anti-Shark Rhodopsin antibody for Western Blotting

Recommended Rhodopsin Antibody (supplied by: Log in to see )

Antigen
Rhodopsin (RHO) Antibodies
  • CSNBAD1
  • OPN2
  • RP4
  • RH1
  • Rh
  • fi06d11
  • wu:fi06d11
  • zfo2
  • zfrho
  • Noerg1
  • Opn2
  • Ops
  • opn2
  • rh1
  • rp4
  • xrho
  • PIGRHO1
  • ops
  • RDP1
  • RHO
  • rhodopsin
  • rhodopsin L homeolog
  • RHO
  • Rho
  • rho
  • rho.L
Reactivity
Amphibian, Bird (Avian), Fish, Mammalian, Shark
96
59
54
53
53
51
51
41
39
38
35
23
18
16
4
2
2
2
2
1
1
Host
Mouse
217
74
5
1
Clonality
Monoclonal
Conjugate
This Rhodopsin antibody is un-conjugated
15
14
12
9
9
9
6
6
6
6
6
6
6
6
6
6
4
4
4
4
4
4
4
4
4
4
3
3
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
274
205
163
139
128
124
42
40
6
2
1
1
1
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Pubmed
4 references available
Catalog No. ABIN863083
$ 453.70
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
15.38187 ABIN2482261 ICC IF IP IHC ELISA WB APC Mouse IgG1 Log in to see 4D2 0
15.38187 ABIN2482263 ICC IF IP IHC ELISA WB FITC Mouse IgG1 Log in to see 4D2 0
15.38187 ABIN2482264 ICC IF IP IHC ELISA WB HRP Mouse IgG1 Log in to see 4D2 0
15.38187 ABIN2482265 ICC IF IP IHC ELISA WB PE-Atto 594 Mouse IgG1 Log in to see 4D2 0
15.38187 ABIN2482252 ICC IF IP IHC ELISA WB Atto 390 Mouse IgG1 Log in to see 4D2 0
15.38187 ABIN2482254 ICC IF IP IHC ELISA WB Atto 565 Mouse IgG1 Log in to see 4D2 0
15.38187 ABIN2482255 ICC IF IP IHC ELISA WB Atto 594 Mouse IgG1 Log in to see 4D2 0
15.38187 ABIN2482256 ICC IF IP IHC ELISA WB Atto 633 Mouse IgG1 Log in to see 4D2 0
15.38187 ABIN2482257 ICC IF IP IHC ELISA WB Atto 655 Mouse IgG1 Log in to see 4D2 0
15.38187 ABIN2482259 ICC IF IP IHC ELISA WB Atto 700 Mouse IgG1 Log in to see 4D2 0
15.38187 ABIN2482262 ICC IF IP IHC ELISA WB Biotin Mouse IgG1 Log in to see 4D2 0
15.38187 ABIN2482266 ICC IF IP IHC ELISA WB PerCP Mouse IgG1 Log in to see 4D2 0
15.38187 ABIN2482268 ICC IF IP IHC ELISA WB Streptavidin Mouse IgG1 Log in to see 4D2 0
15.38187 ABIN2482267 ICC IF IP IHC ELISA WB PE Mouse IgG1 Log in to see 4D2 0
15.38187 ABIN2482253 ICC IF IP IHC ELISA WB Atto 488 Mouse IgG1 Log in to see 4D2 0
15.38187 ABIN2482258 ICC IF IP IHC ELISA WB Atto 680 Mouse IgG1 Log in to see 4D2 0
15.38187 ABIN2482260 ICC IF IP IHC ELISA WB Alkaline Phosphatase (AP) Mouse IgG1 Log in to see 4D2 0
1 ABIN5873302 ELISA IF/ICC IHC IP WB Atto 488 Mouse IgG1 Log in to see 0
1 ABIN5873304 ELISA IF/ICC IHC IP WB Atto 565 Mouse IgG1 Log in to see 0
1 ABIN5873306 ELISA IF/ICC IHC IP WB Atto 594 Mouse IgG1 Log in to see 0

General

Antigen Rhodopsin (RHO) Antibodies
Reactivity Amphibian, Bird (Avian), Fish, Mammalian, Shark
(96), (59), (54), (53), (53), (51), (51), (41), (39), (38), (35), (23), (18), (16), (4), (2), (2), (2), (2), (1), (1)
Host Mouse
(217), (74), (5), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Rhodopsin antibody is un-conjugated
(15), (14), (12), (9), (9), (9), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(274), (205), (163), (139), (128), (124), (42), (40), (6), (2), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-Rhodopsin Antibody

Target Details Rhodopsin Application Details Handling References for anti-Rhodopsin antibody (ABIN863083) Images
Specificity Detects ~40 kDa. Binds specifically to the N-terminus of Rhodopsin. Does not detect Rhodopsin in invertebrates.
Purification Protein G Purified
Immunogen Bovine Rhodopsin
Clone 4D2
Isotype IgG1
Plasmids, Primers & others

Target Details Rhodopsin

Product Details anti-Rhodopsin Antibody Application Details Handling References for anti-Rhodopsin antibody (ABIN863083) Images back to top
Antigen
Alternative Name Rhodopsin (RHO Antibody Abstract)
Target Type Chemical
Background Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).
Gene ID 509933
NCBI Accession NP_001014890
UniProt P02699
Pathways WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction

Application Details

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Handling References for anti-Rhodopsin antibody (ABIN863083) Images back to top
Application Notes
  • WB (1:1000)
  • IHC (1000)
  • optimal dilutions for assays should be determined by the user.
Comment

1 μg/ml of SMC-176 was sufficient for detection of rhodopsin in 10 μg of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Restrictions For Research Use only

Handling

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details References for anti-Rhodopsin antibody (ABIN863083) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

References for anti-Rhodopsin antibody (ABIN863083)

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details Handling Images back to top
Product cited in:

Tanaka, Parker, Hasegawa, Siveter, Yamamoto, Miyashita, Takahashi, Ito, Wakamatsu, Mukuda, Matsuura, Tomikawa, Furutani, Suzuki, Maeda: "Mineralized rods and cones suggest colour vision in a 300 Myr-old fossil fish." in: Nature communications, Vol. 5, pp. 5920, 2014

Background publications

Iannaccone, Man, Waseem, Jennings, Ganapathiraju, Gallaher, Reese, Bhattacharya, Klein-Seetharaman: "Retinitis pigmentosa associated with rhodopsin mutations: Correlation between phenotypic variability and molecular effects." in: Vision research, Vol. 46, Issue 27, pp. 4556-67, 2006

Ridge, Lee, Abdulaev: "Examining rhodopsin folding and assembly through expression of polypeptide fragments." in: The Journal of biological chemistry, Vol. 271, Issue 13, pp. 7860-7, 1996

Molday, Hicks, Molday: "Peripherin. A rim-specific membrane protein of rod outer segment discs." in: Investigative ophthalmology & visual science, Vol. 28, Issue 1, pp. 50-61, 1987