Goat anti-Mouse IgG (Heavy & Light Chain) Antibody (Texas Red (TR)) - Preadsorbed
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- Target See all IgG products
- IgG
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Binding Specificity
- Heavy & Light Chain
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Reactivity
- Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- Texas Red (TR)
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Application
- Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)
- Supplier Product No.
- 610-109-121
- Supplier
- Rockland
- Purpose
- Mouse IgG (H&L) Antibody Texas Red™ Conjugated Pre-Adsorbed
- Cross-Reactivity (Details)
- Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
- Characteristics
- Anti-Mouse IgG + IgM Texas Red Antibody generated in Goat detects reactivity to Mouse IgG and IgM.
- Purification
- Mouse IgG (H&L) Antibody Texas Red™ Conjugated Pre-Adsorbed was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- Immunogen
- Optional[Immunogen]: Mouse IgG whole molecule
- Isotype
- IgG
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- Application Notes
- Application Note: Mouse IgG (H&L) Antibody Texas Red™ Conjugated Pre-Adsorbed antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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Expression of Muscle-Specific Ribosomal Protein L3-Like Impairs Myotube Growth." in: Journal of cellular physiology, Vol. 231, Issue 9, pp. 1894-902, (2017) (PubMed).
: "Inducible depletion of satellite cells in adult, sedentary mice impairs muscle regenerative capacity without affecting sarcopenia." in: Nature medicine, Vol. 21, Issue 1, pp. 76-80, (2015) (PubMed).
: "Automated fiber-type-specific cross-sectional area assessment and myonuclei counting in skeletal muscle." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 115, Issue 11, pp. 1714-24, (2014) (PubMed).
: "Regulation of the muscle fiber microenvironment by activated satellite cells during hypertrophy." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 28, Issue 4, pp. 1654-65, (2014) (PubMed).
: "Automated image analysis of skeletal muscle fiber cross-sectional area." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 114, Issue 1, pp. 148-55, (2013) (PubMed).
: "Effective fiber hypertrophy in satellite cell-depleted skeletal muscle." in: Development (Cambridge, England), Vol. 138, Issue 17, pp. 3657-66, (2011) (PubMed).
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Expression of Muscle-Specific Ribosomal Protein L3-Like Impairs Myotube Growth." in: Journal of cellular physiology, Vol. 231, Issue 9, pp. 1894-902, (2017) (PubMed).
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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