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Heterogeneous Nuclear Ribonucleoprotein A1 (HNRNPA1) antibody

Details for Product No. ABIN108610
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Antigen
Synonyms hnrpa1, TPT1P, HNRPA1, HNRNPA1, hnRNP A1, HNRPA1L3, hnRNP-A1, D15Ertd119e, Hdp, Hnrpa1, hnrnp-A1, ROA1
Reactivity
Mouse (Murine), Human
(57), (23), (21), (3), (3), (2), (1), (1), (1)
Host
Mouse
(44), (18)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)
(62), (40), (30), (25), (16), (15), (7), (1), (1)
Pubmed 2 references available
Quantity 50 µL
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Catalog No. ABIN108610
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Immunogen Full length hnRNP-A1 native protein (partially purified) from HeLa cells (human)
Clone 9H10
Isotype IgG2b
Characteristics Myeloma fusion partners:
Balb/c Sp2/0 cell line
Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa)
Purification Protein A
Alternative Name hnRNP A1
Background Synonyms:
HNRNPA 1 antibody,Helix destabilizing protein antibody,Helix-destabilizing proteinantibody,Heterogeneous nuclear ribonucleoprotein A1 antibody,Heterogeneous nuclear ribonucleoprotein A1B proteinantibody,Heterogeneous nuclear ribonucleoprotein B2 protein antibody,Heterogeneous nuclear ribonucleoprotein coreprotein A1 antibody,hnRNP A1 antibody,hnRNP core protein A1 antibody,HNRNPA1 antibody,HNRPA1antibody,MGC102835 antibody,Nuclear ribonucleoprotein particle A1 protein antibody,ROA1_HUMANantibody
UniProt P49312
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Application Notes Optimal antibody dilution should be determined by titration, however as a guideline try, Flow Cyt: Use 1µg for 106 cells. IHC-P: Use a concentration of 1 µg/mL. Perform heat mediated antigen retrieval before commencing with IHC staining protocol WB: Use at 1:2000-1:4000. IP: Use at an assay dependent dilution. This antibody does not IP the hnRNP complex
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS, 0.1 % sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C
Supplier Images
anti-Heterogeneous Nuclear Ribonucleoprotein A1 (HNRNPA1) antibody Western blot for anti-hnRNP-A1 on HeLa cell extracts
anti-Heterogeneous Nuclear Ribonucleoprotein A1 (HNRNPA1) antibody (2) anti-Heterogeneous Nuclear Ribonucleoprotein A1 (HNRNPA1) antibody (Image 2)
Product cited in: Matunis, Xing, Dreyfuss: "The hnRNP F protein: unique primary structure, nucleic acid-binding properties, and subcellular localization." in: Nucleic acids research, Vol. 22, Issue 6, pp. 1059-67, 1994 (PubMed).

Kashima, Rao, David et al.: "hnRNP A1 functions with specificity in repression of SMN2 exon 7 splicing." in: Human molecular genetics, Vol. 16, Issue 24, pp. 3149-59, 2007 (PubMed).

Validation Images
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