anti-Human WNT1 antibody for Western Blotting

Recommended WNT1 Antibody (supplied by: Log in to see )

Antigen
Wingless-Type MMTV Integration Site Family, Member 1 (WNT1) Antibodies
  • Xint-1
  • Xwnt1
  • int-1
  • int1
  • wnt-1
  • wnt1-a
  • Int-1
  • Wnt-1
  • sw
  • swaying
  • BMND16
  • INT1
  • OI15
  • Int1
  • WNT-1
  • sb:eu647
  • zgc:194464
  • zgc:194478
  • WNT1
  • Wnt family member 1
  • Wnt family member 1 L homeolog
  • wingless-type MMTV integration site family, member 1
  • WNT1
  • wnt1.L
  • Wnt1
  • wnt1
Epitope
AA 45-74, N-Term
32
18
15
4
4
2
2
2
1
1
1
1
1
Reactivity
Human
135
51
20
12
8
7
7
6
6
4
3
3
2
2
1
1
Host
Rabbit
115
20
Clonality
Polyclonal
Conjugate
This WNT1 antibody is un-conjugated
8
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
113
58
46
28
20
18
7
6
6
4
1
1
1
Options
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Catalog No. ABIN3042350
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1493068 ABIN2779465 WB Rabbit Middle Region Log in to see Polyclonal 1
3.1493068 ABIN2779466 WB Rabbit C-Term Log in to see Polyclonal 0
3.1493068 ABIN733748 IF (p) IHC (p) WB Rabbit IgG AA 255-300 Log in to see Polyclonal 4
3.1493068 ABIN223266 WB Rabbit IgG Log in to see Polyclonal 0
3.1493068 ABIN2705330 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal 0
3.1493068 ABIN964685 WB Mouse IgG1 kappa Internal Region Log in to see 13F9 0
3.1493068 ABIN2856226 WB Rabbit IgG Center Log in to see Polyclonal 0
3.1493068 ABIN408121 ICC IF IHC IHC (fro) IHC (p) WB Rabbit Log in to see Polyclonal 0
3.1493068 ABIN390755 FACS IHC (p) WB Rabbit Ig Fraction AA 260-288, C-Term Log in to see Polyclonal 1
3.1493068 ABIN969458 ICC FACS IHC ELISA WB Mouse IgG1 Log in to see 10C8 1
3.1493068 ABIN1846734 ELISA FACS IF/ICC IHC WB Mouse IgG1 Log in to see 0
3.1493068 ABIN453464 EIA WB Rabbit C-Term Log in to see Polyclonal 1
3.1493068 ABIN233837 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 0
3.1493068 ABIN3187496 ELISA IF IHC WB Rabbit IgG C-Term Log in to see Polyclonal 0
3.1493068 ABIN635697 WB Rabbit Middle Region Log in to see Polyclonal 0
3.1493068 ABIN1109511 EIA IF IHC (p) WB Mouse IgG1 Log in to see 10C8 0
3.1493068 ABIN4905627 WB Rabbit IgG Log in to see Polyclonal 0
3.1493068 ABIN3029624 FACS IHC ELISA WB Rabbit Ig Fraction AA 260-288 Log in to see Polyclonal 0
3.1493068 ABIN2432505 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
3.1493068 ABIN3016106 FACS IHC WB Rabbit Log in to see Polyclonal 0

General

Antigen Wingless-Type MMTV Integration Site Family, Member 1 (WNT1) Antibodies
Epitope AA 45-74, N-Term
(32), (18), (15), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(135), (51), (20), (12), (8), (7), (7), (6), (6), (4), (3), (3), (2), (2), (1), (1)
Host Rabbit
(115), (20)
Clonality
Polyclonal
Conjugate This WNT1 antibody is un-conjugated
(8), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(113), (58), (46), (28), (20), (18), (7), (6), (6), (4), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-WNT1 Antibody

Target Details WNT1 Application Details Handling References for anti-WNT1 antibody (ABIN3042350) Images
Purpose Rabbit IgG polyclonal antibody for Proto-oncogene Wnt-1(WNT1) detection. Tested with WB in Human.
Sequence NLLTDSKSLQ LVLEPSLQLL SRKQRRLIRQ
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Proto-oncogene Wnt-1(WNT1) detection. Tested with WB in Human.
Gene Name: wingless-type MMTV integration site family, member 1
Protein Name: Proto-oncogene Wnt-1
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human Wnt1 (45-74aa NLLTDSKSLQLVLEPSLQLLSRKQRRLIRQ), identical to the related mouse and rat sequences.
Isotype IgG
Plasmids, Primers & others

Target Details WNT1

Product Details anti-WNT1 Antibody Application Details Handling References for anti-WNT1 antibody (ABIN3042350) Images back to top
Antigen
Alternative Name WNT1 (WNT1 Antibody Abstract)
Background Wingless-type MMIV integration site family, member 1 is a protein that in humans is encoded by the WNT1 gene. This gene is a member of the WNT gene family. The gene was assigned to human chromosome 12q13.12. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. The Wnt1 protein functions in the induction of the mesencephalon and cerebellum. However, the gene mutations might not have a significant role in Joubert syndrome.

Synonyms: BMND16 antibody|INT1 antibody|OI15 antibody|oncogene Int1 antibody|Proto oncogene protein Wnt 1 antibody|Proto-oncogene Int-1 homolog antibody|Proto-oncogene Wnt-1 antibody|Wingless type MMTV integration site family member 1 antibody|wingless-type MMTV integration site family, member 1 (oncogene INT1) antibody|Wnt 1 antibody|wnt1 antibody|WNT1_HUMAN antibody
Gene ID 7471
UniProt P04628
Pathways WNT Signaling, Dopaminergic Neurogenesis

Application Details

Product Details anti-WNT1 Antibody Target Details WNT1 Handling References for anti-WNT1 antibody (ABIN3042350) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Wnt1 is approximately 0.1 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions For Research Use only

Handling

Product Details anti-WNT1 Antibody Target Details WNT1 Application Details References for anti-WNT1 antibody (ABIN3042350) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-WNT1 antibody (ABIN3042350)

Product Details anti-WNT1 Antibody Target Details WNT1 Application Details Handling Images back to top
Product cited in:

Mi, Li, Zhang, Zhao, Peng, Yang, Zheng: "MicroRNA-139-5p regulates C2C12 cell myogenesis through blocking Wnt/β-catenin signaling pathway." in: Biochemistry and cell biology = Biochimie et biologie cellulaire, Vol. 93, Issue 1, pp. 8-15, 2015

Dong, Duan, Han, Zhang, Wu: "Suppression of wingless-type MMTV integration site family, member 1 expression by small interfering RNA inhibits U251 glioma cell growth in vitro." in: Oncology letters, Vol. 9, Issue 1, pp. 81-85, 2014

Images

Product Details anti-WNT1 Antibody Target Details WNT1 Application Details Handling References for anti-WNT1 antibody (ABIN3042350) back to top
Supplier Images
Western Blotting (WB) image for anti-Wingless-Type MMTV Integration Site Family, Member 1 (WNT1) (AA 45-74), (N-Term) antibody (ABIN3042350) anti-Wingless-Type MMTV Integration Site Family, Member 1 (WNT1) (AA 45-74), (N-Term) antibody