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anti-Egl Nine Homolog 3 (C. Elegans) (EGLN3) Antibodies

Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Additionally we are shipping EGLN3 Proteins (11) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
EGLN3 54702 Q62630
EGLN3 112407 Q91UZ4
EGLN3 112399 Q9H6Z9
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Top anti-EGLN3 Antibodies at

Showing 10 out of 138 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Human Rabbit Un-conjugated IHC (p), WB EGLN3 Antibody (C-term) (ABIN658142) western blot analysis in HL-60 cell line lysates (35 µg/lane). This demonstrates the EGLN3 antibody detected the EGLN3 protein (arrow). 400 μL Log in to see 10 to 11 Days
Chicken Rabbit Un-conjugated WB WB Suggested Anti-EGLN3 Antibody Titration: 0.2-1 ug/mlPositive Control: Human brain 100 μL Log in to see 2 to 3 Days
Cow Goat Un-conjugated EIA, WB 0.1 mg Log in to see 6 to 8 Days
Human Mouse Un-conjugated IHC, IHC (p), SimWes, WB Western Blot: EGLN3/PHD3 Antibody (EG188e/d5) [NBP1-30440] - Analysis of HIF Prolyl Hydroxylase 3 in Lane 1: Cobalt chloride treated COS-7 nuclear extracts and Lane 2: Untreated COS-7 nuclear extracts. 0.2 mL Log in to see 5 to 7 Days
Human Rabbit Un-conjugated ChIP, ICC, IF, IHC (p), IP, SimWes, WB Western Blot analysis of human PHD3, using ABIN151769. Samples: HeLa whole cell lysates and MEFs. Simple Western: EGLN3/PHD3 Antibody [ABIN151769] - Simple Western lane view shows a specific band for PHD3/HIF Prolyl Hydroxylase 3 in 0.5 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. 0.1 mL Log in to see 7 to 9 Days
Human Goat Un-conjugated ELISA, WB ABIN302149 (0.1µg/ml) staining of Human Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. 100 μg Log in to see 4 to 5 Days
Human Rabbit Un-conjugated EM, ICC, IF, IHC, IHC (p), IP, WB Detection of Human and Rat PHD3 by Western Blot. Samples: A. Lysate from cells that were transiently transfected with PHD1, PHD2, PHD3 or PHD4 constructs or in vitro translated PHD1, PHD2, PHD3, PHD4. B. Lysate from rat cells before or 9 hours after inducing hypoxia. Antibody: Affinity purified rabbit anti-PHD3 (ABIN151073) use at 1:1,000 (A) or 1:5,000 (B). Detection:Chemiluminence. Western Blot: EGLN3/PHD3 Antibody [ABIN151073] - Detection of PHD3 by Western Blot application in lysate from Rat cells before or 9 hours after inducing hypoxia. Primary antibody PHD3 ABIN151073 was used at a dilution of 1:5000 and blots were developed using chemiluminescence. 0.1 mL Log in to see 7 to 9 Days
Bat Goat Un-conjugated ELISA, WB 100 μg Log in to see 7 to 9 Days
Human Rabbit Un-conjugated ELISA, WB 50 μg Log in to see 2 to 3 Days
Human Goat Un-conjugated ELISA, WB 0.1 mg Log in to see 2 to 3 Days

EGLN3 Antibodies by Reactivity, Application, Clonality and Conjugate

Attributes Applications Host Clonality Conjugate
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Top referenced anti-EGLN3 Antibodies

  1. Human Polyclonal EGLN3 Primary Antibody for EM, ICC - ABIN151073 : Bai, Zeng, Hu, Li, Lin, Shang, Shi: Expression and characteristic of synthetic human epidermal growth factor (hEGF) in transgenic tobacco plants. in Biotechnology letters 2007 (PubMed)
    Show all 35 references for ABIN151073

  2. Human Polyclonal EGLN3 Primary Antibody for ChIP, ICC - ABIN151769 : Mikhaylova, Ignacak, Barankiewicz, Harbaugh, Yi, Maxwell, Schneider, Van Geyte, Carmeliet, Revelo, Wyder, Greis, Meller, Czyzyk-Krzeska: The von Hippel-Lindau tumor suppressor protein and Egl-9-Type proline hydroxylases regulate the large subunit of RNA polymerase II in response to oxidative stress. in Molecular and cellular biology 2008 (PubMed)
    Show all 34 references for ABIN151769

  3. Human Monoclonal EGLN3 Primary Antibody for IHC, IHC (p) - ABIN447479 : Fujita, Gogate, Chiba, Toyama, Shapiro, Risbud: Prolyl hydroxylase 3 (PHD3) modulates catabolic effects of tumor necrosis factor-α (TNF-α) on cells of the nucleus pulposus through co-activation of nuclear factor κB (NF-κB)/p65 signaling. in The Journal of biological chemistry 2012 (PubMed)
    Show all 5 references for ABIN447479

  4. Human Polyclonal EGLN3 Primary Antibody for IHC (p), WB - ABIN658142 : Sato, Sakota, Nakayama: Human PRP19 interacts with prolyl-hydroxylase PHD3 and inhibits cell death in hypoxia. in Experimental cell research 2010 (PubMed)
    Show all 3 references for ABIN658142

  5. Cow (Bovine) Polyclonal EGLN3 Primary Antibody for EIA, WB - ABIN374996 : Nakayama, Gazdoiu, Abraham, Pan, Ronai: Hypoxia-induced assembly of prolyl hydroxylase PHD3 into complexes: implications for its activity and susceptibility for degradation by the E3 ligase Siah2. in The Biochemical journal 2006 (PubMed)

  6. Chicken Polyclonal EGLN3 Primary Antibody for WB - ABIN2784911 : Ginouvès, Ilc, Macías, Pouysségur, Berra: PHDs overactivation during chronic hypoxia "desensitizes" HIFalpha and protects cells from necrosis. in Proceedings of the National Academy of Sciences of the United States of America 2008 (PubMed)

More Antibodies against EGLN3 Interaction Partners

Xenopus laevis Egl Nine Homolog 3 (C. Elegans) (EGLN3) interaction partners

  1. comparative analysis of phd1 (show EGLN2 Antibodies), 2, and 3 expression in Xenopus laevis

Mouse (Murine) Egl Nine Homolog 3 (C. Elegans) (EGLN3) interaction partners

  1. deleting Phd1 (show EGLN2 Antibodies)-3 genes in osteoblasts increased osteoclast formation in vitro and in bone.

  2. PHD3 is an active participant in atherogenesis

  3. Cardiomyocyte-specific transgenic expression of PHD3 impairs the myocardial response to ischemia.

  4. PHD3 protects intestinal epithelial barrier function and reveal a hydroxylase-independent function of PHD3 in stabilizing occludin (show OCLN Antibodies)

  5. depletion of PHD3 leads to increased stabilization of HIF-1alpha (show HIF1A Antibodies) and inhibition of DNA damage response, both of which may contribute to the cardioprotective effect seen with depletion of PHD3.

  6. PHD3 loss sustains cell proliferation through the control of EGFR (show EGFR Antibodies).

  7. combined deletion of Phd2 (show EGLN1 Antibodies) and Phd3 dramatically decreased expression of phospholamban (PLN (show PLN Antibodies)), resulted in sustained activation of calcium/calmodulin (show Calm2 Antibodies)-activated kinase II (CaMKII (show CAMK2G Antibodies)), and sensitized mice to chronic beta-adrenergic stress-induced myocardial injury

  8. PHD1 (show EGLN2 Antibodies) and PHD3 deletions promote angiogenesis in ischemia-injured tissue by increasing HIF1-alpha (show HIF1A Antibodies) stability.

  9. Using conditional PHD3-knockout mice, it was shown that PHD3 affects the production of Angptl2 (show ANGPTL2 Antibodies) and additionally influences the response toward this apoptosis-modulating factor.

  10. ablation of the PHD3 gene resulted in increased angiogenesis and cardiac function after infarction thereby offering a potential target for management of ischemic myocardial disease

Human Egl Nine Homolog 3 (C. Elegans) (EGLN3) interaction partners

  1. demonstrate that downregulation of PHD3 augments metastatic spread in human colorectal cancer and identify MCL-1 (show MCL1 Antibodies) as a novel downstream effector of oxygen sensing

  2. In pancreatic Beta cells, knock-down of PHD3 inhibited glucose-stimulated insulin (show INS Antibodies) secretion.

  3. Loss of PHD3 expression is associated with breast cancer.

  4. The selective efficacy of PZ was further demonstrated at the cellular level by observing inhibition of the PHD3-dependent DNA damage response pathway without stabilization of HIF-1alpha (show HIF1A Antibodies).

  5. The enhanced expression of PHD3 might likely contribute to the poor neovascularization and affect the biological characterization in PDAC cancer cells

  6. The data demonstrates that PHD3 can drive cell cycle entry at the G1/S transition through decreasing the half-life of p27 (show PAK2 Antibodies) that occurs by attenuating p27S10 phosphorylation.

  7. PHD3 controls EGFR (show EGFR Antibodies) activity by acting as a scaffolding protein that associates with the endocytic adaptor Eps15 (show EPS15 Antibodies) and promotes the internalization of EGFR (show EGFR Antibodies).

  8. PHD3 loss sustains cell proliferation through the control of EGFR (show EGFR Antibodies).

  9. PHD3 SUMOylation occurs at a cluster of four lysines at the C-terminal end of the protein. Furthermore, PHD3 SUMOylation by SUMO2 (show SUMO2 Antibodies) or SUMO3 (show SUMO3 Antibodies) contributes to PHD3-mediated repression of HIF1 (show HIF1A Antibodies)-dependent transcriptional activity.

  10. HIFPH3 expression in human non-small cell lung cancer lesions is significantly higher than that in para-cancerous and normal lung tissues and is positively associated with lymph node metastasis and microvessel density.

EGLN3 Antigen Profile

Protein Summary

Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. ELGN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM2 in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Regulator of cardiomyocyte and neuronal apoptosis. In cardiomyocytes, inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex. In neurones, has a NGF-induced proapoptotic effect, probably through regulating CASP3 activity. Also essential for hypoxic regulation of neutrophilic inflammation.

Gene names and symbols associated with EGLN3

  • egl nine homolog 3 (C. elegans) (egln3) antibody
  • egl nine homolog 3 (egln3) antibody
  • egl nine homolog 3 (LOC100354210) antibody
  • egl nine homolog 3 (C. elegans) (EGLN3) antibody
  • egl nine homolog 3 (C. elegans) (LOC100219039) antibody
  • EGL nine homolog 3 (C. elegans) (Egln3) antibody
  • egl-9 family hypoxia-inducible factor 3 (EGLN3) antibody
  • 2610021G09Rik antibody
  • AI505553 antibody
  • AI648162 antibody
  • EGLN3 antibody
  • Hif-p4h-3 antibody
  • HIFP4H3 antibody
  • HIFPH3 antibody
  • PHD-3 antibody
  • Phd3 antibody
  • SM-20 antibody
  • wu:fj78a08 antibody
  • zgc:77019 antibody

Protein level used designations for EGLN3

Hif-p4h-3 , egl nine homolog 3 , phd3 , egl nine homolog 3 (C. elegans) , HIF-PH3 , HIF-prolyl hydroxylase 3 , HPH-3 , egl nine homolog 3, mitochondrial , factor-responsive smooth muscle protein , hypoxia-inducible factor prolyl hydroxylase 3 , prolyl hydroxylase domain-containing protein 3 , HIF prolyl hydroxylase 3 , HPH-1 , egl nine-like protein 3 isoform

406602 Danio rerio
100145602 Xenopus (Silurana) tropicalis
100354210 Oryctolagus cuniculus
452850 Pan troglodytes
480286 Canis lupus familiaris
100219039 Taeniopygia guttata
100127282 Xenopus laevis
54702 Rattus norvegicus
423316 Gallus gallus
535578 Bos taurus
112407 Mus musculus
112399 Homo sapiens
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