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|Antigen||Protein Kinase C, beta (PRKCB) Antibodies|
|Epitope||AA 126-324 Alternatives|
|Reactivity||Chicken, Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This PKC beta antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
|5 references available|
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Product Details anti-PKC beta AntibodyTarget Details PKC beta Application Details Handling References for anti-PKC beta antibody (ABIN967768) Images
|Cross-Reactivity||Rat (Rattus), Mouse (Murine), Chicken|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Human PKCbeta aa. 126-324|
Target Details PKC betaProduct Details anti-PKC beta Antibody Application Details Handling References for anti-PKC beta antibody (ABIN967768) Images back to top
|Alternative Name||PKC beta (PRKCB Antibody Abstract)|
|Background||The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. These proteins are products of multiple genes and alternative splicing. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with the PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester. However, the novel PKC (nPKC) subfamily members ( delta, epsilon, eta, and theta isoforms) and the atypical PKC (aPKC) subfamily members (zeta, iota, and lambda isoforms) are Ca2+ independent and lack the C2 domain. The aPKC members are unique in that their activity is independent of diacylglycerols and phorbol esters. They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC. The C-terminal region of PKC contains the catalytic domain. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. PKCbeta is highly expressed in brain and hematopoietic cells. Autophosphorylation of PKCbeta occurs at the N- and C-terminal regions, as well as within the hinge region. However, only the COOH- terminal autophosphorylation sites are essential for PKCbeta's function and subcellular localization. PKCbeta is critical for the proliferation of K562 cells, as well as being an important regulator of human melanogenesis.|
|Pathways||WNT Signaling, TCR Signaling, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded protein response|
Application DetailsProduct Details anti-PKC beta Antibody Target Details PKC beta Handling References for anti-PKC beta antibody (ABIN967768) Images back to top
Related Products: ABIN968545, ABIN967389
|Restrictions||For Research Use only|
HandlingProduct Details anti-PKC beta Antibody Target Details PKC beta Application Details References for anti-PKC beta antibody (ABIN967768) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20° C.|
References for anti-PKC beta antibody (ABIN967768)Product Details anti-PKC beta Antibody Target Details PKC beta Application Details Handling Images back to top
|Product cited in:||
Masur, Lang, Niggemann, Zanker, Entschladen: "High PKC alpha and low E-cadherin expression contribute to high migratory activity of colon carcinoma cells." in: Molecular biology of the cell, Vol. 12, Issue 7, pp. 1973-82, 2001
Stebbins, Mochly-Rosen: "Binding specificity for RACK1 resides in the V5 region of beta II protein kinase C." in: The Journal of biological chemistry, Vol. 276, Issue 32, pp. 29644-50, 2001
Bell, Burns: "Lipid activation of protein kinase C." in: The Journal of biological chemistry, Vol. 266, Issue 8, pp. 4661-4, 1991
Soderling: "Protein kinases. Regulation by autoinhibitory domains." in: The Journal of biological chemistry, Vol. 265, Issue 4, pp. 1823-6, 1990
Nishizuka: "The molecular heterogeneity of protein kinase C and its implications for cellular regulation." in: Nature, Vol. 334, Issue 6184, pp. 661-5, 1988
ImagesProduct Details anti-PKC beta Antibody Target Details PKC beta Application Details Handling References for anti-PKC beta antibody (ABIN967768) back to top