0
3.2908103 ABIN3182587 ELISA IHC WB Rabbit IgG pThr638 Log in to see Polyclonal 0
3.2908103 ABIN3182236 ELISA WB Rabbit IgG pTyr657 Log in to see Polyclonal 0
3.2908103 ABIN4345808 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
3.2908103 ABIN3020835 IHC WB Rabbit IgG C-Term Log in to see Polyclonal 0
3.2908103 ABIN4345807 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
3.2908103 ABIN1844726 IHC WB Rabbit pThr638 Log in to see Polyclonal 0
3.2908103 ABIN4345801 IHC IHC (p) WB Rabbit Log in to see Polyclonal 0
3.2908103 ABIN4345805 WB Rabbit pThr638 Log in to see Polyclonal 0
3.2908103 ABIN3032372 WB Rabbit IgG AA 153-342 Log in to see Polyclonal 0

General

Antigen Protein Kinase C, alpha (PKCa) Antibodies
Epitope AA 153-342
(44), (27), (18), (17), (16), (15), (15), (12), (7), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(270), (193), (151), (33), (29), (27), (21), (21), (20), (5), (4), (3), (3), (3), (2), (1), (1), (1)
Host Rabbit
(232), (46), (2)
Clonality
Polyclonal
Conjugate This PKC alpha antibody is un-conjugated
(11), (11), (9), (7), (6), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(226), (124), (98), (69), (51), (44), (26), (26), (24), (23), (5), (4), (4), (3), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-PKC alpha Antibody

Target Details PKC alpha Application Details Handling References for anti-PKC alpha antibody (ABIN3043549) Images
Purpose Rabbit IgG polyclonal antibody for Protein kinase C alpha type(PRKCA) detection. Tested with WB in Human,Mouse,Rat.
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Protein kinase C alpha type(PRKCA) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: protein kinase C, alpha
Protein Name: Protein kinase C alpha type
Purification Immunogen affinity purified.
Immunogen E.coli-derived human PKC alpha recombinant protein (Position: M153-L342). Human PKC alpha shares 97% and 99% amino acid (aa) sequence identity with mouse and rat PKC alpha, respectively.
Isotype IgG

Target Details PKC alpha

Product Details anti-PKC alpha Antibody Application Details Handling References for anti-PKC alpha antibody (ABIN3043549) Images back to top
Antigen
Alternative Name PRKCA (PKCa Antibody Abstract)
Background Protein kinase C (PKC) is the major phorbol ester receptor. Activation of PKC by calcium ions and the second messenger diacylglycerol is thought to play a central role in the induction of cellular responses to a variety of ligand-receptor systems and in the regulation of cellular responsiveness to external stimuli. Three of these, termed alpha, beta and gamma, are highly homologous. PRKCA1 is mapped to 17q22-q23.2. PRKCA1 regulates cardiac contractility and propensity toward heart failure.

Synonyms: AAG6 antibody|Aging associated gene 6 antibody|aPKC antibody|KPCA_HUMAN antibody|MGC129900 antibody|MGC129901 antibody|PICK 1 antibody|PKC A antibody|PKC alpha antibody|PKC-A antibody|PKC-alpha antibody|PKCA antibody|PRKACA antibody|PRKC A antibody|PRKCA antibody|Protein Kinase C alpha antibody|Protein kinase C alpha type antibody
Gene ID 5578
UniProt P17252
Pathways WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-PKC alpha Antibody Target Details PKC alpha Handling References for anti-PKC alpha antibody (ABIN3043549) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for PKC alpha is approximately 0.25 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions For Research Use only

Handling

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details References for anti-PKC alpha antibody (ABIN3043549) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-PKC alpha antibody (ABIN3043549)

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling Images back to top
Product cited in:

Sun, Li, Ma, Liu, Xu, Ye, Hou, Wang, Li, Jiang: "The predictive value and potential mechanisms of miRNA-328 and miRNA-378 for brain metastases in operable and advanced non-small-cell lung cancer." in: Japanese journal of clinical oncology, Vol. 45, Issue 5, pp. 464-73, 2015

Cheng, Gu, Zhang, Yuan, Zhao, Jiang, Jia: "Astragaloside IV inhibits migration and invasion in human lung cancer A549 cells via regulating PKC-?-ERK1/2-NF-?B pathway." in: International immunopharmacology, Vol. 23, Issue 1, pp. 304-13, 2015

Images

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling References for anti-PKC alpha antibody (ABIN3043549) back to top
Supplier Images
Western Blotting (WB) image for anti-Protein Kinase C, alpha (PKCa) (AA 153-342) antibody (ABIN3043549) anti-Protein Kinase C, alpha (PKCa) (AA 153-342) antibody