CEACAM1/3/5/6 (CEACAM1/3/5/6) antibody

Details for Product No. ABIN108729
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Mouse (Murine)
Clonality (Clone)
Monoclonal ()
Flow Cytometry (FACS), Cell-ELISA (cELISA), ELISA, Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
(14), (11), (10), (8), (7), (6), (5), (3), (2), (1)
Pubmed 25 references available
Quantity 100 μg
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Catalog No. ABIN108729
375.10 $
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Immunogen immunisation with extracted protein of CEACAM5
Clone D14HD11
Isotype IgG1
Specificity Anti-human CEACAM1,3,4,5,6 (CD66a,c,d,e,CGM7)
Purification Protein G
Alternative Name CEACAM1,3,4,5,6
Background D14HD11 was generated by immunisation with CEA partially purified from a perchloric acid extract from liver metastases of colonic tumors. D14HD11 is a broadly reactive high affinity antibody which reacts with most of the CEACAM molecules (except CEACAM7 and CEACAM8).
UniProt P06731, O75871, P13688, P40199, P40198
Application Notes Flow cytometry: 1.2 µg/10^6 cells
ELISA: 1:200 - 1:400
CELISA: 1:200 - 1:400 Western Blot: 4 µg/mL
Immunofluorescence: 1 µg/106cells
For each application a titration should be performed to determine the optimal concentration.

Synonyms: CD66acde, CGM7

Restrictions For Research Use only
Buffer PBS, pH 7.2
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C
Storage Comment short term: 2 °C - 8 °C, long term: -20 °C
Supplier Images
anti-CEACAM1/3/5/6 (CEACAM1/3/5/6) antibody Specificity testing of D14HD11. BOSC cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3, CEACAM5-CEACAM8 or a recombinant transmembrane-anchored PSG1 fusion protein. Recognition of CEACAM4 was tested
anti-CEACAM1/3/5/6 (CEACAM1/3/5/6) antibody (2) Domain organization of the human CEACAM family. The CEACAM family consists of two subgroups, the CEACAM and the PSG subgroup. CEACAM family members are membranebound by either via a transmembrane domain or a GPI anchor (green arrow) whereas the PSGs are
anti-CEACAM1/3/5/6 (CEACAM1/3/5/6) antibody (3) Immunofluorescence of stable CHO-K1/CEACAM1-transfectant with D14HD11. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.05% saponine and then stained with 50 ug/ml D14HD11 diluted in PBS with 1% BSA (A). Untransfected CHO-K1 parental cells
Background publications Patel, Lee, Ming et al.: "Inside-out signaling promotes dynamic changes in the carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1) oligomeric state to control its cell adhesion properties." in: The Journal of biological chemistry, Vol. 288, Issue 41, pp. 29654-69, 2013 (PubMed).

Johswich, McCaw, Islam et al.: "In vivo adaptation and persistence of Neisseria meningitidis within the nasopharyngeal mucosa." in: PLoS pathogens, Vol. 9, Issue 7, pp. e1003509, 2013 (PubMed).

Voges, Bachmann, Naujoks et al.: "Extracellular IgC2 constant domains of CEACAMs mediate PI3K sensitivity during uptake of pathogens." in: PLoS ONE, Vol. 7, Issue 6, pp. e39908, 2012 (PubMed).

Sarantis, Gray-Owen: "Defining the roles of human carcinoembryonic antigen-related cellular adhesion molecules during neutrophil responses to Neisseria gonorrhoeae." in: Infection and immunity, Vol. 80, Issue 1, pp. 345-58, 2011 (PubMed).

Queval, Nicolas, Beau: "Role of Src kinases in mobilization of glycosylphosphatidylinositol-anchored decay-accelerating factor by Dr fimbria-positive adhering bacteria." in: Infection and immunity, Vol. 79, Issue 7, pp. 2519-34, 2011 (PubMed).

Green, Monk, Partridge et al.: "Cooperative role for tetraspanins in adhesin-mediated attachment of bacterial species to human epithelial cells." in: Infection and immunity, Vol. 79, Issue 6, pp. 2241-9, 2011 (PubMed).

Liévin-Le Moal, Beau, Rougeaux et al.: "Apical expression of human full-length hCEACAM1-4L protein renders the Madin Darby Canine Kidney cells responsive to lipopolysaccharide leading to TLR4-dependent Erk1/2 and p38 MAPK signalling." in: Cellular microbiology, Vol. 13, Issue 5, pp. 764-85, 2011 (PubMed).

Kuespert, Roth, Hauck: "Neisseria meningitidis has two independent modes of recognizing its human receptor CEACAM1." in: PLoS ONE, Vol. 6, Issue 1, pp. e14609, 2011 (PubMed).

Guignot, Hudault, Kansau et al.: "Human decay-accelerating factor and CEACAM receptor-mediated internalization and intracellular lifestyle of Afa/Dr diffusely adhering Escherichia coli in epithelial cells." in: Infection and immunity, Vol. 77, Issue 1, pp. 517-31, 2008 (PubMed).

Lee, Ostrowski, Gray-Owen: "CEACAM1 dynamics during neisseria gonorrhoeae suppression of CD4+ T lymphocyte activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 10, pp. 6827-35, 2008 (PubMed).

Schmitter, Pils, Weibel et al.: "Opa proteins of pathogenic neisseriae initiate Src kinase-dependent or lipid raft-mediated uptake via distinct human carcinoembryonic antigen-related cell adhesion molecule isoforms." in: Infection and immunity, Vol. 75, Issue 8, pp. 4116-26, 2007 (PubMed).

McCaw, Liao, Gray-Owen: "Engulfment of Neisseria gonorrhoeae: revealing distinct processes of bacterial entry by individual carcinoembryonic antigen-related cellular adhesion molecule family receptors." in: Infection and immunity, Vol. 72, Issue 5, pp. 2742-52, 2004 (PubMed).

Boulton, Gray-Owen: "Neisserial binding to CEACAM1 arrests the activation and proliferation of CD4+ T lymphocytes." in: Nature immunology, Vol. 3, Issue 3, pp. 229-36, 2002 (PubMed).

Billker, Popp, Brinkmann et al.: "Distinct mechanisms of internalization of Neisseria gonorrhoeae by members of the CEACAM receptor family involving Rac1- and Cdc42-dependent and -independent pathways." in: The EMBO journal, Vol. 21, Issue 4, pp. 560-71, 2002 (PubMed).

Watt, Teixeira, Zhou et al.: "Homophilic adhesion of human CEACAM1 involves N-terminal domain interactions: structural analysis of the binding site." in: Blood, Vol. 98, Issue 5, pp. 1469-79, 2001 (PubMed).

Schölzel, Zimmermann, Schwarzkopf et al.: "Carcinoembryonic antigen family members CEACAM6 and CEACAM7 are differentially expressed in normal tissues and oppositely deregulated in hyperplastic colorectal polyps and early adenomas." in: The American journal of pathology, Vol. 156, Issue 2, pp. 595-605, 2000 (PubMed).

Dalvi, Birewar, Lokeshwar et al.: "Spontaneous regression of bilateral retrobulbar masses in a newborn? Neuroblastoma." in: Indian pediatrics, Vol. 28, Issue 3, pp. 286-9, 1991 (PubMed).

Siddiqi, Kumar, Kaul et al.: "Salivary nitrate and nitrite concentrations from a sample population of children and adults in high risk area for esophageal and gastric cancers in Kashmir, India." in: Cancer letters, Vol. 64, Issue 2, pp. 133-6, 1992 (PubMed).

Sydow, Schauer, Crozier et al.: "Multiple organ failure in generalized disseminated tuberculosis." in: Respiratory medicine, Vol. 86, Issue 6, pp. 517-9, 1993 (PubMed).

General McCaw, Schneider, Liao et al.: "Immunoreceptor tyrosine-based activation motif phosphorylation during engulfment of Neisseria gonorrhoeae by the neutrophil-restricted CEACAM3 (CD66d) receptor." in: Molecular microbiology, Vol. 49, Issue 3, pp. 623-37, 2003 (PubMed).

Muenzner, Naumann, Meyer et al.: "Pathogenic Neisseria trigger expression of their carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1; previously CD66a) receptor on primary endothelial cells by activating the immediate early response transcription factor, nuclear facto" in: The Journal of biological chemistry, Vol. 276, Issue 26, pp. 24331-40, 2001 (PubMed).

Muenzner, Dehio, Fujiwara et al.: "Carcinoembryonic antigen family receptor specificity of Neisseria meningitidis Opa variants influences adherence to and invasion of proinflammatory cytokine-activated endothelial cells." in: Infection and immunity, Vol. 68, Issue 6, pp. 3601-7, 2000 (PubMed).

Guignot, Peiffer, Bernet-Camard et al.: "Recruitment of CD55 and CD66e brush border-associated glycosylphosphatidylinositol-anchored proteins by members of the Afa/Dr diffusely adhering family of Escherichia coli that infect the human polarized intestinal Caco-2/TC7 cells." in: Infection and immunity, Vol. 68, Issue 6, pp. 3554-63, 2000 (PubMed).

Krop-Watorek, Laskowska, Salwa et al.: "CEA-related proteins on human urothelial cell lines of different transformation grades." in: Cancer letters, Vol. 139, Issue 1, pp. 15-22, 1999 (PubMed).

Jantscheff P, Bottger V, Price M et al.: "Production and characterization of monoclonal antibodies against carcinoembryonic antigen (CEA)." in: Biomed Biochim Acta, Vol. 50, Issue 12, pp. 1261-7, 1991 (PubMed).

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