Keratin 8/18/19 (KRT8/18/19) antibody

Antigen: Keratin 8/18/19 (KRT8/18/19)

Clonality: Monoclonal (2A4)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN108431

Additional Information

Alternative name: Cytokeratin 8,18,19

Immunogen: Monoclonal antibody to cytokeratin is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Cytokeratins isolated from cultured pig kidney epithelial cells were used as immunogen

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: 2A4

Description: Monoclonal Antibody to Human Cytoskeletal Polypeptides

Specificity: The antibody recognizes cytokeratin nos. 8, 18 and 19 typical for simple epithelial cells

Application Details

Application Notes: No reactivity with paraffin sections. Working dilution in western blotting is at least 1:1000

Storage: This product should be stored at +2 C to +8 C

Research Area: Cytoskeleton

Restrictions: For Research Use only

Images

Renal cell carcinoma (MAb 2A4, cytokeratin 8, 18, 19)

Publications

Publications: Takumida M, Ylikoski J, Pirvola U et al.: "Intermediate filaments in the endolymphatic sac of the guinea pig." in: ORL J Otorhinolaryngol Relat Spec, Vol. 53, Issue 1, pp. 10-4, 1991 (PubMed).

Heikinheimo K, Persson S, Kindblom LG et al.: "Expression of different cytokeratin subclasses in human chordoma." in: J Pathol, Vol. 164, Issue 2, pp. 145-50, 1991 (PubMed).

Virtanen I, Miettinen M, Lehto VP et al.: "Diagnostic application of monoclonal antibodies to intermediate filaments." in: Ann N Y Acad Sci, Vol. 455, pp. 635-48, 1985 (PubMed).

Miettinen, Lehto, Virtanen: "Antibodies to intermediate filament proteins in the diagnosis and classification of human tumors." in: Ultrastructural pathology, Vol. 7, Issue 2-3, pp. 83-107, 1985 (PubMed).

Holthofer H, Miettinen A, Paasivuo R et al.: "Cellular origin and differentiation of renal carcinomas. A fluorescence microscopic study with kidney-specific antibodies, antiintermediate filament antibodies, and lectins." in: Lab Invest, Vol. 49, Issue 3, pp. 317-26, 1983 (PubMed).

Akasofu M, Kawahara E, Kurumaya H et al.: "Immunohistochemical detection of breast specific antigens and cytokeratins in metastatic breast carcinoma in the liver." in: Acta Pathol Jpn, Vol. 43, Issue 12, pp. 736-44, 1993 (PubMed).

Ansai S, Katagata Y, Yoshikawa K et al.: "An immunohistochemical study of sebaceous carcinoma with anti-keratin monoclonal antibodies: comparison with other skin cancers." in: J Dermatol, Vol. 21, Issue 8, pp. 553-9, 1994 (PubMed).

Oda Y, Hashimoto H, Tsuneyoshi M et al.: "Benign glandular peripheral nerve sheath tumor. A case report." in: Pathol Res Pract, Vol. 190, Issue 5, pp. 466-73, 1994 (PubMed).

Savolainen VT, Lalu K, Penttila A et al.: "Cytokeratin inclusions in alcoholic liver disease and their relation to the amount of alcohol intake." in: Liver, Vol. 14, Issue 6, pp. 281-7, 1994 (PubMed).

Rahman SA, Tsuyama S: "Immunohistochemical study of cell proliferation and differentiation in epidermis of mice after administration of cholera toxin." in: Arch Dermatol Res, Vol. 285, Issue 1-2, pp. 27-31, 1993 (PubMed).

Nogita T, Ohta A, Hidano A et al.: "Basal cell carcinoma with eccrine differentiation." in: J Dermatol, Vol. 22, Issue 2, pp. 111-5, 1995 (PubMed).

Korhonen M, Sariola H, Gould VE et al.: "Integrins and laminins in human renal carcinoma cells and tumors grown in nude mice." in: Cancer Res, Vol. 54, Issue 16, pp. 4532-8, 1994 (PubMed).

Fukunaga M, Endo Y, Ishikawa E et al.: "Mixed tumour of the vagina." in: Histopathology, Vol. 28, Issue 5, pp. 457-61, 1996 (PubMed).