Anti-Rabbit Secondary Antibodies

Choose from a handpicked selection of anti-rabbit secondary antibodies with enzymatic conjugates like AP, Biotin or HRP or fluorescent conjugates including FITC, APC and DyLight and Atto dye families. Browse our offer for a variety of different host, isotypes or binding specificities.

Anti-Rabbit Secondary Antibodies with Enzymatic Conjugates

Secondary antibodies linked with enzymes such as HRP and alkaline phosphatase AP are commonly utilized in applications such as ELISA or Western blotting. Both can be detected using colorimetric or chemiluminescent readout.

Anti-Rabbit Secondary Antibodies with Fluorescent Conjugates

Secondary antibodies conjugated to fluorescent proteins, eg FITC, TRITC, APC or PE or commercial dye families excel in flow cytometry or immunofluorescence experiments. For example the FITC conjugated Goat Anti-Rabbit IgG. DyLight flurorescent conjugates are an excellent alternative to IRDye800CW or IRDye600CW, they offer comparable excitation and emission spectra and are claimed to be more photostable.

Goat is the predominant host species to produce polyclonal anti-rabbit secondary antibodies. Your experiments require a different host species? antibodies-online offers anti-rabbit secondary antibodies raised in variety of species like donkey, sheep or chicken.

Polyclonal primary antibodies raised in rabbit are generally IgG isotypes. Therefore you will need a secondary antibody that is specific to rabbit IgG (anti-rabbit IgG) which reacts with the heavy and light chains of IgG. You are working with primary rabbit monoclonals and need a specific isotype for your secondary? Browse our selection below.

Anti-Rabbit Secondary Antibodies Fragments

Secondary antibody fragments offer special options in detection of primary antibodies. Alpaca single domain antibodies for example contain only heavy chains and recognize Fab and Fc fragments of rabbit IgG. F(ab')2 fragments on the other hand lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. The small size enables better tissue penetration, antigen access and decreases the distance between the epitope and label leading to better signal generation in IHC and higher resolution images.

Anti-Rabbit Secondary Antibodies for Specific Binding Sites

Anti-IgG, Fc fragment specific antibodies may be used to detect native IgG primary antibodies without binding to the 25 kDa band of reduced and denatured IgG light chains on Western blots.

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