Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Lateral Flow Antibodies

Lateral flow tests are rapid immunochromatographic tests in strip format. They enable the detection of diverse analytes within just a few minutes. In addition to rapid results and ease of use, the advantages of lateral flow tests are low cost and high validity. Since they can be performed without complex infrastructure, they have versatile applications as point-of-care tests (POCT). antibodies-online offers a wide range of high quality antibodies suitable for utilization in lateral flow assays.

Lateral Flow Assay Development

One of the key components to developing successful lateral flow assays is a sensitive and stable conjugate, which acts as a reporter particle. Covalent conjugation of antibodies to gold nanoparticles continues to become more widely used in commercial assays. With this method, less antibody is needed to maximize sensitivity, reducing the overall cost of an assay. The easy and consistent preparation saves time when performing antibody screening experiments. Additionally, the antibody-to-particle ratio can be precisely controlled.

Conjugation of Antibody to Gold Nanoparticles for Lateral Flow Assays

antibodies-online is able to support your lateral flow assay development. Try our high-performance gold conjugates which are ready to use in a lateral-flow assay. The Gold-in-a-Box™ Conjugation Kit enables optimal binding of an antibody/protein to our Naked Gold 20 nm and 40 nm highly concentrated gold sols, while retaining a high degree of specific activity through adjusting the pH of the gold sols to slightly above the isoelectric point of the coating antibody/protein.

Antibodies for Lateral Flow Assay Development

antibodies-online offers a wide range of high quality antibodies suitable for utilization in lateral flow assays. The focus of our portfolio lies on antibodies against compounds of the human body as well as various viruses. In addition, we also offer antibodies against viruses, fungi or bacteria. Discover our complete portfolio down below.
Especially for Lateral Flow Asssay development: Bulk price options available!

Selection of Lateral Flow Publications Citing antibodies-online

  • Drugs of Abuse: Quantitative Point-of-Care Platform for the Assessment of Drugs of Abuse in Urine, Serum, and Whole Blood using ABIN723246 .1
  • Pregnancy: Development of a portable Raman/SERS-LFA reader using ABIN2143148 .2
  • Lyme Disease: Development of a multiplexed point-of-care immunodiagnostic assay for antibody detection in human sera made through the vertical stacking of functional paper layers using ABIN964717 .3
  • Bacterial Pathogens: Development of a Point-of-Care Magnetic Focus Lateral Flow Sensor for the Detection and profiling of pathogenic bacteria using ABIN6391433 .4
  • SARS-CoV-2: Paper-Based Test for Rapid On-Site Screening of SARS-CoV-2 in Clinical Samples using ABIN6990129 .5
  • Influenza: Using ABIN573415 to develop a Dual Recognition Element Lateral Flow Assay Toward Multiplex Strain Specific Influenza Virus Detection. 6

What is a Lateral Flow Test?

Lateral Flow Assays provide a simple and fast way to test a liquid patient sample, such as blood, urine, serum, saliva, sweat or other fluids, for a specific analyte. The first lateral flow test that entered the market was the Clearblue 1-step test in 1988 as a home test for the detection of the pregnancy hormone. In the past years, the technology has been utilized to great success in detection of infectious diseases, especially SARS-CoV-2.

How does a Lateral Flow Immunoassay Work?

Lateral flow tests are another variation of the ELISA in which antigens are immobilized on a strip of cellulose or other suitable matrix. As with other immunological assays, the test method is based on an antigen-antibody reaction.The sample is applied at one end of the strip and any antibodies present diffuse along it until they reach the position where the appropriate antigen is immobilized. Antibodies specific for the antigen bind to and remain at the site of the antigen and are visualized using labeled detection antibody.

Usually, a positive control antigen is placed on the strip so that it is encountered after the test antigen to confirm that the sample has diffused a sufficient distance to interact with the test antigen. More than one test antigen can be dotted on the strip to provide a multiplex test. Lateral flow tests can be used both qualitatively and quantitatively. This is done by interpreting the band intensity of the analyte using an optical reader.

Application Fields for Lateral Flow Assays

Lateral flow tests are widely across many industries sectors for point of care testing, including pharma, environmental testing, animal health, food and feed testing, and plant and crop health. As they can be performed by professionals, trained users or even patients, regardless of the equipment, lateral flow immunoassays are of particular value in medical diagnostic. Lateral flow tests are the most used type of rapid diagnostic test, a medical diagnostic test that is quick and easy to perform and used to confirm the presence or absence of a target analyte, such as pathogens or biomarkers in humans or animals.


References

  1. Li, Uddayasankar, He, Wang, Qin: "Fast, Sensitive, and Quantitative Point-of-Care Platform for the Assessment of Drugs of Abuse in Urine, Serum, and Whole Blood." in: Analytical chemistry, Vol. 89, Issue 16, pp. 8273-8281, (2019) (PubMed).
  2. Ren, Ahmad, Irudayaraj: "16S rRNA Monitoring Point-of-Care Magnetic Focus Lateral Flow Sensor." in: ACS omega, Vol. 6, Issue 16, pp. 11095-11102, (2021) (PubMed).
  3. Ren, Irudayaraj: "Paper-Based Test for Rapid On-Site Screening of SARS-CoV-2 in Clinical Samples." in: Biosensors, Vol. 11, Issue 12, (2022) (PubMed).
  4. Le, Chang, Benton, McCauley, Iqbal, Cass: "Dual Recognition Element Lateral Flow Assay Toward Multiplex Strain Specific Influenza Virus Detection." in: Analytical chemistry, Vol. 89, Issue 12, pp. 6781-6786, (2019) (PubMed).
  5. Tran, Walkenfort, König, Salehi, Schlücker: "Rapid, Quantitative, and Ultrasensitive Point-of-Care Testing: A Portable SERS Reader for Lateral Flow Assays in Clinical Chemistry." in: Angewandte Chemie (International ed. in English), Vol. 58, Issue 2, pp. 442-446, (2020) (PubMed).
  6. Joung, Ballard, Ma, Tseng, Teshome, Burakowski, Garner, Di Carlo, Ozcan: "Paper-based multiplexed vertical flow assay for point-of-care testing." in: Lab on a chip, Vol. 19, Issue 6, pp. 1027-1034, (2019) (PubMed).

Important Targets

All Lateral Flow Antibodies

413 results:

AFP Reactivity: Human LF, ELISA Host: Mouse Monoclonal 6127435 unconjugated
Pubmed 1 reference
Catalog No. ABIN283254
$409.55
Plus shipping costs $45.00
1 mg
local_shipping Shipping to: United States
Delivery in 9 to 11 Business Days
NT-ProBNP Reactivity: Human LF, ELISA Host: Mouse Monoclonal unconjugated
Pubmed 1 reference
Catalog No. ABIN3027762
$497.36
Plus shipping costs $45.00
1 mg
local_shipping Shipping to: United States
Delivery in 9 to 11 Business Days
anti-Nucleoprotein antibody Primary Antibody
Reactivity: Respiratory Syncytial Virus (RSV) DB, LF Host: Mouse Monoclonal 25-2 unconjugated
Pubmed 1 reference
Catalog No. ABIN572890
$637.56
Plus shipping costs $45.00
1 mg
local_shipping Shipping to: United States
Delivery in 2 to 3 Business Days
SARS-CoV-2 N Reactivity: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Alpha, SARS CoV-2 Beta, SARS CoV-2 Delta, SARS CoV-2 Gamma, SARS CoV-2 Omicron ELISA, LF, WB Host: Mouse Monoclonal unconjugated
Catalog No. ABIN6953169
$916.30
Plus shipping costs $45.00
1 mg
local_shipping Shipping to: United States
Delivery in 3 to 4 Business Days
Reactivity: SARS Coronavirus-2 (SARS-CoV-2) ELISA, LF, WB Host: Cell culture Monoclonal unconjugated
Catalog No. ABIN6953296
$1,917.30
Plus shipping costs $45.00
1 mg
local_shipping Shipping to: United States
Delivery in 3 to 4 Business Days
Reactivity: SARS Coronavirus-2 (SARS-CoV-2) ELISA, LF, WB Host: Cell culture Monoclonal unconjugated
Catalog No. ABIN6953297
$1,917.30
Plus shipping costs $45.00
1 mg
local_shipping Shipping to: United States
Delivery in 3 to 4 Business Days
hCG Reactivity: Human ELISA, LF Host: Mouse Monoclonal MH-95 unconjugated
Catalog No. ABIN343851
$140.53
Plus shipping costs $45.00
1 mg
local_shipping Shipping to: United States
Delivery in 6 to 8 Business Days
hCG Reactivity: Human ELISA, LF Host: Mouse Monoclonal MH-109 unconjugated F(ab')2 fragment
Catalog No. ABIN6391425
$156.77
Plus shipping costs $45.00
1 mg
local_shipping Shipping to: United States
Delivery in 6 to 8 Business Days
anti-Biotin antibody Primary Antibody
ELISA, LF Host: Mouse Monoclonal U-10 unconjugated
Catalog No. ABIN343884
$169.09
Plus shipping costs $45.00
1 mg
local_shipping Shipping to: United States
Delivery in 6 to 8 Business Days
HBsAg Reactivity: Hepatitis B Virus (HBV) ELISA, LF Host: Mouse Monoclonal B-15 unconjugated
Catalog No. ABIN343854
$191.38
Plus shipping costs $45.00
1 mg
local_shipping Shipping to: United States
Delivery in 6 to 8 Business Days
  • Type Primary
    • Primary
  • Target
    • Hepatitis B Virus Surface Antigen (HBsAg)
    • Free Prostate Specific Antigen (fPSA)
    • Prostate Specific Antigen (PSA)
    • Tetrahydrocannabinol (THC)
    • Cryptosporidium Parvum
    • Heartworm, Canine
    • Hemoglobin
    • Methylene Dioxymethamphetamine (MDMA)
    • Oxycodone
    • Cardiac Troponin I (TNNI3)
    • Chorionic Gonadotropin, beta Polypeptide (CGB)
    • Creatine Kinase MB (CKM)
    • Creatine Kinase, Muscle (CKM)
    • D-Dimer
    • Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9)
    • Pro-Brain Natriuretic Peptide (NT-ProBNP) (NT-ProBNP)
    • Adenovirus Hexon
    • Dengue Virus NS1 (DENV NS1)
    • Streptococcus Group A
    • Plasmodium falciparum Histidine Rich Protein 2 (Pf HRP2)
    • Rotavirus (RV)
    • alpha-Fetoprotein (AFP)
    • Chorionic Gonadotropin (hCG)
    • Lipocalin 2 (LCN2)
    • Adenovirus (ADV)
    • Clostridium Difficile B Toxin
    • Giardia Lamblia
    • Plasmodium Vivax
    • Troponin I (TNNI)
    • Aflatoxin (AFT)
    • Amphetamine
    • Barbiturate (BAR)
    • C-Reactive Protein (CRP)
    • Chlamydia Trachomatis
    • Clostridium Difficile A Toxin (toxA)
    • Escherichia Coli O157
    • HCG beta
    • Histidine-Rich Protein 2
    • Influenza A Virus
    • Methamphetamine (M-Amp)
    • Morphine
    • Oxazepam
    • Parainfluenza Virus Type 3
    • Plasmodium Falciparum
    • Plasmodium LDH
    • Respiratory Syncytial Virus (RSV)
    • Salmonellas LPS Core
    • Tricyclic Antidepressant
    • Aggrecan (ACAN)
    • Benzoylecgonine (BZE)
    Please use search to find more...
  • Application Lateral Flow (LF)
    • Lateral Flow (LF)
    • Western Blotting (WB)
    • ELISA
    • Immunohistochemistry (IHC)
    • Immunofluorescence (Paraffin-embedded Sections) (IF (p))
    • Flow Cytometry (FACS)
    • Immunofluorescence (Cultured Cells) (IF (cc))
    • Immunofluorescence (IF)
    • Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    • Immunoprecipitation (IP)
    • Immunocytochemistry (ICC)
    • Immunohistochemistry (Frozen Sections) (IHC (fro))
    • Enzyme Immunoassay (EIA)
    • Immunofluorescence (fixed cells) (IF/ICC)
    • Dot Blot (DB)
    • Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    • Immunochromatography (IC)
    • Radioimmunoassay (RIA)
    • Functional Studies (Func)
    • Neutralization (Neut)
    • Immunostaining (ISt)
    • Coating (Coat)
    • Fluorescence Microscopy (FM)
    • Staining Methods (StM)
    • Immunodiffusion (ID)
    • Chromatin Immunoprecipitation (ChIP)
    • Immunoassay (IA)
    • ELISA (Detection)
    • Proximity Ligation Assay (PLA)
    • Confocal Microscopy (CM)
    • Immunoelectrophoresis (IEP)
    • Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))
    • Antibody Array (AA)
    • Blocking Antibody (Inhibition)
    • ELISA (Capture)
    • RNA Interference (RNAi)
    • ChIP DNA-Sequencing (ChIP-seq)
    • Cytotoxicity Test (CyTox)
    • Cellular Assay (CA)
    • Indirect Immunofluorescence Assay (IFA)
    • Blocking Reagent (BR)
    • ELISpot
    • Blocking Peptide (BP)
    • Immunoelectron Microscopy (IEM)
    • FLISA
    • Live Cell Imaging (LCI)
    • Luminex Assay (LMNX)
    • Gel Shift (GS)
    • Protein Assay (PrA)
    • Haemagglutination (H)
    Please use search to find more...
  • Reactivity
    • Human
    • Hepatitis B Virus (HBV)
    • Plasmodium falciparum
    • Dog
    • Cannabis
    • Cryptosporidium parvum
    • Horse
    • Streptococcus
    • Adenovirus
    • Rotavirus
    • Virus
    • Chlamydia trachomatis
    • Plasmodium malariae
    • Plasmodium vivax
    • Respiratory Syncytial Virus (RSV)
    • Vibrio cholerae
    • Dengue Virus
    • E. coli
    • Influenza A Virus
    • Norovirus
    • SARS Coronavirus-2 (SARS-CoV-2)
    • Adeno-Associated Virus (AAV)
    • Aspergillus
    • Chemical
    • Clostridium
    • Clostridium difficile
    • Giardia lamblia
    • Hormone
    • Human Immunodeficiency Virus (HIV)
    • Influenza B Virus
    • Parainfluenza virus type 3
    • Plasmodium
    • Salmonella
    • Canine Parvovirus (CPV)
    • Dengue virus type 2
    • Helicobacter pylori
    • Herpes Simplex Virus (HSV)
    • Human Metapneumovirus (HMPV)
    • Mumps Virus
    • Protozoa
    • Streptococcus pneumoniae
    • Vaccinia Virus (VACV)
    • Aeromonas caviae
    • Agrobacterium sp.
    • Bacillus thuringiensis
    • Bacteria
    • Campylobacter jejuni
    • Candida albicans
    • Canine Distemper Virus (CDV)
    • Dengue virus type 3
    Please use search to find more...
  • Cited in publications
  • Images available
  • Preservative free only
  • Host
    • Mouse
    • Rabbit
    • Goat
    • Cell culture
    • Chicken
    • Sheep
    • Rat
  • Clonality
    • Monoclonal
    • Polyclonal
    • Chimeric
  • Clone
    • 9L173
    • 1-B-9E13
    • 1-B-9E14
    • 1-B-9E16
    • 1-BB-948
    • 11C211
    • 12H18
    • 14D53
    • 1E1K11
    • 9L314
    • 14D118
    • 14D119
    • 3B6
    • B1242M
    • B492M
    • B525M
    • B879M
    • J-2
    • MH-95
    • 1-B-903
    • 1-B-904
    • 1-B-906
    • 10D46
    • 1131
    • 115-11487
    • 12D6
    • 13C1
    • 15C18
    • 18D4
    • 1E11
    • 1G8
    • 1H12
    • 2-00E-004
    • 25-2
    • 28A4
    • 2B3-G6
    • 31D
    • 32CD
    • 3C9
    • 3C92
    • 4C10A7
    • 4D1-P15
    • 5A9D12
    • 6127435
    • 67A
    • 6F9
    • 77F12
    • 8A6
    • 8B10
    • 8C7
    Please use search to find more...
  • Binding Specificity
    • total
    • Subtype ad/ay
    • Free Chain
    • Trimer
    • AA 10-25
    • AA 13-22
    • AA 13-27
    • AA 169-184
    • AA 185-196
    • AA 23-29
    • AA 24-40
    • AA 27-31
    • AA 27-43
    • AA 38-44
    • AA 39-76
    • AA 41-49
    • AA 61-76
    • AA 80-95
    • Domain 4
    • N-Term
    • Surface
  • Conjugate
    • Un-conjugated
    • Biotin
    • FITC
    • HRP
    • APC
    • Alkaline Phosphatase (AP)
    • PE
  • Isotype
    • IgG1
    • IgG2a
    • IgG1 kappa
    • IgG
    • IgG2b
    • IgG3
    • IgG2a kappa
    • IgG2b kappa
    • IgG IgA IgM Mix
    • IgG3 kappa
    • IgG kappa
    • IgG1 lambda
    • IgG2
    • IgG2a lambda
    • IgG2b lambda
    • IgM
  • Protein Type
    • Recombinant Antibody
  • Format
    • Liquid
    • Lyophilized
    • Frozen
  • Fragment
    • F(ab')2 fragment
  • Supplier
    • antibodies-online
    • USBio
    • Abbexa
    • GenScript
    • Acris
    • RayBiotech
You are here:
help Support