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CADM2 antibody (Internal Region)

CADM2 Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Bat, Horse, Monkey, Rabbit, Hamster, Guinea Pig, Xenopus laevis IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1048369
  • Target See all CADM2 Antibodies
    CADM2 (Cell Adhesion Molecule 2 (CADM2))
    Binding Specificity
    • 16
    • 8
    • 8
    • 6
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 37
    • 35
    • 18
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Cow, Pig, Dog, Bat, Horse, Monkey, Rabbit, Hamster, Guinea Pig, Xenopus laevis
    Host
    • 54
    Rabbit
    Clonality
    • 54
    Polyclonal
    Conjugate
    • 19
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CADM2 antibody is un-conjugated
    Application
    • 43
    • 21
    • 17
    • 13
    • 13
    • 7
    • 7
    • 5
    • 3
    • 3
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human CADM2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Predicted Reactivity
    Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Guinea pig, Zebra finch, Platypus, Xenopus (100%) Turkey, Chicken (94%) Zebrafish (82%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic 17 amino acid peptide from internal region of human CADM2. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Guinea pig, Zebra finch, Platypus, Xenopus (100%), Turkey, Chicken (94%), Zebrafish (82%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product CADM2 Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (2.5 μg/mL)

    Usage: Antigen retrieval with HIER and sodium citrate at  pH 6.0 is required for Immunohistochemistry using formalin-fixed paraffin-embedded human tissues.
    Comment

    Target Species of Antibody: Human

    Assay Procedure

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    CADM2 (Cell Adhesion Molecule 2 (CADM2))
    Alternative Name
    CADM2 (CADM2 Products)
    Synonyms
    2900078E11Rik antibody, 9330131D06 antibody, A830029E02Rik antibody, Igdf4d antibody, Igsf4d antibody, NECL3 antibody, SynCAM 2 antibody, SynCAM2 antibody, Necl-3 antibody, IGSF4D antibody, synCAM2 antibody, igsf4d antibody, zgc:66339 antibody, cell adhesion molecule 2 antibody, cell adhesion molecule 2a antibody, CADM2 antibody, Cadm2 antibody, cadm2a antibody
    Background
    Name/Gene ID: CADM2

    Synonyms: CADM2, Cell adhesion molecule 2, IGSF4D, Necl-3, Nectin-like 3, SynCAM2, Nectin-like protein 3, SynCAM 2, NECL3
    Gene ID
    253559
    Pathways
    Cell-Cell Junction Organization
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