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ELISA Kits

Compare and order fully validated high-quality ELISA Kits (enzyme-linked immunosorbent assay kits). On antibodies-online you can find a wide range of reliable kits to quickly and easily detect or quantify proteins in biological samples.

Browse our range of ELISA kits!

Quick links to popular ELISA kit targets:

What is an ELISA Kit?

ELISA kits are ready-to-use immunoassay kits that contain all the reagents and materials necessary to perform an ELISA. Individual components are already tested, and have been proven effective and compatible with each other. Together this safes a lot of time as no additional preparations have to be made. The enclosed step-by-step instructions allow to perform the assay without deep knowledge of the topic. Last but not least the cost-per-sample is generally lower than for de novo assay generation.

What are the advantages of using ELISA kits?

In order to perform an ELISA you need several materials. A pre-coated 96-well strip plate several diluents, a stop solution, a standard to test against, a substrate in order to generate the color response detection reagents and a wash buffer. This saves time when compared to gathering all materials and mix the solutions prior to each assay. ELISA kits are a convenient approach to perform your desired ELISA. The kit comes in a box containing all of the necessary reagents at once – no need to buy anything extra.

Save working time
The kit comes in a box containing all of the necessary reagents.
Ensure compatibility
Components are tested and have been proven compatible with each-other.
Avoid quality issues
Reagents of reliable quality: All products are covered by our money-back guarantee.
Save money
The cost-per-sample is generally lower than for de novo assay generation.

Featured Products with comprehensive Validation Data

Product
Sample Type
Analytical Method
Cat. No.
Validations
Quantity
Sample TypeCell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Analytical MethodQuantitative Sandwich ELISA
Cat. No.ABIN364986
Validations
  • (10)
  • collections(2)
  • (1)
Quantity96 tests
Sample TypeCell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Analytical MethodQuantitative Sandwich ELISA
Cat. No.ABIN364989
Validations
  • (1)
  • collections(2)
  • (1)
Quantity96 tests
Sample TypeCell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum, Tissue Homogenate
Analytical MethodQuantitative Sandwich ELISA
Cat. No.ABIN411259
Validations
  • (12)
  • collections(1)
  • (1)
Quantity96 tests
Sample TypeCell Culture Supernatant, Cerebrospinal Fluid, Plasma, Saliva, Serum, Tissue Homogenate, Urine
Analytical MethodQuantitative Sandwich ELISA
Cat. No.ABIN365052
Validations
  • (5)
  • collections(1)
  • (1)
Quantity96 tests
Sample TypeCell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Analytical MethodQuantitative Sandwich ELISA
Cat. No.ABIN365803
Validations
  • (2)
  • collections(1)
  • (1)
Quantity96 tests
Sample TypePlasma, Serum, Tissue Homogenate
Analytical MethodQuantitative Sandwich ELISA
Cat. No.ABIN365957
Validations
  • collections(1)
  • (1)
Quantity96 tests
Sample TypeCell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Analytical MethodQuantitative Sandwich ELISA
Cat. No.ABIN1136417
Validations
  • (10)
  • (1)
Quantity96 tests
Sample TypeCell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Analytical MethodQuantitative Sandwich ELISA
Cat. No.ABIN411293
Validations
  • (31)
  • collections(1)
  • (1)
Quantity96 tests
Sample TypeCell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Analytical MethodQuantitative Sandwich ELISA
Cat. No.ABIN365163
Validations
  • (30)
  • collections(2)
  • (1)
Quantity96 tests
Sample TypePlasma, Serum, Tissue Homogenate
Analytical MethodQuantitative Sandwich ELISA
Cat. No.ABIN365165
Validations
  • (6)
  • collections(1)
  • (1)
Quantity96 tests
Sample TypeCell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Analytical MethodQuantitative Competition ELISA
Cat. No.ABIN6574100
Validations
  • (21)
  • collections(3)
  • (1)
Quantity96 tests
Sample TypePlasma, Serum, Tissue Homogenate
Analytical MethodQuantitative Sandwich ELISA
Cat. No.ABIN365639
Validations
  • (2)
  • collections(1)
  • (1)
Quantity96 tests
Sample TypeSerum
Analytical MethodQuantitative Sandwich ELISA
Cat. No.ABIN367216
Validations
  • (2)
  • collections(1)
  • (1)
Quantity96 tests
Sample TypePlasma, Serum, Tissue Homogenate
Analytical MethodQuantitative Sandwich ELISA
Cat. No.ABIN365420
Validations
  • (1)
  • collections(2)
  • (1)
Quantity96 tests
Sample TypeCell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Analytical MethodQuantitative Sandwich ELISA
Cat. No.ABIN411361
Validations
  • (157)
  • collections(1)
  • (1)
Quantity96 tests

antibodies-online supplies you with ELISA kits for more than 2,000 different targets. You can search for the ELISA kit you need by target name, species, detection method, sample types, validation data. In case you need assistance, our customer service team of PhD biologists is looking forward to your request via phone or email to support you in your research work.

COVID-19 / SARS-CoV-2 ELISA Kits

We offer High-quality ELISA Kits:

ELISA Kit Category Description
Cell ELISA (In-Cell ELISA, Cell-based ELISA or In-Cell Western) is a quantitative immunocytochemistry method to measure protein levels or posttranslational modifications of cultured cells. Representing a simple, sensitive method for intracellular protein quantification in whole cells. Cell ELISA are perfectly suitable to monitor effects of stimulation and/or inhibition of treatments, compounds (e.g. siRNA, chemicals) on target molecules. Click the link to find all available products.
DNA-Binding ELISA Kits allow scientist to study the direct interaction between proteins and DNA. With this method active transcription factors in nuclear or cell lysates can easily be detected and qualitative determined. Methods such as electrophoretic mobility shift assays (EMSAs), chromatin immunoprecipitation (ChIP), Western Blotting (WB) and reporter gene fusion are often time-consuming and complicated. The enzyme immunoassay was developed for rapid detection and quantitation without the need of complex sample preparation or harmful radioactive labelling. The sensitivity is comparable or higher to more complicated methods. Click the link to discover our portfolio of DNA-binding ELISA kits.
antibodies-online.com offers you a variety of ELISA kits with uncoated plates. Like other ELISA kits they come with detailed information and protocols and are easy to use. They just require an additional coating step rewarding you with competitive prices.
In an indirect ELISA the primary antibody used to detect the analyte is not conjugated to an enzyme. Instead, an enzyme-conjugated secondary antibody which is reactive against the primary antibody is added to the mixture. Use of a secondary antibody has many of the same advantages in an ELISA as in other proteomics methods. In addition to not requiring the researcher to undergo the sometimes arduous process of conjugating a primary antibody, a secondary antibody can help to amplify a weak signal, allowing a previously undetectable signal to be visualized and lowering the detection threshold of the assay in question. Follow the link to search our portfolio of indirect ELISA kits.
The bridging ELISA is a special case of a sandwich ELISA in which a dimeric or oligomeric antigen (usually an antibody in a sample) is detected by a capture and a detection antibody. The antigen bridges the two specific antibodies. Bridging ELISAs are commonly used for the detection of ADAb in clinical trials, among other applications. Find our products by clicking the link.
Most commercially available ELISAs are sandwich format assays. Sandwich ELISAs typically require the use of matched antibody pairs (capture and the detection antibody), where each antibody is specific for a different, epitope of the antigen molecule. The capture antibody often is a monoclonal antibody as they only bind a single epitope on an antigen. This increases the specificity and reduces background noises as well. A polyclonal is often used as the capture antibody to pull down as much of the antigen as possible. Sandwich ELISA can be direct or indirect depending on whether the detection antibody is labeled or unlabeled. Click the link to search our portfolio of ELISA kits for sandwich ELISA.
In a competitive format assay, as antigen concentration in a sample increases, signal intensity decreases. The central concept behind a competitive ELISA is that a larger quantity of analyte in a sample results in fewer free antibodies in solution and by extension a smaller number of labeled antibodies bound to the standard on the plate. A primary antibody (unlabeled) is incubated with sample antigen. They are then added to 96-well plates which are pre-coated with the same antigen, followed by a washing step. Antigen quantity in the sample determines the amount antibody binding on the well. The secondary antibody that is specific to the primary antibody and conjugated with an enzyme is added. A substrate is added, and remaining enzymes elicit a chromogenic or fluorescent signal. The competitive assay is an appealing option if no suitable antibody pair can be identified for sandwich ELISA, or if the analyte in question is too small to permit binding of both a capture and detection antibody.

When to build an assay, when to buy an ELISA kit?

Once you’ve decided that you’d like to use an ELISA, you need to decide whether you’ll use a commercially available ELISA kit, or try to compile an assay on your own from scratch. There are a few reasons that one might want to try to generate an assay de novo rather than purchasing a pre-made solution. If you need to fine tune your assay outside the specifications in a commercially available kit, if you need tight control over the specific nature of the components that go in to your assay, or if you need to use a specific or custom antibody in your assay you might want to consider investing the time and effort necessary to generate an assay de novo.

However, if you’re not limited to the criteria above, you might want to consider purchasing a commercially available assay for simplicity’s sake. You should consider purchasing a pre-made kit if you want to:

  • Save time: Optimization of custom ELISA is very time consuming. Even for an experienced scientist with compatible reagents, a new assay will generally require hours or days of optimization before it is ready for use. That’s all time that you could be using to collect, analyze, interpret, and publish your data. Carefully consider the value of your time, and the time of the technicians and students who will be building and performing the assay.
  • Ensure reagent compatibility: A pre-made ELISA kit is guaranteed to contain reagents that are compatible with each other. When compiling your own assay, even if you choose high quality reagents you may accidentally stumble upon incompatible products (e.g. two antibodies that recognize a similar epitope and block each other due to steric hindrance).
  • Avoid frustrating quality-control issues: When generating an assay from scratch, you'll likely be sourcing different reagents from several different places. And after spending all the time necessary to track down each of the various reagents you need, even a single bad buffer could ruin your experiment, requiring countless hours of troubleshooting to trace the source of the problem. By contrast, every pre-made ELISA kit from antibodies-online is covered by our money back guarantee. In the unlikely event that you do experience a problem, our technical support staff will handle the troubleshooting.
  • Save money: While it may be tempting to try to piece together your own ELISA to save a few dollars, most researchers find that the cost-benefit analysis doesn't actually add up in the end. After considering all of the different components that must be purchased to generate an assay de novo it is often just as cost effective to buy a pre-made kit.

Need Help? Call our PhD Customer Support!

  • We help you with finding the right product for your research.
  • We offer reliable antibodies, kits, proteins, lysates for your research.
  • Contact us via email or phone: (877) 302 8632 (US) or +49 241 95 163 153 (International)

ELISA Kits - Frequently asked Questions

Can I modify the protocol?

ELISA kits have been optimized to provide the best possible results. If you modify the format or protocol, it may give inaccurate and wrong results.

How should I store my samples?

For best results using fresh samples is recommended. Alternatively samples should be stored in a freezer at -20 °C, -80 °C, or in liquid nitrogen.

Can components from different kits be used?

It is not recommended to use components other than those included in the ELISA kit. The components of a kit are precisely matched to each other and confirmed in their efficacy.

How many samples can I test on one ELISA?

On each 96-well plate an 8-point standard curve with duplicate wells is performed. The remaining wells can be used for samples. If the entire plate is used in an experiment, the remaining 80 wells can accommodate 40 duplicate samples.

Can antibodies-online ELISA kits be used for diagnostic or therapeutic procedures?

No, the ELISA kits are for research use only.

Do you also offer ELISA kits with uncoated plates?

Yes, we do. You can find them using our search filter "plate".

Do your ELISA kits work for cell and tissue lysates?

The sample types for which an ELISA kit has been proven to work are indicated in the respective product description.

What is the "Independent Validation Initiative"?

Our Independent Validation Initiative addresses the call for more reproducibility in the proteomics market by providing well documented, independently generated, and scientifically sound data for our ELISA kits, and other products from our catalog. Please let us know, if you are interested in validating a specific product. In return for your data, you will receive your money back for the product.

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