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Inhibitor of Growth Family, Member 4 (ING4) (AA 164-175), (Isoform 1) antibody

Details for Product No. ABIN105223
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Antigen
Synonyms wu:fb11d09, wu:fb50e02, zgc:110619, ING4, my036, p29ING4, D6Wsu147e, D6Xrf92
Epitope
AA 164-175, Isoform 1
(5), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(36), (22), (18), (4), (3), (2), (1)
Host
Goat
(28), (6), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), ELISA
(26), (15), (10), (9), (4), (3), (2), (2), (1)
Pubmed 2 references available
Quantity 100 µg
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Catalog No. ABIN105223
361.90 $
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Immunogen This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 164-175 of Human p29 ING4 protein (Inhibitor of growth family, member 4). This sequence only shows homology to for ING4.
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Alternative Name p29 ING4
Background P29 ING4 is a tumor suppressor protein similar to ING1 that  may inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. p29 ING4 has been shown to suppress brain tumor angio-genesis through transcriptional repression of RelA/ NFKB3 target genes when complexed with RelA.  p29 ING4 may also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. p29 ING4 shows a nuclear localization and interacts with EP300, TP53, RelA, inhibits cell growth, and induces apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. Multiple alternatively spliced transcript variants have been observed.  The accession number listed below is for variant (1) that encodes the longest isoform.
Synonyms: NG-4 antibody, ING1 like protein antibody, Inhibitor of growth family member 4 antibody, Inhibitor of growth family member 4 long isoform antibody, Inhibitor of growth protein 4 antibody, MGC12557 antibody
Gene ID 51147
NCBI Accession AAH13038.2
UniProt Q9UNL4
Application Notes This affinity-purified antibody has been tested for use in ELISA against the immunizing peptide.  Specific conditions for western blotting reactivity should be optimized by the end user.  Expect a band at approximately 29 kDa in size corresponding to ING4 isoform 1 by western blotting in the appropriate cell lysate or extract.
Restrictions For Research Use only
Format Liquid
Concentration 1.28 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Inhibitor of Growth Family, Member 4 (ING4) (AA 164-175), (Isoform 1) antibody Anti-p29 ING4 polyclonal antibody detects ING4 protein by western blot in over expressed cell lysates.  This antibody was used at 1.0 µg/ml to detect ING4 expression in control (-) and transformed U2OS and HeLa cell lysates.  A predominant band corresponding to p29 ING4 is only seen in lysates from transformed cells.  Personnel Communication, Motoko Unoki, NCI, NIH.
anti-Inhibitor of Growth Family, Member 4 (ING4) (AA 164-175), (Isoform 1) antibody (2) Anti-p29 ING4 polyclonal antibody detects ING4 protein by western blot in over expressed cell lysates. This antibody was used at 1.0 ug/ml to detect ING4 expression in control (-) and transformed U2OS and Hcell lysates. A predominant band corresponding to p29 ING4 is only seen in lysates from transformed cells. Personnel Communication, Motoko Unoki, NCI, NIH.
General Unoki, Shen, Zheng et al.: "Novel splice variants of ING4 and their possible roles in the regulation of cell growth and motility." in: The Journal of biological chemistry, Vol. 281, Issue 45, pp. 34677-86, 2006 (PubMed).

Colla, Tagliaferri, Morandi et al.: "The new tumor-suppressor gene inhibitor of growth family member 4 (ING4) regulates the production of proangiogenic molecules by myeloma cells and suppresses hypoxia-inducible factor-1 alpha ..." in: Blood, Vol. 110, Issue 13, pp. 4464-75, 2007 (PubMed).

Validation Images
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