anti-Human WNT8A antibody for Immunohistochemistry

Recommended WNT8A Antibody (supplied by: Log in to see )

Antigen
Wingless-Type MMTV Integration Site Family, Member 8A (WNT8A) Antibodies
  • WNT8A
  • wnt8
  • Xwnt8
  • wnt-8
  • wnt8d
  • Xwnt-8
  • Stra11
  • Wnt-8A
  • Wnt-8D
  • Wnt8d
  • WNT8D
  • wu:fa20e02
  • wu:fe05d07
  • CWNT-8C
  • Wnt8c
  • Wnt family member 8A
  • wingless-type MMTV integration site family, member 8A
  • wingless-type MMTV integration site family, member 8a
  • WNT8A
  • wnt8a
  • Wnt8a
Epitope
Internal Region
15
10
6
4
4
3
1
1
1
1
1
Reactivity
Bat, Human
53
33
16
4
4
3
2
2
1
1
1
1
1
1
1
Host
Rabbit
56
15
2
Clonality
Polyclonal
Conjugate
This WNT8A antibody is un-conjugated
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
32
16
14
13
11
2
1
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Catalog No. ABIN1049484
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.404079 ABIN1049485 IHC IHC (p) Rabbit Internal Region Log in to see Polyclonal 0
8.404079 ABIN213579 IHC IHC (p) Rabbit Internal Region Log in to see Polyclonal 0
8.404079 ABIN6266048 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
8.404079 ABIN2469493 IHC Rabbit Internal Region Log in to see Polyclonal 0
8.404079 ABIN5959994 ELISA IHC Rabbit IgG Log in to see Polyclonal 0
1 ABIN6107517 ELISA IHC Rabbit IgG AA 223-309 Log in to see Polyclonal 0
1 ABIN6038850 ELISA IHC Rabbit IgG Log in to see Polyclonal 0
1 ABIN2390291 IHC Rabbit Log in to see Polyclonal 0
1 ABIN2390292 IHC Rabbit Log in to see Polyclonal 0
1 ABIN2390296 IHC Rabbit Log in to see Polyclonal 0
1 ABIN4366200 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Wingless-Type MMTV Integration Site Family, Member 8A (WNT8A) Antibodies
Epitope Internal Region
(15), (10), (6), (4), (4), (3), (1), (1), (1), (1), (1)
Reactivity Bat, Human
(53), (33), (16), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(56), (15), (2)
Clonality
Polyclonal
Conjugate This WNT8A antibody is un-conjugated
(4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(32), (16), (14), (13), (11), (2), (1)
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Product Details anti-WNT8A Antibody

Target Details WNT8A Application Details Handling Images
Brand IHC-plus™
Specificity Human WNT8A. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Bat (100%) Monkey, Marmoset, Mouse, Panda, Dog, Horse (94%) Rat, Rabbit (89%) Elephant, Bovine (83%).
Purification Immunoaffinity purified
Immunogen Synthetic 18 amino acid peptide from internal region of human WNT8A. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Bat (100%), Monkey, Marmoset, Mouse, Panda, Dog, Horse (94%), Rat, Rabbit (89%), Elephant, Bovine (83%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details WNT8A

Product Details anti-WNT8A Antibody Application Details Handling Images back to top
Antigen
Alternative Name WNT8A (WNT8A Antibody Abstract)
Background Name/Gene ID: WNT8A
Family: WNT

Synonyms: WNT8A, Protein Wnt-8d, WNT8D, Protein Wnt-8a
Gene ID 7478
Pathways WNT Signaling

Application Details

Product Details anti-WNT8A Antibody Target Details WNT8A Handling Images back to top
Application Notes Approved: IHC, IHC-P (10 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-WNT8A Antibody Target Details WNT8A Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-WNT8A Antibody Target Details WNT8A Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family, Member 8A (WNT8A) (Internal Region) antibody (ABIN1049484) Anti-WNT8A antibody ABIN1049484 IHC staining of human breast. Immunohistochemistry o...