anti-Monkey Rhodopsin antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Rhodopsin Antibody (supplied by: Log in to see )

Antigen
Rhodopsin (RHO) Antibodies
  • CSNBAD1
  • OPN2
  • RP4
  • RH1
  • Rh
  • fi06d11
  • wu:fi06d11
  • zfo2
  • zfrho
  • Noerg1
  • Opn2
  • Ops
  • opn2
  • rh1
  • rp4
  • xrho
  • PIGRHO1
  • ops
  • RDP1
  • RHO
  • rhodopsin
  • rhodopsin L homeolog
  • RHO
  • Rho
  • rho
  • rho.L
Epitope
Extracellular Domain
30
19
11
7
7
5
3
3
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
157
59
52
52
39
36
36
35
22
22
21
18
18
16
4
2
2
1
1
1
1
Host
Rabbit
239
73
5
1
Clonality
Polyclonal
Conjugate
This Rhodopsin antibody is un-conjugated
15
14
13
13
12
10
9
6
6
6
6
6
6
6
6
6
5
5
5
5
5
5
5
4
4
4
4
4
2
2
2
2
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
295
218
179
164
153
134
58
40
3
2
1
1
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Catalog No. ABIN1049292
$ 484.00
Plus shipping costs $45.00

General

Antigen Rhodopsin (RHO) Antibodies
Epitope Extracellular Domain
(30), (19), (11), (7), (7), (5), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(157), (59), (52), (52), (39), (36), (36), (35), (22), (22), (21), (18), (18), (16), (4), (2), (2), (1), (1), (1), (1)
Host Rabbit
(239), (73), (5), (1)
Clonality
Polyclonal
Conjugate This Rhodopsin antibody is un-conjugated
(15), (14), (13), (13), (12), (10), (9), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (2), (2), (2), (2)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(295), (218), (179), (164), (153), (134), (58), (40), (3), (2), (1), (1)
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Product Details anti-Rhodopsin Antibody

Target Details Rhodopsin Application Details Handling Images
Brand IHC-plus™
Specificity Human RHO / Rhodopsin. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Monkey, Mouse, Rat, Rabbit, Pig, Cat, Elephant, Zebra finch (100%).
Purification Immunoaffinity purified
Immunogen Synthetic 19 amino acid peptide from 2nd extracellular domain of human RHO / Rhodopsin. Percent identity with other species by BLAST analysis: Human, Monkey, Mouse, Rat, Rabbit, Pig, Cat, Elephant, Zebra finch (100%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details Rhodopsin

Product Details anti-Rhodopsin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Rhodopsin / rho (RHO Antibody Abstract)
Target Type Chemical
Background Name/Gene ID: RHO
Subfamily: Opsin
Family: GPCR

Synonyms: RHO, CSNBAD1, OPN2, Rhodopsin, RP4, Opsin 2, rod pigment, Opsin-2
Gene ID 6010
Pathways WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction

Application Details

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Handling Images back to top
Application Notes Approved: IHC, IHC-P (1 - 3 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Rhodopsin (RHO) (Extracellular Domain) antibody (ABIN1049292) Anti-RHO / Rhodopsin antibody ABIN1049292 IHC staining of human eye, retina. Immunoh...
Immunohistochemistry (IHC) image for anti-Rhodopsin (RHO) (Extracellular Domain) antibody (ABIN1049292) Anti-RHO / Rhodopsin antibody IHC of human eye, retina. Immunohistochemistry of forma...