anti-Vertebrate Rhodopsin antibody for Immunohistochemistry

Recommended Rhodopsin Antibody (supplied by: Log in to see )

Antigen
Rhodopsin (RHO) Antibodies
  • CSNBAD1
  • OPN2
  • RP4
  • RH1
  • Rh
  • fi06d11
  • wu:fi06d11
  • zfo2
  • zfrho
  • Noerg1
  • Opn2
  • Ops
  • opn2
  • rh1
  • rp4
  • xrho
  • PIGRHO1
  • ops
  • RDP1
  • RHO
  • rhodopsin
  • rhodopsin L homeolog
  • RHO
  • Rho
  • rho
  • rho.L
Reactivity
Vertebrate
96
59
55
54
52
51
51
41
40
39
36
23
18
16
4
2
2
2
2
1
1
Host
Mouse
217
74
5
1
Clonality
Monoclonal
Conjugate
This Rhodopsin antibody is conjugated to Atto 488
15
14
12
9
9
9
6
6
6
6
6
6
6
6
6
5
4
4
4
4
4
4
4
4
4
4
3
3
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
274
205
163
139
128
124
42
40
6
2
1
1
1
Options
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Available images

Catalog No. ABIN2482270
$ 514.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
21.045391 ABIN2482269 ICC IF IP IHC ELISA WB Atto 390 Mouse IgG1 Log in to see 1D4 0
21.045391 ABIN2482272 ICC IF IP IHC ELISA WB Atto 594 Mouse IgG1 Log in to see 1D4 0
21.045391 ABIN2482271 ICC IF IP IHC ELISA WB Atto 565 Mouse IgG1 Log in to see 1D4 0
21.045391 ABIN2482275 ICC IF IP IHC ELISA WB Atto 680 Mouse IgG1 Log in to see 1D4 0
21.045391 ABIN2482277 ICC IF IP IHC ELISA WB Alkaline Phosphatase (AP) Mouse IgG1 Log in to see 1D4 0
21.045391 ABIN2482278 ICC IF IP IHC ELISA WB APC Mouse IgG1 Log in to see 1D4 0
21.045391 ABIN2482281 ICC IF IP IHC ELISA WB HRP Mouse IgG1 Log in to see 1D4 0
21.045391 ABIN2482282 ICC IF IP IHC ELISA WB PE-Atto 594 Mouse IgG1 Log in to see 1D4 0
21.045391 ABIN2482284 ICC IF IP IHC ELISA WB PE Mouse IgG1 Log in to see 1D4 0
21.045391 ABIN2482279 ICC IF IP IHC ELISA WB Biotin Mouse IgG1 Log in to see 1D4 0
21.045391 ABIN2482276 ICC IF IP IHC ELISA WB Atto 700 Mouse IgG1 Log in to see 1D4 0
21.045391 ABIN2482273 ICC IF IP IHC ELISA WB Atto 633 Mouse IgG1 Log in to see 1D4 0
21.045391 ABIN2482274 ICC IF IP IHC ELISA WB Atto 655 Mouse IgG1 Log in to see 1D4 0
21.045391 ABIN2482280 ICC IF IP IHC ELISA WB FITC Mouse IgG1 Log in to see 1D4 0
21.045391 ABIN2482283 ICC IF IP IHC ELISA WB PerCP Mouse IgG1 Log in to see 1D4 0
21.045391 ABIN2482285 ICC IF IP IHC ELISA WB Streptavidin Mouse IgG1 Log in to see 1D4 0
19.573498 ABIN863085 ICC IF IP IHC ELISA WB Mouse IgG1 Log in to see 1D4 4
1 ABIN1103990 ICC IP IHC ELISA WB Mouse IgG1 Log in to see Rho 1D4 0
1 ABIN1952429 ICC IF IP IHC ELISA WB Atto 488 Mouse IgG1 Log in to see 1D4 0
1 ABIN1952518 ICC IF IP IHC ELISA WB PerCP Mouse IgG1 Log in to see 1D4 0

General

Antigen Rhodopsin (RHO) Antibodies
Reactivity Vertebrate
(96), (59), (55), (54), (52), (51), (51), (41), (40), (39), (36), (23), (18), (16), (4), (2), (2), (2), (2), (1), (1)
Host Mouse
(217), (74), (5), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Rhodopsin antibody is conjugated to Atto 488
(15), (14), (12), (9), (9), (9), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(274), (205), (163), (139), (128), (124), (42), (40), (6), (2), (1), (1), (1)
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Product Details anti-Rhodopsin Antibody

Target Details Rhodopsin Application Details Handling Images
Specificity Detects ~40 kDa. Binds specifically to the N-terminus of Rhodopsin. Does not detect Rhodopsin in invertebrates.
Purification Protein G Purified
Immunogen Bovine Rhodopsin
Clone 1D4
Isotype IgG1
Plasmids, Primers & others

Target Details Rhodopsin

Product Details anti-Rhodopsin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Rhodopsin (RHO Antibody Abstract)
Target Type Chemical
Background Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).
Gene ID 509933
NCBI Accession NP_001014890
UniProt P02699
Pathways WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction

Application Details

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Handling Images back to top
Application Notes
  • WB (1:1000)
  • IHC (1:100)
  • optimal dilutions for assays should be determined by the user.
Comment

1 μg/ml of SMC-177 was sufficient for detection of rhodopsin in 10 μg of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions For Research Use only

Handling

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

Images

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Rhodopsin (RHO) antibody (Atto 488) (ABIN2482270) Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1...
Western Blotting (WB) image for anti-Rhodopsin (RHO) antibody (Atto 488) (ABIN2482270) Western Blot analysis of Human Cell lysates showing detection of Rhodopsin protein us...