NF-Y (N-Term) antibody

Details for Product No. ABIN104769
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Western Blotting (WB), ELISA
(3), (2), (2), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN104769
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Immunogen Anti-NF-Y (A) antibody was produced by repeated immunizations with a synthetic NF-Y (A subunit) peptide corresponding to a region near the of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Background Anti-NF-Y antibody detects NF-Y (a subunit) protein. NF-Y is a highly conserved transcription factor that stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. NF-subunit A associates with a tight dimer composed of the NF-YB and NF-YC subunits, resulting in a trimer that binds to DNA with high specificity and affinity. Anti-NF-YA Antibody is ideal for investigators involved in Cell Signaling, Immunology, Cancer, and Signal Transduction research.
Synonyms: Nf-Y-A subunit, CAAT box DNA-binding protein subunit A, Nuclear transcription factor Y subunit A
Gene ID 4800
UniProt P23511
Research Area Transcription Factors
Application Notes Anti-NF-Y antibody is suitable for western blotting and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-NF-Y (N-Term) antibody Western Blot of Rabbit Anti-NF-Y(A subunit) Antibody. Lane 1: CHO-7 cells in the absence (I) of cholesterol. Lane 2: CHO-7 cells in the presence (S) of cholesterol. Load: Equivalent aliquots of chromatin from each sample containing ~50 µg of total protein per lane. Primary antibody: anti-NF-YA antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP conjugated Goat-anti-Rabbit IgG [H&L] secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: doublet of ~ 42 kDa for NF-Y A.
General Osborne, Zhang, Yang et al.: "Histone deacetylase activity represses gamma interferon-inducible HLA-DR gene expression following the establishment of a DNase I-hypersensitive chromatin conformation." in: Molecular and cellular biology, Vol. 21, Issue 19, pp. 6495-506, 2001 (PubMed).

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