Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (Biliary Glycoprotein) (CEACAM1) antibody

Antigen: Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (Biliary Glycoprotein) (CEACAM1)

Synonyms: BGP, BGP1, BGPI, 2010110O04Rik, zeh0068, hm:zeh0068, CEACAM8, CEACAM1, MGC142545, Bgp, BGPR, CD66a, Ccam1

Clonality: Monoclonal (GM8G5)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Cell-ELISA (cELISA), Immunohistochemistry (IHC)

Catalog no.: ABIN108725

Additional Information

Alternative name: CEACAM1 (biliary glycoprotein I, BGP, CD66a)

Immunogen: GM8G5 was generated by genetic immunisation of BALB/c mice with an immunisation vector containing the cDNA of the human CEACAM1-A2 domain. The antibody was purified by protein G affinity chromatography from cell culture supernatants and is routinely test

Format: Purified

Isotype: IgG1

Clone: GM8G5

Description: CEACAM1 (BGP) belongs to the carcinoembryonic antigen (CEA) gene family. It is expressed on cells of epithelial and myeloid origin. CEACAM1 expression is downregulated in many tumors indicating a tumor - suppressive function. The antitumor effect may be due to inhibition of tumor angiogenesis, possibly by increased secretion of antiangiogenic molecules from the cells. Like all members of the CEACAM family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by two immunoglobulin constant-like A (A1, A2) and one B domain. While the N, A1 and B domains can also be found in other CEA-family members, the A2 domain of CEACAM1 differs from those found in other CEACAM.

Specificity: GM8G5 recognises CEACAM1 transiently expressed on the cell surface of transfected BOSC cells (Fig. 1) as well as the naturally expressed protein (Fig. 2, kindly provided by Dr. R. Riesenberg, Labor für Tumorimmunologie, Urologische Klinik, Klinikum Grossh

Application Details

Application Notes: GM8G5 has been tested for the use in flow cytometry, cell-ELISA and immunohistology on cryo-sections (Fig. 3, kindly provided by Prof. M. Neumaier, Institut für Klinische Chemie, Universitätsklinikum Mannheim). It is expected that the antibody will be su

Storage: Store the antibody at 4°C. Avoid repeated freezing and thawing.

Research Area: Receptors, Immunology, Cancer, Receptors, Cell/Tissue Markers, Cancer

Restrictions: For Research Use only

Images

Specificity testing of GM8G5. BOSC cells were transiently transfected with expression vectors containing the cDNA of either CEACAM1, 3, 4, 5, 6, 7 or 8. Expression of the constructs was tested with monoclonal antibodies known to recognise the correspondi

GM8G5 on renal cell carcinoma (RCC). Hybridoma supernatant of GM8G5 recognises CEACAM1 expressed on the surface of native RCC (orange curve) and on RCC stimulated with IFN-gamma (red curve). PBS served as negative control (black curve)

Immunohistological staining of colon tissue with GM8G5. Cryosections of colon tissue were stained with 10 ug/ml GM8G5 diluted in PBS containing 2.5% horse serum (B). PBS 2.5% horse serum served as negative control (A). Arrows indicate representative sect

Publications

Publications: Kammerer, Riesenberg, Weiler et al.: "The tumour suppressor gene CEACAM1 is completely but reversibly downregulated in renal cell carcinoma." in: The Journal of pathology, Vol. 204, Issue 3, pp. 258-67, 2004 (PubMed).

Beau, Berger, Servin: "Rotavirus impairs the biosynthesis of brush-border-associated dipeptidyl peptidase IV in human enterocyte-like Caco-2/TC7 cells." in: Cellular microbiology, Vol. 9, Issue 3, pp. 779-89, 2007 (PubMed).

Yoon, Terada, Kita: "CD66b regulates adhesion and activation of human eosinophils." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 12, pp. 8454-62, 2007 (PubMed).

Thomas, Zhu, Schnaar et al.: "Carcinoembryonic antigen and CD44 variant isoforms cooperate to mediate colon carcinoma cell adhesion to E- and L-selectin in shear flow." in: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 15647-55, 2008 (PubMed).

Suemiramoth, Gleizes, Turbica et al.: "Escherichia coli type 1 pili trigger late IL-8 production by neutrophil-like differentiated PLB-985 cells through a Src family kinase- and MAPK-dependent mechanism." in: Journal of leukocyte biology, Vol. 85, Issue 2, pp. 310-21, 2009 (PubMed).