CEACAM1/3/5/6 (CEACAM1/3/5/6) antibody

Antigen: CEACAM1/3/5/6 (CEACAM1/3/5/6)

Clonality: Monoclonal (D14HD11)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Cell-ELISA (cELISA), Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Catalog no.: ABIN108729

Additional Information

Alternative name: CEACAM1, 3,4,5,6 (CD66acde, CGM7)

Immunogen: immunisation with extracted protein of CEACAM5

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: D14HD11

Description: CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family (13). It consists of seven CEACAM (CEACAM1, CEACAM3-CEACAM8) and 11 pregnancy-specific glyco-protein (PSG1- PSG11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains (Fig. 2). CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor (Fig. 2). CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/ CEACAM5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker (5). The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria (13).

Specificity: Anti-human CEACAM1,3,4,5,6 (CD66a,c,d,e,CGM7)

Application Details

Application Notes: Working dilutions: Flow cytometry: 1.2 ug/10^6 cells, ELISA: 1:200 - 1:400, CELISA: 1:200 - 1:400, Western Blot: 4ug/ml, Immunofluorescence: 1ug/10^6cells

Storage: Store the antibody at 4°C. Avoid repeated freezing and thawing.

Research Area: Receptors, Immunology, Cancer, Receptors, Cell/Tissue Markers, Cancer

Restrictions: For Research Use only

Images

Specificity testing of D14HD11. BOSC cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3, CEACAM5-CEACAM8 or a recombinant transmembrane-anchored PSG1 fusion protein. Recognition of CEACAM4 was tested

Domain organization of the human CEACAM family. The CEACAM family consists of two subgroups, the CEACAM and the PSG subgroup. CEACAM family members are membranebound by either via a transmembrane domain or a GPI anchor (green arrow) whereas the PSGs are

Immunofluorescence of stable CHO-K1/CEACAM1-transfectant with D14HD11. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.05% saponine and then stained with 50 ug/ml D14HD11 diluted in PBS with 1% BSA (A). Untransfected CHO-K1 parental cells

Publications

Publications: Jantscheff P, Bottger V, Price M et al.: "Production and characterization of monoclonal antibodies against carcinoembryonic antigen (CEA)." in: Biomed Biochim Acta, Vol. 50, Issue 12, pp. 1261-7, 1991 (PubMed).

Krop-Watorek, Laskowska, Salwa et al.: "CEA-related proteins on human urothelial cell lines of different transformation grades." in: Cancer letters, Vol. 139, Issue 1, pp. 15-22, 1999 (PubMed).

Guignot, Peiffer, Bernet-Camard et al.: "Recruitment of CD55 and CD66e brush border-associated glycosylphosphatidylinositol-anchored proteins by members of the Afa/Dr diffusely adhering family of Escherichia coli that infect the human polarized intestinal Caco-2/TC7 cells." in: Infection and immunity, Vol. 68, Issue 6, pp. 3554-63, 2000 (PubMed).

Muenzner, Dehio, Fujiwara et al.: "Carcinoembryonic antigen family receptor specificity of Neisseria meningitidis Opa variants influences adherence to and invasion of proinflammatory cytokine-activated endothelial cells." in: Infection and immunity, Vol. 68, Issue 6, pp. 3601-7, 2000 (PubMed).

Muenzner P, Naumann M, Meyer TF et al.: "Pathogenic Neisseria trigger expression of their carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1, previously CD66a) receptor on primary endothelial cells by activating the immediate early response transcription factor, nuclear facto" in: J Biol Chem, Vol. 276, Issue 26, pp. 24331-40, 2001 (PubMed).

Watt SM, Teixeira AM, Zhou GQ et al.: "Homophilic adhesion of human CEACAM1 involves N-terminal domain interactions: structural analysis of the binding site." in: Blood, Vol. 98, Issue 5, pp. 1469-79, 2001 (PubMed).

Billker, Popp, Brinkmann et al.: "Distinct mechanisms of internalization of Neisseria gonorrhoeae by members of the CEACAM receptor family involving Rac1- and Cdc42-dependent and -independent pathways." in: The EMBO journal, Vol. 21, Issue 4, pp. 560-71, 2002 (PubMed).

McCaw, Schneider, Liao et al.: "Immunoreceptor tyrosine-based activation motif phosphorylation during engulfment of Neisseria gonorrhoeae by the neutrophil-restricted CEACAM3 (CD66d) receptor." in: Molecular microbiology, Vol. 49, Issue 3, pp. 623-37, 2003 (PubMed).

Schmitter, Pils, Weibel et al.: "Opa proteins of pathogenic neisseriae initiate Src kinase-dependent or lipid raft-mediated uptake via distinct human carcinoembryonic antigen-related cell adhesion molecule isoforms." in: Infection and immunity, Vol. 75, Issue 8, pp. 4116-26, 2007 (PubMed).

Lee, Ostrowski, Gray-Owen: "CEACAM1 dynamics during neisseria gonorrhoeae suppression of CD4+ T lymphocyte activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 10, pp. 6827-35, 2008 (PubMed).

Guignot, Hudault, Kansau et al.: "Human decay-accelerating factor and CEACAM receptor-mediated internalization and intracellular lifestyle of Afa/Dr diffusely adhering Escherichia coli in epithelial cells." in: Infection and immunity, Vol. 77, Issue 1, pp. 517-31, 2008 (PubMed).

Queval, Nicolas, Beau: "Role of Src kinases in mobilization of glycosylphosphatidylinositol-anchored decay-accelerating factor by Dr fimbria-positive adhering bacteria." in: Infection and immunity, Vol. 79, Issue 7, pp. 2519-34, 2011 (PubMed).