anti-Monkey WNT10A antibody for Immunohistochemistry

Recommended WNT10A Antibody (supplied by: Log in to see )

Antigen
Wingless-Type MMTV Integration Site Family, Member 10A (WNT10A) Antibodies
  • WNT10A
  • wnt10a/12
  • wnt10a
  • OODD
  • SSPS
  • STHAG4
  • WNT-10A
  • Wnt family member 10A
  • protein Wnt-10a
  • Wnt10a protein
  • hypothetical protein
  • WNT10A protein
  • Wnt family member 10A L homeolog
  • wingless-type MMTV integration site family, member 10A
  • wingless-type MMTV integration site family, member 10a
  • WNT10A
  • LOC778792
  • CpipJ_CPIJ005839
  • Wnt10a
  • wnt10a
  • wnt10a.L
Epitope
Internal Region
11
6
6
4
2
2
2
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Monkey
51
24
11
6
6
6
4
4
2
2
1
1
1
1
Host
Rabbit
57
1
Clonality
Polyclonal
Conjugate
This WNT10A antibody is un-conjugated
5
3
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
36
29
20
15
6
6
5
3
1
1
1
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Catalog No. ABIN1049468
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.672434 ABIN1049467 IHC IHC (p) Rabbit Internal Region Log in to see Polyclonal 0
8.672434 ABIN2469512 IHC Rabbit Internal Region Log in to see Polyclonal 0
1 ABIN213598 IHC IHC (p) Rabbit Internal Region Log in to see Polyclonal 0

General

Antigen Wingless-Type MMTV Integration Site Family, Member 10A (WNT10A) Antibodies
Epitope Internal Region
(11), (6), (6), (4), (2), (2), (2), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Monkey
(51), (24), (11), (6), (6), (6), (4), (4), (2), (2), (1), (1), (1), (1)
Host Rabbit
(57), (1)
Clonality
Polyclonal
Conjugate This WNT10A antibody is un-conjugated
(5), (3), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(36), (29), (20), (15), (6), (6), (5), (3), (1), (1), (1)
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Product Details anti-WNT10A Antibody

Target Details WNT10A Application Details Handling Images
Brand IHC-plus™
Specificity Human WNT10A. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Dog, Bovine (100%) Mouse, Rat, Hamster, Bat, Rabbit (94%) Opossum (81%).
Purification Immunoaffinity purified
Immunogen Synthetic 16 amino acid peptide from internal region of human WNT10A. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Dog, Bovine (100%), Mouse, Rat, Hamster, Bat, Rabbit (94%), Opossum (81%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details WNT10A

Product Details anti-WNT10A Antibody Application Details Handling Images back to top
Antigen
Alternative Name WNT10A (WNT10A Antibody Abstract)
Background Name/Gene ID: WNT10A
Family: WNT

Synonyms: WNT10A, SSPS, STHAG4, OODD, Protein Wnt-10a
Gene ID 80326
Pathways WNT Signaling

Application Details

Product Details anti-WNT10A Antibody Target Details WNT10A Handling Images back to top
Application Notes Approved: IHC, IHC-P (5 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-WNT10A Antibody Target Details WNT10A Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-WNT10A Antibody Target Details WNT10A Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family, Member 10A (WNT10A) (Internal Region) antibody (ABIN1049468) Anti-WNT10A antibody ABIN1049468 IHC staining of human pancreas, islet. Immunohistoc...