Mouse Naive/Memory T cell ID Panel

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Mouse (Murine)
Flow Cytometry (FACS)
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Characteristics In mice, CD4+ T cells can be divided into naive and memory cells based on expression of adhesion molecules CD44 and CD62L. Naive T cells exhibit high levels of CD62L and low expression of CD44, whereas memory T cells are identified by high CD44 and low CD62L expression. This panel includes antibodies against CD3, CD4, CD62L and CD44, which can be used together to identify naive and memory T cell subsets in mice. Alterations in these subsets are evident in older mice following antigenic stimulation.
Components Mouse Naive/Memory T cell ID Panel, Rat IgG2b/Rat IgG2a/Rat IgG2a/Rat IgG2b
Application Notes This reagent contains one vial each of APC/Cy7 conjugated anti-CD3 (clone 17A2), PerCP/Cy5.5 conjugated anti-CD4 (clone RM4-5), APC conjugated anti-CD62L (clone MEL-14), and PE conjugated anti-CD44 (clone IM7) at optimal concentration for four-color flow cytometric analysis.
Restrictions For Research Use only
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.
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Flow Cytometry (FACS) image for Mouse Naive/Memory T cell ID Panel (ABIN2669200) Mouse Naive/Memory T cell ID Panel
Flow Cytometry (FACS) image for Mouse Naive/Memory T cell ID Panel (ABIN2669200) Mouse Naive/Memory T cell ID Panel (Image 2)
Background publications Tedder, Steeber, Pizcueta: "L-selectin-deficient mice have impaired leukocyte recruitment into inflammatory sites." in: The Journal of experimental medicine, Vol. 181, Issue 6, pp. 2259-64, 1995 (PubMed).

Weiss, Littman: "Signal transduction by lymphocyte antigen receptors." in: Cell, Vol. 76, Issue 2, pp. 263-74, 1994 (PubMed).

Haynes, Liao, Patton: "The transmembrane hyaluronate receptor (CD44): multiple functions, multiple forms." in: Cancer cells (Cold Spring Harbor, N.Y. : 1989), Vol. 3, Issue 9, pp. 347-50, 1992 (PubMed).

Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, 1992 (PubMed).

Davis: "T cell receptor gene diversity and selection." in: Annual review of biochemistry, Vol. 59, pp. 475-96, 1990 (PubMed).

Kishimoto, Jutila, Butcher: "Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 6, pp. 2244-8, 1990 (PubMed).

Bierer, Sleckman, Ratnofsky, Burakoff: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, 1989 (PubMed).

Goldstein, Zhou, Picker, Minty, Bargatze, Ding, Butcher: "A human lymphocyte homing receptor, the hermes antigen, is related to cartilage proteoglycan core and link proteins." in: Cell, Vol. 56, Issue 6, pp. 1063-72, 1989 (PubMed).