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CEACAM1/3/5/6 (CEACAM1/3/5/6) antibody

Details for Product No. ABIN108729, Supplier: Log in to see
Antigen
Reactivity
Human
11
Host
Mouse
11
Clonality (Clone)
Monoclonal ()
Application
Cell-ELISA (cELISA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
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10
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5
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1
Supplier
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Immunogen immunisation with extracted protein of CEACAM5
Clone D14HD11
Isotype IgG1
Specificity Anti-human CEACAM1,3,4,5,6 (CD66a,c,d,e,CGM7)
Purification Protein G
Alternative Name CEACAM1,3,4,5,6
Background D14HD11 was generated by immunisation with CEA partially purified from a perchloric acid extract from liver metastases of colonic tumors. D14HD11 is a broadly reactive high affinity antibody which reacts with most of the CEACAM molecules (except CEACAM7 and CEACAM8).
UniProt P06731, O75871, P13688, P40199, P40198
Application Notes Flow cytometry: 1.2 µg/10^6 cells
ELISA: 1:200 - 1:400
CELISA: 1:200 - 1:400 Western Blot: 4 µg/mL
Immunofluorescence: 1 µg/106cells
For each application a titration should be performed to determine the optimal concentration.
Comment

Synonyms: CD66acde, CGM7

Restrictions For Research Use only
Buffer PBS, pH 7.2
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C
Storage Comment short term: 2 °C - 8 °C, long term: -20 °C
Supplier Images
 image for anti-CEACAM1/3/5/6 (CEACAM1/3/5/6) antibody (ABIN108729) Specificity testing of D14HD11. BOSC cells were transiently transfected with expressi...
 image for anti-CEACAM1/3/5/6 (CEACAM1/3/5/6) antibody (ABIN108729) Domain organization of the human CEACAM family. The CEACAM family consists of two sub...
 image for anti-CEACAM1/3/5/6 (CEACAM1/3/5/6) antibody (ABIN108729) Immunofluorescence of stable CHO-K1/CEACAM1-transfectant with D14HD11. Cells were fix...
Flow Cytometry (FACS) image for anti-CEACAM1/3/5/6 (CEACAM1/3/5/6) antibody (ABIN108729) BOSC cells were transiently transfected with expression vectors containing either the...
Flow Cytometry (FACS) image for anti-CEACAM1/3/5/6 (CEACAM1/3/5/6) antibody (ABIN108729) FACS analysis of BOSC23 cells using D14HD11. BOSC23 cells were transiently transfecte...
Immunofluorescence (IF) image for anti-CEACAM1/3/5/6 (CEACAM1/3/5/6) antibody (ABIN108729) Immunofluorescence Microscopy of CHO cells using D14HD11. CHO cells were transfected ...
Background publications Patel, Lee, Ming, Rath, Deber, Yip, Rocheleau, Gray-Owen: "Inside-out signaling promotes dynamic changes in the carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1) oligomeric state to control its cell adhesion properties." in: The Journal of biological chemistry, Vol. 288, Issue 41, pp. 29654-69, 2013 (PubMed).

Johswich, McCaw, Islam, Sintsova, Gu, Shively, Gray-Owen: "In vivo adaptation and persistence of Neisseria meningitidis within the nasopharyngeal mucosa." in: PLoS pathogens, Vol. 9, Issue 7, pp. e1003509, 2013 (PubMed).

Voges, Bachmann, Naujoks, Kopp, Hauck: "Extracellular IgC2 constant domains of CEACAMs mediate PI3K sensitivity during uptake of pathogens." in: PLoS ONE, Vol. 7, Issue 6, pp. e39908, 2012 (PubMed).

Queval, Nicolas, Beau: "Role of Src kinases in mobilization of glycosylphosphatidylinositol-anchored decay-accelerating factor by Dr fimbria-positive adhering bacteria." in: Infection and immunity, Vol. 79, Issue 7, pp. 2519-34, 2011 (PubMed).

Sarantis, Gray-Owen: "Defining the roles of human carcinoembryonic antigen-related cellular adhesion molecules during neutrophil responses to Neisseria gonorrhoeae." in: Infection and immunity, Vol. 80, Issue 1, pp. 345-58, 2011 (PubMed).

Green, Monk, Partridge, Morris, Gorringe, Read: "Cooperative role for tetraspanins in adhesin-mediated attachment of bacterial species to human epithelial cells." in: Infection and immunity, Vol. 79, Issue 6, pp. 2241-9, 2011 (PubMed).

Liévin-Le Moal, Beau, Rougeaux, Kansau, Fabrega, Brice, Korotkova, Moseley, Servin: "Apical expression of human full-length hCEACAM1-4L protein renders the Madin Darby Canine Kidney cells responsive to lipopolysaccharide leading to TLR4-dependent Erk1/2 and p38 MAPK signalling." in: Cellular microbiology, Vol. 13, Issue 5, pp. 764-85, 2011 (PubMed).

Kuespert, Roth, Hauck: "Neisseria meningitidis has two independent modes of recognizing its human receptor CEACAM1." in: PLoS ONE, Vol. 6, Issue 1, pp. e14609, 2011 (PubMed).

Guignot, Hudault, Kansau, Chau, Servin: "Human decay-accelerating factor and CEACAM receptor-mediated internalization and intracellular lifestyle of Afa/Dr diffusely adhering Escherichia coli in epithelial cells." in: Infection and immunity, Vol. 77, Issue 1, pp. 517-31, 2008 (PubMed).

Lee, Ostrowski, Gray-Owen: "CEACAM1 dynamics during neisseria gonorrhoeae suppression of CD4+ T lymphocyte activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 10, pp. 6827-35, 2008 (PubMed).

Schmitter, Pils, Weibel, Agerer, Peterson, Buntru, Kopp, Hauck: "Opa proteins of pathogenic neisseriae initiate Src kinase-dependent or lipid raft-mediated uptake via distinct human carcinoembryonic antigen-related cell adhesion molecule isoforms." in: Infection and immunity, Vol. 75, Issue 8, pp. 4116-26, 2007 (PubMed).

McCaw, Liao, Gray-Owen: "Engulfment of Neisseria gonorrhoeae: revealing distinct processes of bacterial entry by individual carcinoembryonic antigen-related cellular adhesion molecule family receptors." in: Infection and immunity, Vol. 72, Issue 5, pp. 2742-52, 2004 (PubMed).

McCaw, Schneider, Liao, Zimmermann, Gray-Owen: "Immunoreceptor tyrosine-based activation motif phosphorylation during engulfment of Neisseria gonorrhoeae by the neutrophil-restricted CEACAM3 (CD66d) receptor." in: Molecular microbiology, Vol. 49, Issue 3, pp. 623-37, 2003 (PubMed).

Billker, Popp, Brinkmann, Wenig, Schneider, Caron, Meyer: "Distinct mechanisms of internalization of Neisseria gonorrhoeae by members of the CEACAM receptor family involving Rac1- and Cdc42-dependent and -independent pathways." in: The EMBO journal, Vol. 21, Issue 4, pp. 560-71, 2002 (PubMed).

Boulton, Gray-Owen: "Neisserial binding to CEACAM1 arrests the activation and proliferation of CD4+ T lymphocytes." in: Nature immunology, Vol. 3, Issue 3, pp. 229-36, 2002 (PubMed).

Muenzner, Naumann, Meyer, Gray-Owen: "Pathogenic Neisseria trigger expression of their carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1, previously CD66a) receptor on primary endothelial cells by activating the immediate early response transcription factor, nuclear facto" in: The Journal of biological chemistry, Vol. 276, Issue 26, pp. 24331-40, 2001 (PubMed).

Watt, Teixeira, Zhou, Doyonnas, Zhang, Grunert, Blumberg, Kuroki, Skubitz, Bates: "Homophilic adhesion of human CEACAM1 involves N-terminal domain interactions: structural analysis of the binding site." in: Blood, Vol. 98, Issue 5, pp. 1469-79, 2001 (PubMed).

Guignot, Peiffer, Bernet-Camard, Lublin, Carnoy, Moseley, Servin: "Recruitment of CD55 and CD66e brush border-associated glycosylphosphatidylinositol-anchored proteins by members of the Afa/Dr diffusely adhering family of Escherichia coli that infect the human polarized intestinal Caco-2/TC7 cells." in: Infection and immunity, Vol. 68, Issue 6, pp. 3554-63, 2000 (PubMed).

Muenzner, Dehio, Fujiwara, Achtman, Meyer, Gray-Owen: "Carcinoembryonic antigen family receptor specificity of Neisseria meningitidis Opa variants influences adherence to and invasion of proinflammatory cytokine-activated endothelial cells." in: Infection and immunity, Vol. 68, Issue 6, pp. 3601-7, 2000 (PubMed).

Schölzel, Zimmermann, Schwarzkopf, Grunert, Rogaczewski, Thompson: "Carcinoembryonic antigen family members CEACAM6 and CEACAM7 are differentially expressed in normal tissues and oppositely deregulated in hyperplastic colorectal polyps and early adenomas." in: The American journal of pathology, Vol. 156, Issue 2, pp. 595-605, 2000 (PubMed).