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Primary Antibodies

Primary antibodies are immunoglobulins that bind to a specific antigen of interest. The antigen can be for example a protein, a peptide, a small molecule. A high-quality primary antibody recognizes and binds with high affinity and specificity to purify, detect, and measure the antigen. Using different hosts, such as mouse, rabbit, rat or chicken, primary antibodies are available as polyclonal or monoclonal antibodies, depending on the application. Primary antibodies for commonly studied targets are also available in conjugation with fluorescent dyes or biotin, PE and other molecules.

At antibodies-online.com you will find high-quality primary antibodies with diverse validation data for a large number of targets, reactivities and conjugates.

Type in your target and browse our range of primary antibodies!

Featured primary antibodies categories

Monoclonal Antibodies

Learn about our thousands of mouse, rat, rabbit, and other species of monoclonal primary antibodies.

Polyclonal Antibodies

Learn about our thousands of rabbit, goat, chicken, and other species of polyclonal primary antibodies.

Recombinant Antibodies

Learn about our high quality recombinant antibodies for a wide variety of targets.

CUT&RUN Antibodies

Antibodies validated for CUT&RUN assays, e.g. against histones, transcription factors etc.

Epigenetics Antibodies

Reliable antibodies for Epigenetics research. With high quality validation data.

Cell Markers Antibodies

Antibodies against the cell markers most commonly used in research.

Popular Primary Antibodies

Product
Reactivity
Application
Validations
Cat. No.
Quantity
Datasheet
ReactivityDiscosoma
ApplicationELISA, FACS, IF, IHC, IHC (fro), IHC (p), IP, WB
Validations
  • (349)
  • collections(11)
  • (1)
Cat. No.ABIN129578
Quantity100 μL
ReactivityCow, Human, Mammalian, Mouse, Rat
ApplicationELISA, IHC, IP, WB
Validations
  • (62)
  • collections(41)
  • (1)
Cat. No.ABIN5596819
Quantity100 μg
Reactivity
ApplicationIP, ELISA, WB
Validations
  • (20)
  • collections(6)
  • (2)
Cat. No.ABIN99294
Quantity250 μg
ReactivityHuman, Mouse, Rat
ApplicationCUT&RUN, FACS, ICC, IF, IHC (p), IP, WB
Validations
  • (11)
  • collections(15)
  • (1)
Cat. No.ABIN2855074
Quantity100 μL
ReactivityHuman, Mouse, Rat
ApplicationIHC (fro), IHC (p), IHC, WB
Validations
  • (13)
  • collections(17)
  • (1)
Cat. No.ABIN2857032
Quantity100 μL
ReactivityHuman, Mouse, Rat
ApplicationChIP, CUT&RUN, ICC, IF, IHC (p), IP, WB
Validations
  • (6)
  • collections(14)
  • (1)
Cat. No.ABIN2855042
Quantity100 μL
ReactivityAvian, Cat, Chicken, Cow, Dog, Donkey, Goat, Guinea Pig, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep
ApplicationIF, WB
Validations
  • (5)
  • collections(3)
  • (3)
Cat. No.ABIN1440014
Quantity600 μg
ReactivityHuman, Mouse
ApplicationIHC, ELISA, WB
Validations
  • (1)
  • collections(7)
  • (1)
Cat. No.ABIN95037
Quantity100 μg
ReactivityHuman
ApplicationIHC, WB
Validations
  • (23)
  • collections(6)
  • (2)
Cat. No.ABIN2777474
Quantity100 μL
ReactivityHorse, Human, Mouse, Rabbit, Rat
ApplicationELISA, ICC, IF (cc), IF (p), IHC (fro), IHC (p), WB
Validations
  • (20)
  • collections(6)
  • (1)
Cat. No.ABIN677318
Quantity100 μL

antibodies-online.com supplies you with antibodies for more than 60,000 targets, for more than 100 different applications and methods, a wide variety of conjugates, reactivities etc. In case you need assistance with finding the right product our team of PhD biologists is looking forward to your request via phone or email to support you in your research work.

Browse our Primary Antibodies by Application

We offer antibodies for more than 100 applications. Below are some of the most requested. If you do not find the method you are looking for, please use our search and filter out the desired products quickly and easily, or contact our customer service.

We support you with antibodies validated for many other applications such as FLISA, Immunocytochemistry, and more. Use our search to find the right product for you.

Browse our Primary Antibodies by Conjugate

On antibodies-online.com you will find a wide selection of conjugated primaries. Some of the most important conjugates are listed below. By clicking on a link you will get to the corresponding products.

We support you with a wide range of conjugates. Please use our search function or contact our team of biologists if you need help finding your desires product.

Reagents and Tools related to Primary Antibodies

On antibodies-online.com you will find a lot of other tools and reagents for your daily work. Some important categories are shown below.

Resources

Antibody FAQ

What is an antibody?

Antibodies are proteins produced in vertebrates in response to certain substances. Antibodies are an important element of the so-called humoral (antibody-mediated) immunity. They belong to the gamma globulin fraction and are also called immunoglobulins (Ig).

Antibodies are produced by the body when B cells come in contact with a matching antigen. As a result, the B cell is activated and differentiates into a plasma cell that secretes large amounts of antibody. These antibodies are able to specifically bind the antigen which has caused the differentiation of the B cell.

How are antibodies structured?

Antibodies have a common basic structure: each consists of two heavy and two light identical polypeptide chains, linked via disulfide bridges. The heavy and light chains include constant and variable regions. The variable regions of both chains determine the binding domain (paratope) for a specific antigenic determinant (epitope). The epitope is the part of the antigen that is recognized by the antibody. In addition, the constant regions of the heavy chains determine the immunoglobulin class to which the antibody belongs (IgM, IgG, IgD, IgA or IgE). The immunoglobulin class is also called isotype. The various antibody classes are found in different compartments of the body. For example, IgA is present in the saliva while IgG and IgM are found in the blood. In addition, membrane-bound antibodies are also found (e.g.: IgE on mast cells or IgD on B lymphocytes).

What is the function of antibodies?

In the course of an immune response, the antibodies perform various functions. They capture and block invading antigens so that they cannot exert their harmful effect, or they prevent the antigen’s interaction with body cells (e.g. to prevent the penetration of viruses and bacteria into body cells).

Antibodies also opsonize pathogens. In this case the antibody constitutes a marker, e.g. for phagocytes. The phagocytes interact with the constant regions of the antibody in contact and are induced to uptake and digest the pathogen (e.g., a bacterium).

Antibodies may also bind to body cells, thereby causing the so-called NK cells (natural killer cells) to selectively kill the cell. This is useful when a cell is infected with a pathogen (e.g., a virus). Other important functions include the activation of various cell types of the immune system and the complement system.

What are antibodies used for?

Antibodies are used in medicine for diagnostic and therapeutic purposes (e.g. serological tests, diagnosis and follow-up of infectious diseases, passive immunization, etc.). They also offer many possible uses (ELISA, FACS, Western blots, immunohistochemistry, etc.) for research purposes. For these purposes, polyclonal antibodies, monoclonal antibodies or recombinant antibodies are used.

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