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|Antigen||Transcription Factor 4 (TCF4) Antibodies|
|Epitope||AA 31-331 Alternatives|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This TCF4 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
|5 references available|
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Product Details anti-TCF4 AntibodyTarget Details TCF4 Application Details Handling References for anti-TCF4 antibody (ABIN264393) Images
|Characteristics||Synonyms: Transcription factor 4, TCF-4, ITF2, ITF-2, SEF2, SEF-2, Immunoglobulin transcription factor2, SL3-3 enhancer factor 2|
|Immunogen||Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized withrecombinant protein corresponding to amino acids 31-331 of the human TCF-4 protein. Genename: TCF7L2|
Target Details TCF4Product Details anti-TCF4 Antibody Application Details Handling References for anti-TCF4 antibody (ABIN264393) Images back to top
|Alternative Name||TCF4 (TCF4 Antibody Abstract)|
|Background||TCF-4 (T Cell Factor 4) is a member of the TCF/LEF (Lymphoid Enhancer Factor) family of transcription factors. Members of this family along with catenin, are involved in mediating Wnt signaling by transactivating downstream genes. TCF-4 generates transcriptionally active complexes with beta catenin in colon carcinoma cells. Expression of TCF-4 is highest in the CNS and intestinal epithelium where Wnt signaling also plays an essential role in development. Aberrant TCF-4 transcription activity has been implicated in carcinogenesis of intestinal and mammary epithlia.Synonyms: ITF-2, ITF2, Immunoglobulin transcription factor 2, SEF-2, SEF2, SL3-3 enhancer factor 2, TCF-4, Transcription factor 4|
|Pathways||WNT Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Regulation of Hormone Metabolic Process, Carbohydrate Homeostasis, Stem Cell Maintenance, Protein targeting to Nucleus|
Application DetailsProduct Details anti-TCF4 Antibody Target Details TCF4 Handling References for anti-TCF4 antibody (ABIN264393) Images back to top
Western Blotting: Detects a 66 kDa band in Cos-1 cells transfected with human TCF-4 (1-4g/mL). Immunoprecipitation. Immunohistochemistry of 4 % paraformaldehyde fixed tissues. Gel Shift.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-TCF4 Antibody Target Details TCF4 Application Details References for anti-TCF4 antibody (ABIN264393) Images back to top
|Buffer||0.7 M Tris-Glycine, pH 7.4, 0.107 M Sodium Chloride, 30 % Glycerol, 0.035 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
|Expiry Date||12 months|
References for anti-TCF4 antibody (ABIN264393)Product Details anti-TCF4 Antibody Target Details TCF4 Application Details Handling Images back to top
|Product cited in:||
Shimura, Takenaka, Tsutsumi, Hogan, Kikuchi, Raz: "Galectin-3, a novel binding partner of beta-catenin." in: Cancer research, Vol. 64, Issue 18, pp. 6363-7, 2004
Uematsu, Kanazawa, You, He, Xu, Li, Peterlin, McCormick, Jablons: "Wnt pathway activation in mesothelioma: evidence of Dishevelled overexpression and transcriptional activity of beta-catenin." in: Cancer research, Vol. 63, Issue 15, pp. 4547-51, 2003
Barker, Huls, Korinek, Clevers: "Restricted high level expression of Tcf-4 protein in intestinal and mammary gland epithelium." in: The American journal of pathology, Vol. 154, Issue 1, pp. 29-35, 1999
Ishitani, Ninomiya-Tsuji, Nagai, Nishita, Meneghini, Barker, Waterman, Bowerman, Clevers, Shibuya, Matsumoto: "The TAK1-NLK-MAPK-related pathway antagonizes signalling between beta-catenin and transcription factor TCF." in: Nature, Vol. 399, Issue 6738, pp. 798-802, 1999
Korinek, Barker, Moerer, van Donselaar, Huls, Peters, Clevers: "Depletion of epithelial stem-cell compartments in the small intestine of mice lacking Tcf-4." in: Nature genetics, Vol. 19, Issue 4, pp. 379-83, 1998
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