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CD9 antibody (CD9) Biotin

Details for Product anti-CD9 Antibody No. ABIN1043025, Supplier: Login to see New
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Synonyms Tspan29, BTCC-1, DRAP-27, MIC3, MRP-1, TSPAN-29, TSPAN29
Mouse (Murine)
(350), (87), (28), (27), (22), (21), (21), (20), (13), (8), (7), (7), (7), (7), (6), (6), (1), (1)
(283), (78), (60)
Clonality (Clone)
Monoclonal ()
This CD9 antibody is conjugated to Biotin
(60), (60), (33), (16), (5), (5), (3), (2), (1), (1), (1)
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunocytochemistry (ICC)
(315), (191), (96), (75), (63), (47), (45), (38), (27), (24), (6), (4), (3), (2), (2), (2), (2), (2), (1), (1)
Pubmed 5 references available
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Quantity 0.1 mg
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Availability Will be delivered in 4 to 5 Business Days
Immunogen Permeabilized murine bone marrow-derived mast cells (BMMC).
Clone EM-04
Isotype IgG1
Specificity The rat monoclonal antibody EM-04 recognizes CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.
Characteristics The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Alternative Name CD9 (CD9 Antibody Abstract)
Background CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.
Gene ID 12527
Research Area Embryogenesis, Embryonic stem cells, Reproduction, CD Antigens, Surface Receptors of Immune Cells
Application Notes Reagent is designed for flow cytometry analysis.

Working concentrations should be determined by the investigator.

CD and Related Antigens & MHC (Rodent)

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
Western Blotting (WB) image for anti-CD9 antibody (CD9)  Biotin (ABIN1043025) Detection of CD9 inbone marrow-derived mast cell (BMMC) lysates by rat monoclonal EM-...
Background publications Kotha, Zhang, Longhurst et al.: "Functional relevance of tetraspanin CD9 in vascular smooth muscle cell injury phenotypes: a novel target for the prevention of neointimal hyperplasia." in: Atherosclerosis, Vol. 203, Issue 2, pp. 377-86, 2009 (PubMed).

Unternaehrer, Chow, Pypaert et al.: "The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 1, pp. 234-9, 2007 (PubMed).

Liu, Cao, Yang et al.: "Tetraspanin CD9 regulates invasion during mouse embryo implantation." in: Journal of molecular endocrinology, Vol. 36, Issue 1, pp. 121-30, 2006 (PubMed).

Le Naour, Rubinstein, Jasmin et al.: "Severely reduced female fertility in CD9-deficient mice." in: Science (New York, N.Y.), Vol. 287, Issue 5451, pp. 319-21, 2000 (PubMed).

Schmidt, Künemund, Wintergerst et al.: "CD9 of mouse brain is implicated in neurite outgrowth and cell migration in vitro and is associated with the alpha 6/beta 1 integrin and the neural adhesion molecule L1." in: Journal of neuroscience research, Vol. 43, Issue 1, pp. 12-31, 1996 (PubMed).

Catalog No. ABIN1043025
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