Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (Biliary Glycoprotein) (CEACAM1) antibody

Details for Product No. ABIN108725
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Antigen
Synonyms
zeh0068, hm:zeh0068, CEACAM8, CEACAM1, MGC142545, Bgp, Bgp1, C-CAM, CD66a, Cc1, Cea-1, Cea-7, Cea1, Cea7, Hv-2, Hv2, MHVR, MHVR1, Mhv-1, bb-1, mCEA1, mmCGM1, mmCGM1a, mmCGM2, BGP, BGP1, BGPI, BGPR, Cc ... show more
zeh0068, hm:zeh0068, CEACAM8, CEACAM1, MGC142545, Bgp, Bgp1, C-CAM, CD66a, Cc1, Cea-1, Cea-7, Cea1, Cea7, Hv-2, Hv2, MHVR, MHVR1, Mhv-1, bb-1, mCEA1, mmCGM1, mmCGM1a, mmCGM2, BGP, BGP1, BGPI, BGPR, Ccam1, 2010110O04Rik show less
Reactivity
Human
(109), (55), (36), (17), (3), (1), (1)
Host
Mouse (Murine)
(87), (51), (1), (1), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(12), (11), (11), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application
Flow Cytometry (FACS), Cell-ELISA (cELISA), Immunohistochemistry (Frozen Sections) (IHC (fro))
(62), (60), (54), (35), (26), (24), (17), (15), (9), (4), (2), (1)
Pubmed 9 references available
Quantity 100 µg
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Catalog No. ABIN108725
450.12 $
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Immunogen genetic immunisation with cDNA encoding the extracellular region of human CEACAM1-A2
Clone GM-8G5
Isotype IgG1
Specificity GM8G5 recognises CEACAM1 transiently expressed on the cell surface of transfected BOSC cells as well as the naturally expressed protein (Fig. 2, kindly provided by Dr. R. Riesenberg, Labor für Tumorimmunologie, Urologische Klinik, Klinikum Grossh
Purification Protein G
Alternative Name CEACAM1
Background CEACAM1 (BGP) belongs to the carcinoembryonic antigen (CEA) gene family. CEACAM1 expression is downregulated in many tumors indicating a tumor-suppressive function. GM8G5 was generated by genetic immunisation and can be used to distinguish CEACAM1 from all other CEACAMs.
UniProt P13688
Application Notes Flow cytometry: 1.2 µg/10^6 cells
CELISA: 1:200 - 1:400
ELISA: 1:200 - 1:400
Immunohistology: 10 µg/mL (on cryosections)
For each application a titration should be performed to determine the optimal concentration.
Comment

Synonyms: CD66a, BGP, biliary glycoprotein I

Restrictions For Research Use only
Buffer PBS, pH 7.2
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C
Storage Comment short term: 2 °C - 8 °C, long term: -20 °C
Supplier Images
anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (Biliary Glycoprotein) (CEACAM1) antibody Specificity testing of GM8G5. BOSC cells were transiently transfected with expression vectors containing the cDNA of either CEACAM1, 3, 4, 5, 6, 7 or 8. Expression of the constructs was tested with monoclonal antibodies known to recognise the correspondi
anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (Biliary Glycoprotein) (CEACAM1) antibody (2) GM8G5 on renal cell carcinoma (RCC). Hybridoma supernatant of GM8G5 recognises CEACAM1 expressed on the surface of native RCC (orange curve) and on RCC stimulated with IFN-gamma (red curve). PBS served as negative control (black curve)
anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (Biliary Glycoprotein) (CEACAM1) antibody (3) Immunohistological staining of colon tissue with GM8G5. Cryosections of colon tissue were stained with 10 ug/ml GM8G5 diluted in PBS containing 2.5% horse serum (B). PBS 2.5% horse serum served as negative control (A). Arrows indicate representative sect
Background publications Kammerer, Riesenberg, Weiler et al.: "The tumour suppressor gene CEACAM1 is completely but reversibly downregulated in renal cell carcinoma." in: The Journal of pathology, Vol. 204, Issue 3, pp. 258-67, 2004 (PubMed).

Avadhanula, Rodriguez, Devincenzo et al.: "Respiratory viruses augment the adhesion of bacterial pathogens to respiratory epithelium in a viral species- and cell type-dependent manner." in: Journal of virology, Vol. 80, Issue 4, pp. 1629-36, 2006 (PubMed).

Yoon, Terada, Kita: "CD66b regulates adhesion and activation of human eosinophils." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 12, pp. 8454-62, 2007 (PubMed).

Nittka, Böhm, Zentgraf et al.: "The CEACAM1-mediated apoptosis pathway is activated by CEA and triggers dual cleavage of CEACAM1." in: Oncogene, Vol. 27, Issue 26, pp. 3721-8, 2008 (PubMed).

Thomas, Zhu, Schnaar et al.: "Carcinoembryonic antigen and CD44 variant isoforms cooperate to mediate colon carcinoma cell adhesion to E- and L-selectin in shear flow." in: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 15647-55, 2008 (PubMed).

Béaslas, Torreilles, Casellas et al.: "Transcriptome response of enterocytes to dietary lipids: impact on cell architecture, signaling, and metabolism genes." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 295, Issue 5, pp. G942-52, 2008 (PubMed).

Sémiramoth, Gleizes, Turbica et al.: "Escherichia coli type 1 pili trigger late IL-8 production by neutrophil-like differentiated PLB-985 cells through a Src family kinase- and MAPK-dependent mechanism." in: Journal of leukocyte biology, Vol. 85, Issue 2, pp. 310-21, 2009 (PubMed).

Yue, Goldstein, Hollingsworth et al.: "The prevalence and nature of glycan alterations on specific proteins in pancreatic cancer patients revealed using antibody-lectin sandwich arrays." in: Molecular & cellular proteomics : MCP, Vol. 8, Issue 7, pp. 1697-707, 2009 (PubMed).

General Beau, Berger, Servin: "Rotavirus impairs the biosynthesis of brush-border-associated dipeptidyl peptidase IV in human enterocyte-like Caco-2/TC7 cells." in: Cellular microbiology, Vol. 9, Issue 3, pp. 779-89, 2007 (PubMed).

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