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Resources

CUT&RUN Combo: Beads, Controls, Primary, MNase in One go!

CUT&RUN stands for "Cleavage Under Targets and Release Using Nuclease". CUT&RUN introduces some major modifications to eliminate shortcomings inherent to ChIP-seq....

GFP Catcher - High-Affinity Single-Domain Antibodies

antibodies-online offers catchers for / ideal tool. Our current product lines are based on recombinant single-domain antibodies (sdAbs) derived from llamas or alpacas. This type of...

antibodies-online CUT&RUN and CUT&Tag Handbook

antibodies-online CUT&RUN and CUT&Tag Handbook

antibodies-online SARS-CoV-2 Handbook

antibodies-online SARS-CoV-2 Handbook

antibodies-online™ High Quality ELISA Kits

antibodies-online™ ELISA kits are hand-picked kits, whose individual components have been tested extensively.

Share your validation data and get your money back

Be part of our Independent Validation Initiative. Validate one of our products and get your money back.

Conjugates Labels Tags

Conjugates Labels Tags

Independent Validation Initiative

Most commercially available antibodies lack critical validation data. This is why we started the (IVI) to independently validate commercialantibodies and ELISA kits. The Independent Validation Initiative is centered...

Nanodiscs

Nanodiscs are soluble synthetic phospholipid bilayers that are encircled by a belt of helical membrane scaffold proteins. They enable reconstitution and detailed study of membrane proteins.

An Introduction to Fluorescence (Part 2)

Fluorescence is among the most extensively studied of all natural phenomena. For most fluorescent probes there are a set of precise measurements that can help a researcher accurately...

The Epigenome Project

The frugality of the human genome contrasts with the profound complexity and diversity of the human species, and poses a number of interesting questions. Namely, how do you build such a stunningly complex system with...

An Introduction to Fluorescence (Part 1)

Fluorescence is a property possessed by certain materials, known as fluorophores, by which these materials absorb short-wavelength incident radiation and emit longer-wavelength radiation.

Five tips for choosing the perfect primary antibody

Here are a few tips to help you minimize the amount of time you spend searching for the right antibody so that you can get your experiment up and running as quickly as possible

Antibody Arrays

Your ELISA kit is missing something! If you're still using ELISA for biomarker analysis, you're missing out on vital insight. You're also leaving time, money, and sample on the table. Learn about...

Enzyme Inhibitors

Modern drug development is about specificity. Enzyme inhibitors offer a powerful method for blocking specific activity at the metabolic level.

Monoclonal antibodies

In 1975 G. Köhler and C. Milstein published in Nature the introduction of a hybridoma technique to produce monoclonal antibodies. This technique has become a pillar in modern biological science

Antibodies

Antibodies are an important element of the so-called humoral (antibody-mediated) immunity. They belong to the gamma globulin fraction and are also called immunoglobulins (Ig).

Polyclonal antibodies

Polyklonal antibodies are produced by immunizing animals. The animals used most commonly for the production of antibodies are rats, rabbits, goats, sheep and horses.

anti-Immunoglobulin G (IgG) secondary antibodies

Each IgG is composed of four peptide chains - two class γ heavy chains of about 50 kDa and two light chains of about 25 kDa - with two antigen binding sites. Hence, IgG antibodies are...

anti-Immunoglobulin A (IgA) secondary antibodies

Immunoglobulin A plays a role in mucosal immunity and is produced in mucosal linings. Hence IgA can be found in mucous secretions, including tears, saliva, colostrum, secretions from the...

anti-Immunoglobulin E (IgE) secondary antibodies

IgE is only found in mammals. IgE is the least abundant isotype of all antibodies, but it is capable of triggering the most powerful immune reactions. IgE plays an important role in...

anti-Immunoglobulin D (IgD) Secondary Antibodies

Immunoglobulin D is a monomeric antibody isotype that is expressed in the plasma membranes of immature B-lymphocytes. IgD is also produced in a secreted form that is found in small...

anti-Immunoglobulin M (IgM) secondary antibodies

IgM is constructed of five or six units (i.e. mostly as pentamers but also hexamers occur) which are each comprised of two heavy-chains (mu-chains) and two light chains, bound together by...

anti-Immunoglobulin Y (IgY) secondary antibodies

Immunoglobulin Y (IgY) is found in birds, and it is being isolated for biotechnological applications from egg-yolk of chicken eggs. Studies have suggested that Immunoglobulin Y is...

CUT&RUN - Improved Method to Study Protein-DNA Interactions

CUT&RUN-sequencing, also known as Cleavage Under Targets and Release Using Nuclease, is a method used to analyze protein interactions with DNA....

Chromatin immunoprecipitation (ChIP)

Immunoprecipitation of protein-DNA complexes (ChIP) enables the study of epigenetic relations. The technique identifies binding sites of DNA-associated proteins and allows a closer study of transcription factors.

Protein Expression Systems

Production of functional proteins is a crucial step for scientist in order to investigate the function and structure requires of proteins of interest.

Biotinylated Proteins: Better insight through better binding

The bond between Biotin and Streptavidin is one of the strongest non-covalent bonds ever identified. It is frequently exploited in molecular biology to...

Magnetic Beads

Magnetic beads are an ideal tool for immobilizing molecules like proteins (enzymes, antibodies, peptides etc.) or nucleic acids on a solid phase; they can be further used in various applications.

Peptide Synthesis - Methods and Reagents

In organic chemistry, peptide synthesis is the production of peptides, in which multiple amino acids are linked via peptide bonds.

What is a nanobody?

A nanobody, also known as a single-domain antibody (sdAb), is an antibody fragment consisting of a single monomeric variable antibody domain.

Immunoprecipitation, CoIP, and ChIP-seq

At its core, immunoprecipitation is used to isolate a single protein. Various evolutions have turned this simple method into one of the mainstays in life science research.

Smartox Biotechnology: Synthetic Polypeptide Toxins for Research Applications

Toxins have always captured the interest of curious biomedical and life-science researchers. Early toxicological research predominantly focused on...

Proximity Ligation Assay

Traditional methods for identifying protein-protein interactions include a host of complex experiments like two-hybrid assays and co-immunoprecipitations. While these experiments do provide the opportunity to assay...

Gel electrophoresis

Electrophoresis is a common molecular biology technique that is used to separate biological materials by virtue of their size and/or charge.

An Introduction to ELISA (Part 2)

ELISA is an exceptionally powerful and versatile technique, and there are number of different ways to approach it. This article covers the various ELISA formats that are most commonly commercially available, and...

An Introduction to ELISA (Part 1)

While there are many different kinds of ELISAs, the basic principle behind the assay is strikingly simple: antibodies conjugated to an enzyme (most commonly a peroxidase) are bound to an analyte that has been...

Radioimmunoassay (RIA)

Radioimmunoassay (RIA) is an in vitro assay that measures the presence of an antigen with very high sensitivity. Basically any biological substance for which a specific antibody exists can be measured, even in minute...

Immunohistochemistry (IHC)

Immunohistochemistry (IHC) is a method that allows detection of proteins or other antigens in tissue sections. For this purpose the sections are exposed to labelled antibodies that are directed against epitopes in...

Loading Controls

Western blotting is a commonly used method to determine specific protein levels in a sample (e.g. tissue homogenate, extract). Loading control antibodies...

Western Blotting (Immunoblot): Gel Electrophoresis for Proteins

Western Blotting (also called immunoblotting) is a technique used for analysis of individual proteins in a protein mixture (e.g. a cell lysate). In Western...

Whole cell lysates

antibodies-online.com offers whole cell lysates from human, mouse, rat or chicken. These tissue lysates can be used in applications such as SDS-PAGE and Western blotting.

Enzyme-linked immunosorbent assay (ELISA)

Enzyme-linked Immunosorbent assay (ELISA) can provide a useful measurement of antigen or antibody concentration.

What is Flow Cytometry (FACS Analysis)?

Originally developed in the late 1960s, flow cytometry is a popular analytical cell-biology technique that utilizes light to count and profile cells in a heterogenous fluid mixture. Flow...

Antibody purification using antibody-binding proteins

Protein L are native and recombinant proteins of microbial origin that bind to mammalian immunoglobulins (Ig). Protein A and protein G bind with high affinity and specificity to the Fc...

SARS-CoV-2 Detection using RT-PCR Kits and qPCR Kits

Fast and accurate testing solutions for COVID-19 - We offer a hand-selected variety of qPCR and RT-PCR kits for the detection of SARS-CoV-2.

SARS-CoV-2 Life Cycle: Stages and Inhibition Targets

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) belongs to the enveloped positive-sense RNA viruses. This virus is characterized by club-like spikes on the surface, and a...

SARS-CoV-2 / 2019-nCoV: Vaccines, Drug Discovery & Diagnostics to prevent and control Infections

Developing vaccines against SARS-CoV-2, working on new therapeutics or repurposing existings drugs for SARS-CoV-2 is key when...

Coronavirus (2019-nCoV) Research Antibodies, Proteins and Kits

antibodies-online has served hundreds of research institutions around the world with Coronavirus (2019-nCoV) Research Antibodies, Proteins and Kits since the...

Model Organisms

When researchers look for an organism to use in their studies, they look for several traits. Among these are size, generation time, accessibility, manipulation, genetics, conservation of mechanisms, and potential...

Antibodies and ELISA Kits for Tags

Epitope Tag Antibodies, ELISA Kits, Epitope Tags

Products for Plant-specific Targets

Modern molecular biology has opened up a whole new range of techniques and possibilities to scientists working in the different fields of the classical plant sciences like plant physiology, breeding or pathology.

MeDIP, hMeDIP, bisulfite sequencing, and TAB-seq

Modification at the 5-carbon of cytosine in DNA is an epigenetic mark that influences gene expression, cell differentiation and embryonic development. While 5-mC is the most common and...

Protein Exosome Marker

Exosomes are small (50-120nm) endosome derived extracellular microvesicles (EMV). They can contain a vast array of different proteins depending on their host cell which, and their components are further modulated by...

CD Marker Panel

CD is an abbreviation “for cluster of differentiation”. CD molecules are cell surface markers which are very useful for the identification and characterization of leukocytes and the different subpopulations of...

Protein Tumor Markers

Biomarkers that are produced at higher levels in cancerous conditions compared to benign conditions are collectively termed tumor markers. Most but not all of these substances are proteins that can be measured.

Antibody and immunoglobulin alternatives (Part 2)

The common characteristic for antibody mimetics is the intention to provide alternatives that perform similar or better than antibodies. Their origins are very diverse and accordingly...

Antibody and immunoglobulin alternatives (Part 1)

There is mounting pressure to develop and optimize alternatives to traditional immunoglobulins. Both products derived from immunoglobulins and non Ig-based alternatives are rising in...

Antimicrobial peptides and the innate immune system

Anyone who has taken a collegiate level introductory course in microbiology, medicine, or immunology is aware of the fact that modern multicellular organisms are constantly under assault...

Cytokeratins in the detection of tumors

Cytokeratins (CKs, or following more recent nomenclature also simply called keratins) are intermediate filament forming proteins that provide mechanical support and fulfill a variety of...

Major Histocompatability Complex (MHC)

The major histocompatability complex (MHC) includes a variety of genes in vertebrates, which are responsible for the histocompatibility of...

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