anti-Human WNT5A antibody for Flow Cytometry

Recommended WNT5A Antibody (supplied by: Log in to see )

Antigen
Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) Antibodies
  • hWNT5A
  • wnt-5A
  • wnt5a-B
  • WNT5A
  • 8030457G12Rik
  • Wnt-5a
  • Wnt family member 5A
  • Wnt family member 5A S homeolog
  • wingless-type MMTV integration site family, member 5a
  • wingless-type MMTV integration site family, member 5A
  • wingless-related MMTV integration site 5A
  • Wnt signaling ligand
  • WNT5A
  • Wnt5a
  • wnt5a.S
  • wnt5a
  • LOC578814
  • wnt5
Epitope
AA 185-213, Center
18
15
14
14
10
7
5
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
153
60
48
19
18
16
3
3
2
1
1
1
1
1
Host
Rabbit
103
58
2
1
Clonality
Polyclonal
Conjugate
This WNT5A antibody is un-conjugated
8
7
6
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
127
65
62
58
17
13
12
5
2
1
1
1
1
Options
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Pubmed
4 references available

Available images

Western Blotting (WB) image for anti-Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (AA 185-213), (Center) antibody (ABIN651869)
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (AA 185-213), (Center) antibody (ABIN651869)
Flow Cytometry (FACS) image for anti-Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (AA 185-213), (Center) antibody (ABIN651869)
Catalog No. ABIN651869
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.952866 ABIN3029634 FACS IHC ELISA WB Rabbit Ig Fraction AA 185-213 Log in to see Polyclonal 0
1 ABIN5534831 FACS IHC (p) WB Rabbit Ig Fraction AA 185-213 Log in to see Polyclonal 0
1 ABIN2570931 ELISA FACS IHC WB Rabbit IgG AA 192-221 Log in to see Polyclonal 0
1 ABIN2387308 FACS IHC ELISA WB Rabbit IgG AA 192-221, Center, Internal Region Log in to see Polyclonal 0
1 ABIN1948762 FACS IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG AA 192-221 Log in to see Polyclonal 0
1 ABIN1948764 FACS IHC ELISA WB Biotin Rabbit IgG AA 192-221 Log in to see Polyclonal 0
1 ABIN1948761 FACS IHC ELISA WB HRP Rabbit IgG AA 192-221 Log in to see Polyclonal 0
1 ABIN1948763 FACS IHC ELISA WB APC Rabbit IgG AA 192-221 Log in to see Polyclonal 0
1 ABIN1948765 FACS IHC ELISA WB FITC Rabbit IgG AA 192-221 Log in to see Polyclonal 0
1 ABIN1948766 FACS IHC ELISA WB PE Rabbit IgG AA 192-221 Log in to see Polyclonal 0
1 ABIN2387305 FACS IHC ELISA WB PE Rabbit IgG AA 192-221, Center, Internal Region Log in to see Polyclonal 0
1 ABIN2387312 FACS IHC ELISA WB APC Rabbit IgG AA 192-221, Center, Internal Region Log in to see Polyclonal 0
1 ABIN2387303 FACS IHC ELISA WB FITC Rabbit IgG AA 192-221, Center, Internal Region Log in to see Polyclonal 0
1 ABIN1809054 FACS IHC (p) WB Rabbit AA 185-213 Log in to see Polyclonal 0
1 ABIN2387311 FACS IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG AA 192-221, Center, Internal Region Log in to see Polyclonal 0
1 ABIN2387313 FACS IHC ELISA WB Biotin Rabbit IgG AA 192-221, Center, Internal Region Log in to see Polyclonal 0
1 ABIN4955788 ELISA FACS IHC WB Rabbit Ig Fraction AA 185-213 Log in to see Polyclonal 0

General

Antigen Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) Antibodies
Epitope AA 185-213, Center
(18), (15), (14), (14), (10), (7), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(153), (60), (48), (19), (18), (16), (3), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(103), (58), (2), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This WNT5A antibody is un-conjugated
(8), (7), (6), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(127), (65), (62), (58), (17), (13), (12), (5), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-WNT5A Antibody

Target Details WNT5A Application Details Handling References for anti-WNT5A antibody (ABIN651869) Images
Specificity This WNT5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-221 amino acids from the Central region of human WNT5A.
Predicted Reactivity Mouse (Murine),Rat (Rattus),Rabbit
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This WNT5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-213 AA from the Central region of human WNT5A.
Clone RB22340
Isotype Ig Fraction

Target Details WNT5A

Product Details anti-WNT5A Antibody Application Details Handling References for anti-WNT5A antibody (ABIN651869) Images back to top
Antigen
Alternative Name WNT5A (WNT5A Antibody Abstract)
Background The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. [provided by RefSeq].
Synonyms: Protein Wnt-5a,WNT5A,
Molecular Weight 42339 DA
Gene ID 7474
NCBI Accession NP_003383
UniProt P41221
Pathways WNT Signaling, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Cell Size, Tube Formation

Application Details

Product Details anti-WNT5A Antibody Target Details WNT5A Handling References for anti-WNT5A antibody (ABIN651869) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-WNT5A Antibody Target Details WNT5A Application Details References for anti-WNT5A antibody (ABIN651869) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-WNT5A antibody (ABIN651869)

Product Details anti-WNT5A Antibody Target Details WNT5A Application Details Handling Images back to top
Background publications

He, Saint-Jeannet, Wang, Nathans, Dawid, Varmus: "A member of the Frizzled protein family mediating axis induction by Wnt-5A." in: Science (New York, N.Y.), Vol. 275, Issue 5306, pp. 1652-4, 1997

Danielson, Pillarisetti, Cohen, Sholehvar, Huebner, Ng, Nicholls, Cheah, Iozzo: "Characterization of the complete genomic structure of the human WNT-5A gene, functional analysis of its promoter, chromosomal mapping, and expression in early human embryogenesis." in: The Journal of biological chemistry, Vol. 270, Issue 52, pp. 31225-34, 1996

Smolich, McMahon, McMahon, Papkoff: "Wnt family proteins are secreted and associated with the cell surface." in: Molecular biology of the cell, Vol. 4, Issue 12, pp. 1267-75, 1994

Clark, Cohen, Eichstetter, Cannizzaro, McPherson, Wasmuth, Iozzo: "Molecular cloning of the human proto-oncogene Wnt-5A and mapping of the gene (WNT5A) to chromosome 3p14-p21." in: Genomics, Vol. 18, Issue 2, pp. 249-60, 1994

Images

Product Details anti-WNT5A Antibody Target Details WNT5A Application Details Handling References for anti-WNT5A antibody (ABIN651869) back to top
Supplier Images
Western Blotting (WB) image for anti-Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (AA 185-213), (Center) antibody (ABIN651869) WNT5A Antibody (Center) (ABIN651869) western blot analysis in Hela cell line lysates ...
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (AA 185-213), (Center) antibody (ABIN651869) WNT5A Antibody (Center) (ABIN651869) immunohistochemistry analysis in formalin fixed ...
Flow Cytometry (FACS) image for anti-Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (AA 185-213), (Center) antibody (ABIN651869) WNT5A Antibody (Center) (ABIN651869) flow cytometric analysis of Hela cells (right hi...