Anti-Mouse Secondary Antibodies

Choose from a handpicked selection of high quality mouse secondary antibodies with enzymatic conjugates like AP, Biotin or HRP or fluorescent conjugates including FITC, APC and DyLight and Atto dye families. Browse our offer for a variety of different host, isotypes or binding specifities.

Anti-Mouse Secondary Antibodies with Enzymatic Conjugates

Secondary antibodies linked with enzymes such as HRP and alkaline phosphatase AP are commonly utilized in applications such as ELISA or Western blotting. Both can be detected using colorimetric or chemiluminescent readout.

Anti-Mouse Secondary Antibodies with Fluorescent Conjugates

Secondary antibodies conjugated to fluorescent proteins, eg FITC, TRITC, APC or PE or commercial dye families excel in flow cytometry or immunofluorescence experiments.

Anti-Mouse Secondary Antibodies conjugated with DyLight Family

Development of DyLight Fluors improved photostability, brightness, and decreadsed pH-sensitivity. The excitation and emission spectra of the DyLight Fluor series cover much of the visible spectrum and extend into the infrared region. DyLight flurorescent conjugates therefore are an excellent alternative to IRDye800CW or IRDye600CW, allowing detection using most fluorescence microscopes, as well as infrared imaging systems.

Anti-Mouse Secondary Antibodies conjugated with Atto Dyes

Atto dyes are photostable and highly resistant to ozone degradation, making them ideal for microarray applications. Signal decay times of 0.6–4.1 nanoseconds allow timegate studies to reduce autofluorescence background and scattering.

Goat is the predominant host species to produce polyclonal anti-mouse secondary antibodies. Your experiments require a different host species? antibodies-online offers anti-mouse secondary antibodies raised in variety of species like rabbit, rat or donkey.

Polyclonal primary antibodies raised in mouse are generally IgG isotypes. Therefore you will need a secondary antibody that is specific to mouse IgG (anti-mouse IgG) which reacts with the heavy and light chains of IgG. You are working with primary mouse monoclonals and need a specific isotype for your secondary? Browse our selection below.

Anti-Mouse Secondary Antibodies Fragments

Secondary antibody fragments offer special options in detection of primary antibodies. Alpaca single domain antibodies for example contain only heavy chains and recognize Fab and Fc fragments of mouse IgG. F(ab')2 fragments on the other hand lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. The small size enables better tissue penetration, antigen access and decreases the distance between the epitope and label leading to better signal generation in IHC and higher resolution images.

Anti-Mouse Secondary Antibodies for Specific Binding Sites

Anti-IgG, Fc fragment specific antibodies may be used to detect native IgG primary antibodies without binding to the 25 kDa band of reduced and denatured IgG light chains on Western blots.

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