anti-Mouse (Murine) PKC beta antibody for Immunocytochemistry

Recommended PKC beta Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, beta (PRKCB) Antibodies
  • PKC-beta
  • PKCB
  • PRKCB1
  • PRKCB2
  • A130082F03Rik
  • PKC-Beta
  • Pkcb
  • Prkcb1
  • Prkcb2
  • PKC-B
  • Prkcb
  • prkcb1
  • zgc:63591
  • PKC
  • PRKCB_tv2
  • Pkc53E
  • prkcb1l
  • zgc:64063
  • protein kinase C beta
  • protein kinase C, beta
  • protein kinase C, beta b
  • protein kinase C, beta a
  • PRKCB
  • Prkcb
  • prkcbb
  • prkcba
Epitope
pSer661
38
16
16
15
15
14
11
9
9
9
7
6
5
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
196
137
136
23
20
14
10
5
4
3
2
2
2
1
1
1
Host
Rabbit
212
18
8
5
1
Clonality
Polyclonal
Conjugate
This PKC beta antibody is un-conjugated
18
16
8
6
6
6
2
2
2
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
215
115
105
27
24
17
13
13
11
8
4
3
3
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN6255455
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7 ABIN6271958 ELISA ICC IF WB Rabbit IgG pThr500 Log in to see Polyclonal 0
7 ABIN6264249 ELISA ICC IF WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN220839 ELISA ICC IHC IHC (fro) IHC (p) WB Rabbit IgG AA 660-673 Log in to see Polyclonal 0

General

Antigen Protein Kinase C, beta (PRKCB) Antibodies
Epitope pSer661
(38), (16), (16), (15), (15), (14), (11), (9), (9), (9), (7), (6), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(196), (137), (136), (23), (20), (14), (10), (5), (4), (3), (2), (2), (2), (1), (1), (1)
Host Rabbit
(212), (18), (8), (5), (1)
Clonality
Polyclonal
Conjugate This PKC beta antibody is un-conjugated
(18), (16), (8), (6), (6), (6), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(215), (115), (105), (27), (24), (17), (13), (13), (11), (8), (4), (3), (3), (1), (1)
Supplier Log in to see

Product Details anti-PKC beta Antibody

Target Details PKC beta Application Details Handling Images
Specificity Phospho-PKCB (Ser661) Antibody detects endogenous levels of PKCB only when phosphorylated at Serine 661
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen A synthesized peptide derived from human PKCB around the phosphorylation site of Serine 661
Isotype IgG
Plasmids, Primers & others

Target Details PKC beta

Product Details anti-PKC beta Antibody Application Details Handling Images back to top
Antigen
Alternative Name PKCB (PRKCB Antibody Abstract)
Background

Description: Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. May participate in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (By similarity). Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription.

Gene: PRKCB

Molecular Weight 77kDa
Gene ID 5579
UniProt P05771
Pathways WNT Signaling, TCR Signaling, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded protein response

Application Details

Product Details anti-PKC beta Antibody Target Details PKC beta Handling Images back to top
Application Notes WB 1:500-1:2000, IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-PKC beta Antibody Target Details PKC beta Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

Images

Product Details anti-PKC beta Antibody Target Details PKC beta Application Details Handling back to top
Supplier Images
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Protein Kinase C, beta (PRKCB) (pSer661) antibody (ABIN6255455) ABIN6267310 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized...
Western Blotting (WB) image for anti-Protein Kinase C, beta (PRKCB) (pSer661) antibody (ABIN6255455) Western blot analysis of PKCB phosphorylation expression in heatshock treated HeLa wh...