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FACS Antibodies

Fluorescence-activated cell sorting (FACS) is a specialized type of flow cytometry. It sorts a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell and its attached antibodies.

Important Targets

Arginase 1 Antibodies

CASP8 Antibodies

CCR2 Antibodies

CD14 Antibodies

FOXP3 Antibodies

IL17 Antibodies

Lamin B1 Antibodies

All Flow Cytometry Antibodies

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85,958 results:

RFP antibody

RFP Reactivity: Discosoma WB, ELISA, IF, IP, IHC, FACS, IHC (fro), IHC (p) Host: Rabbit Polyclonal unconjugated

Immunofluorescence validation image for anti-Red Fluorescent Protein (RFP) antibody (ABIN1043867)

Western Blot of Rabbit anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunohistochemistry of Anti-RFP Antibody.

Western Blot of Rabbit Anti-RFP MW hu, ms, rt Antibody.

Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody.

Western Blot of Rabbit anti-RFP Antibody.

ELISA Results of Polyclonal Rabbit Anti-RFP Antibody tested against purified RFP protein.

Western Blot of RFP Antibody Pre-Absorbed.

12 images

355 references

Independent Validation

1 validation

Catalog No. ABIN129578

Datasheet as PDF

Caspase 8 antibody (AA 411-482)

CASP8 Reactivity: Human, Mouse, Rat, Monkey, Pig, Sheep WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Caspase 8 (CASP8) (AA 411-482) antibody (ABIN724205)

Image provided by the Independent Validation Program (badge number 29759).

Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-Caspase-8 Polyclonal Antibody, Unconjugated (ABIN724205) 1:200 followed by conjugation to the secondary antibody and DAB staining

L1 mouse liver lysates L2 mouse spleen lysates probed with Anti Caspase 8 Polyclonal Antibody, Unconjugated (ABIN724205) at 1:200 overnight at 4 °C.

Image provided by One World Lab validation program.

Image provided by One World Lab validation program.

6 images

15 references

Independent Validation

1 validation

Catalog No. ABIN724205

Datasheet as PDF

CTNNB1 antibody (N-Term)

KO Validated CTNNB1 Reactivity: Human WB, IHC, IF, IHC (p), FACS, IP, ICC, IHC (fro), ChIP, IHC (wm) Host: Rabbit Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) (N-Term) antibody (ABIN2855042)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) (N-Term) antibody (ABIN2855042)

WB Image beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein by western blot analysis.

WB Image Sample (30 ug of whole cell lysate) A: HeLa 7.

IHC-P Image beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse urinary bladder by immunohistochemical analysis.

WB Image Sample (50 ug of whole cell lysate) A: mouse brain 7.

IHC-P Image beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis.

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using beta Catenin, antibody (Green) at 1:200 dilution.

WB Image beta Catenin antibody [N1N2-2], N-term detects CTNNB1 protein by Western blot analysis.

IP Image Immunoprecipitation of beta Catenin protein from HeLa whole cell extracts using 5 μg of beta Catenin antibody, Western blot analysis was performed using beta Catenin antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

IHC-P Image beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis.

IHC-P Image Immunohistochemical analysis of paraffin-embedded U373 xenograft, using beta Catenin, antibody at 1:500 dilution.

Effects of MCME on migration-related proteins in CL1-0 and CL1-5 cells.

Expression analysis of Wnt pathway genes in human OS cellsWestern blot analysis of active β-catenin, total β-catenin and β-actin proteins in human OS cell lines (143B, Saos-2, SJSA-1 , U-2 OS, MG-63) with human breast cancer cell high-level-expression controls (MCF7, MDA-MB-231) and additional human mesenchymal stem cell low-level-expression controls (hMSC).

SGSM2 silencing affected T47D cell adhesion and cell migration.

Interaction between SGSM2 and E-cadherin was verified by co-immunoprecipitation and immunofluorescence staining assays.

16 images

8 references

Independent Validation

1 validation

Catalog No. ABIN2855042

Datasheet as PDF

Iba1 antibody (C-Term)

IBA1 Reactivity: Human WB, IHC, IF, ICC, IHC (p), FACS, IHC (fro), IHC (ff) Host: Rabbit Polyclonal unconjugated

Immunofluorescence validation image for anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (C-Term) antibody (ABIN2857032)

IHC-P Image Iba1 antibody detects Iba1 protein on mouse fore brain by immunohistochemical analysis.

IHC-P Image Iba1 antibody detects Iba1 protein on rat hind brain by immunohistochemical analysis.

WB Image Iba1 antibody detects Iba1 protein by western blot analysis.

IHC-P Image Iba1 detects Iba1 protein at cytosol on mouse brain tissue by immunohistochemical analysis.

IHC-P Image Iba1 antibody detects Iba1 protein at cytoplasm on human colon carcinoma stroma by immunohistochemical analysis.

WB Image Sample (30 ug of whole cell lysate) A: Molt-4 , 15% SDS PAGE AIF1 antibody antibody diluted at 1:1000

IHC-P Image Immunohistochemical analysis of paraffin-embedded human hepatoma, using Iba1, antibody at 1:500 dilution.

WB Image Iba1 antibody detects Iba1 protein by Western blot analysis.

ASI administration deactivated microglial activation and reduced inflammatory responses in spinal cords of EAE mice.

Neuropathology in the central nervous system of Fuca1-deficient mice.

W20 attenuated neuropathology and gliosis in the brains of A53T α-synuclein mice.

W20 reduced neuropathology and neuroinflammation in BACHD mice.

Absence of neuroinflammation in RNase H2ΔGFAP mice.

Effects of NADPH oxidase subunit deletion on plasma TNFα levels and microglial activation after LPS injection.

DPI post-treatment attenuated microglia activation in brain after LPS injection.

Expression of microglial marker protein characteristic for the homeostatic or disease-associated stateAImmunoblot analysis of ApoE, CLEC7A, TREM2, and CD68 in lysates of acutely isolated microglia from Grn-/- and Grn+/+ mice (9 months of age, n = 3 per genotype, female).

Development of myeloma with associated spinal glial cell activation.

18 images

13 references

Independent Validation

1 validation

Catalog No. ABIN2857032

Datasheet as PDF

Dendra 2 antibody

Reactivity: Coral WB, FACS, ICC Host: Rabbit Polyclonal unconjugated

Immunocytochemistry validation image for anti-Dendra 2 antibody (ABIN361314)

Localization of Notch::Dendracrispr (anti-Dendra (ABIN361314), green), Histone2B::RFP expressed under the neur promoter (blue) and Delta (anti-Delta, red) in wild-type SOP daughter cells (D, n=9) and in strat SOP daughter cells expressing pnrGAL4>AP-47dsRNA (E, n=20).

Single optical section images showing Dendra2-NTR (green), Tomato (red), Bhmt (blue), and DAPI (gray) expression in regenerating livers of the WT and lvl at R48h with control (top panel) and Mtz treatment (bottom panel).

WB: line1 – Plum protein 27 kDa, line2 – E.

Localization of NiDendra (anti-Dendra, green), Histone2B::RFP expressed under the neur promoter (blue) and Delta (anti-Delta, red) in wild-type SOP daughter cells and in strat SOP daughter cells expressing pnr-GAL4>AP-47dsRNA.

5 images

16 references

Independent Validation

1 validation

Catalog No. ABIN361314

Datasheet as PDF

Occludin antibody (AA 431-522)

OCLN Reactivity: Human, Mouse, Pig WB, ELISA, FACS, IF (cc), IF (p), IHC (p) Host: Rabbit Polyclonal unconjugated

Immunofluorescence validation image for anti-Occludin (OCLN) (AA 431-522) antibody (ABIN687337)

Human A549 lysates probed with Rabbit Anti-Occludin Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

HQT regulates epithelial proliferation in the colonic mucosa of mice with DSS-induced acute and chronic colitis.

Formalin-fixed and mouse kidney tissue labeled with Anti-Occludin Polyclonal Antibody, Unconjugated (ABIN687337) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse embryo tissue labeled with Anti-Occludin Polyclonal Antibody, Unconjugated (ABIN687337) at 1:100 followed by conjugation to the secondary antibody and DAB staining

Independently Validated Antibody, image provided by Science Direct, badge number 029632:Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-OCLN/Occludin Polyclonal Antibody, Unconjugated (ABIN687337) at 1:200 followed by conjugation to the secondary antibody and DAPI staining

Formalin-fixed and paraffin embedded mouse embryo tissue labeled with Anti-Occludin Polyclonal Antibody, Unconjugated at 1:100 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and mouse kidney tissue labeled with Anti-Occludin Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Tight junction proteins distribution of IPEC-J2 cells after 24 h incubation of β-conglycinin.

Western blot of tight junction proteins of IPEC-J2 cells after 24 h incubation of β-conglycinin.

10 images

5 references

Independent Validation

1 validation

Catalog No. ABIN687337

Datasheet as PDF

DVL1 antibody (AA 21-100)

DVL1 Reactivity: Human, Mouse, Rat WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Immunohistochemistry validation image for anti-Dishevelled Segment Polarity Protein 1 (DVL1) (AA 21-100) antibody (ABIN670671)

Immunohistochemistry validation image for anti-Dishevelled Segment Polarity Protein 1 (DVL1) (AA 21-100) antibody (ABIN670671)

Immunohistochemistry validation image for anti-Dishevelled Segment Polarity Protein 1 (DVL1) (AA 21-100) antibody (ABIN670671)

Immunohistochemistry validation image for anti-Dishevelled Segment Polarity Protein 1 (DVL1) (AA 21-100) antibody (ABIN670671)

Images provided the Independent Validation Program (badge number 029657)Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-DVL1 Polyclonal Antibody (ABIN670671) at 1:250 overnight at room temperature followed by conjugation to secondary antibody.

Lane 1: rat brain lysates Lane 2: rat lung lysates probed with Anti DVL1/Dishevelled Polyclonal Antibody, Unconjugated (ABIN670671) at 1:200 in 4 °C.

6 images

1 reference

Independent Validation

1 validation

Catalog No. ABIN670671

Datasheet as PDF

TJP1 antibody (AA 1551-1702)

TJP1 Reactivity: Human, Mouse, Rat, Pig, Cow WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

Immunohistochemistry validation image for anti-Tight Junction Protein 1 (TJP1) (AA 1551-1702) antibody (ABIN675024)

Immunohistochemistry validation image for anti-Tight Junction Protein 1 (TJP1) (AA 1551-1702) antibody (ABIN675024)

Immunohistochemistry validation image for anti-Tight Junction Protein 1 (TJP1) (AA 1551-1702) antibody (ABIN675024)

Immunohistochemistry validation image for anti-Tight Junction Protein 1 (TJP1) (AA 1551-1702) antibody (ABIN675024)

Immunohistochemistry validation image for anti-Tight Junction Protein 1 (TJP1) (AA 1551-1702) antibody (ABIN675024)

Immunohistochemistry validation image for anti-Tight Junction Protein 1 (TJP1) (AA 1551-1702) antibody (ABIN675024)

Image kindly submitted by Kamlesh Gupta as part of the free sample program.

HQT regulates epithelial proliferation in the colonic mucosa of mice with DSS-induced acute and chronic colitis.

Formalin-fixed and paraffin embedded rat brain labeled with Anti- ZO-1 Polyclonal Antibody, Unconjugated (ABIN675024) followed by conjugation to the secondary antibody and DAB staining

Image kindly submitted by Kamlesh Gupta as part of the free sample program.

Image was kindly submitted by Sriram Srivatsa from Medical University of Vienna.

Tight junction proteins distribution of IPEC-J2 cells after 24 h incubation of β-conglycinin.

Western blot of tight junction proteins of IPEC-J2 cells after 24 h incubation of β-conglycinin.

13 images

7 references

Independent Validation

1 validation

Catalog No. ABIN675024

Datasheet as PDF

Elastin antibody (AA 681-786)

ELN Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p), FACS Host: Rabbit Polyclonal unconjugated

Immunohistochemistry validation image for anti-Elastin (ELN) (AA 681-786) antibody (ABIN734003)

Mouse lung lysates probed with Elastin Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

Formalin-fixed and paraffin embedded human glioma labeled with Anti-Elastin Polyclonal Antibody, Unconjugated (ABIN734003) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

L1 mouse intestine lysates L2 rat lung lysates probed with Anti Alpha Elastin Polyclonal Antibody, Unconjugated (ABIN734003) at 1:200 overnight at 4 °C.

Formalin-fixed and paraffin embedded human artery labeled with Anti-Elastin Polyclonal Antibody, Unconjugated (ABIN734003) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat intervertebral disk labeled with Anti-ELASTIN, Unconjugated (ABIN734003) followed by conjugation to the secondary antibody and DAB staining

Images provided the Independent Validation Program (badge number 28751): Formalin-fixed and paraffin embedded mouse heart labeled with Rabbit Anti-alpha Elastin Polyclonal Antibody (ABIN734003) at 1:1000 4 °C temperature overnight 4 °C followed by conjugation to secondary antibody.

Human A549 cells probed with Rabbit Anti-Elastin Polyclonal Antibody

8 images

4 references

Independent Validation

1 validation

Catalog No. ABIN734003

Datasheet as PDF

Iba1 antibody (AA 51-147)

IBA1 Reactivity: Human, Rat, Mouse WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Immunofluorescence validation image for anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 51-147) antibody (ABIN685477)

Immunofluorescence validation image for anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 51-147) antibody (ABIN685477)

Immunofluorescence validation image for anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 51-147) antibody (ABIN685477)

Immunofluorescence validation image for anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 51-147) antibody (ABIN685477)

Formalin-fixed and paraffin embedded mouse brain labeled with Anti-AIF1/IBa-1 Polyclonal Antibody, Unconjugated (ABIN685477) followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-AIF1 Polyclonal Antibody, Unconjugated used at 1:200 dilution for 40 minutes at 37°C.

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-AIF1 Polyclonal Antibody, unonjugated 1:200, 40 minutes at 37°C

Formalin-fixed and paraffin embedded rat brain labeled with Anti-AIF1/IBa-1 Polyclonal Antibody, Unconjugated (ABIN685477) followed by conjugation to the secondary antibody and DAB staining

BV-microglia stained with AIF1 Polyclonal Antibody at 1:100 dilution.

Independently Validated Antibody, image provided by Science Direct, badge number 029613:Frozen,OCT embedded mouse spleen labeled with Anti-AIF1 Polyclonal Antibody, Unconjugated (ABIN685477) at 1:100 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse spinal cord stained with AIF1 Polyclonal Antibody at 1:100 dilution.

11 images

7 references

Independent Validation

1 validation

Catalog No. ABIN685477

Datasheet as PDF

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