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CEACAM6 antibody (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (Non-Specific Cross Reacting Antigen))

Details for Product anti-CEACAM6 Antibody No. ABIN108733, Supplier: Log in to see
Antigen
  • CD66c
  • CEAL
  • NCA
Reactivity
Human
82
4
4
Host
Mouse
50
32
Clonality (Clone)
Monoclonal ()
Conjugate
This CEACAM6 antibody is un-conjugated
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Cell-ELISA (cELISA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Western Blotting (WB)
41
32
31
24
18
14
2
1
Supplier
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Available images

Immunogen immunisation with tumor cell lines expressing CEACAM6
Clone 9A6
Isotype IgG1
Specificity Anti-human CEACAM6 (NCA, CD66c)
Purification Protein G
Alternative Name CEACAM6 (CEACAM6 Antibody Abstract)
Background CEACAM6 (NCA) belongs to the carcinoembryonic antigen (CEA) gene family. It is overexpressed in many tumors of epithelial origin. 9A6 was generated by immunisation with tumor cell lines expressing CEACAM6 and can be used to distinguish CEACAM1 from all other CEACAMs.
UniProt P40199
Application Notes Flow cytometry: 1.2 µg/10^6 cells
CELISA: 1:200 - 1:400
ELISA: 1:200 - 1:400 Western blot: 4 µg/mL
Immunohistology: 1-2 µg/10^6 cells (on cryosections)
For each application a titration should be performed to determine the optimal concentration.
Comment

Synonyms: CD66c, NCA

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer PBS, pH 7.2
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C
Storage Comment short term: 2 °C - 8 °C, long term: -20 °C
Supplier Images
 image for anti-CEACAM6 antibody (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (Non-Specific Cross Reacting Antigen)) (ABIN108733) Specificity testing of 9A6. Members of the CEA family were expressed on BOSC cells af...
 image for anti-CEACAM6 antibody (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (Non-Specific Cross Reacting Antigen)) (ABIN108733) Reactivity of FITC-labelled GM-9A6 with polymorph nuclear cells. 2 x 105 polymorph nu...
 image for anti-CEACAM6 antibody (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (Non-Specific Cross Reacting Antigen)) (ABIN108733) FACS analysis of BOSC23 cells using 9A6.BOSC23 cells were transiently transfected wit...
Flow Cytometry (FACS) image for anti-CEACAM6 antibody (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (Non-Specific Cross Reacting Antigen)) (ABIN108733) FACS analysis of BOSC23 cells using 9A6. BOSC23 cells were transiently transfected wi...
Flow Cytometry (FACS) image for anti-CEACAM6 antibody (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (Non-Specific Cross Reacting Antigen)) (ABIN108733) Members of the CEA family were expressed on BOSC cells after transient transfection w...
Background publications Witzens-Harig, Hose, Jünger, Pfirschke, Khandelwal, Umansky, Seckinger, Conrad, Brackertz, Rème, Gueckel, Meißner, Hundemer, Ho, Rossi, Neben, Bernhard, Goldschmidt, Klein, Beckhove: "Tumor cells in multiple myeloma patients inhibit myeloma-reactive T cells through carcinoembryonic antigen-related cell adhesion molecule-6." in: Blood, Vol. 121, Issue 22, pp. 4493-503, 2013 (PubMed).

Denizot, Sivignon, Barreau, Darcha, Chan, Stanners, Hofman, Darfeuille-Michaud, Barnich: "Adherent-invasive Escherichia coli induce claudin-2 expression and barrier defect in CEABAC10 mice and Crohn's disease patients." in: Inflammatory bowel diseases, Vol. 18, Issue 2, pp. 294-304, 2012 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Sarantis, Gray-Owen: "Defining the roles of human carcinoembryonic antigen-related cellular adhesion molecules during neutrophil responses to Neisseria gonorrhoeae." in: Infection and immunity, Vol. 80, Issue 1, pp. 345-58, 2011 (PubMed).

Rolhion, Barnich, Bringer, Glasser, Ranc, Hébuterne, Hofman, Darfeuille-Michaud: "Abnormally expressed ER stress response chaperone Gp96 in CD favours adherent-invasive Escherichia coli invasion." in: Gut, Vol. 59, Issue 10, pp. 1355-62, 2010 (PubMed).

Kanderová, Hrusák, Kalina: "Aberrantly expressed CEACAM6 is involved in the signaling leading to apoptosis of acute lymphoblastic leukemia cells." in: Experimental hematology, Vol. 38, Issue 8, pp. 653-660.e1, 2010 (PubMed).

Singer, Scheffrahn, Kammerer, Suttorp, Ergun, Slevogt: "Deregulation of the CEACAM expression pattern causes undifferentiated cell growth in human lung adenocarcinoma cells." in: PLoS ONE, Vol. 5, Issue 1, pp. e8747, 2010 (PubMed).

Sémiramoth, Gleizes, Turbica, Sandré, Gorges, Kansau, Servin, Chollet-Martin: "Escherichia coli type 1 pili trigger late IL-8 production by neutrophil-like differentiated PLB-985 cells through a Src family kinase- and MAPK-dependent mechanism." in: Journal of leukocyte biology, Vol. 85, Issue 2, pp. 310-21, 2009 (PubMed).

Carvalho, Barnich, Sivignon, Darcha, Chan, Stanners, Darfeuille-Michaud: "Crohn's disease adherent-invasive Escherichia coli colonize and induce strong gut inflammation in transgenic mice expressing human CEACAM." in: The Journal of experimental medicine, Vol. 206, Issue 10, pp. 2179-89, 2009 (PubMed).

Yue, Goldstein, Hollingsworth, Kaul, Brand, Haab: "The prevalence and nature of glycan alterations on specific proteins in pancreatic cancer patients revealed using antibody-lectin sandwich arrays." in: Molecular & cellular proteomics : MCP, Vol. 8, Issue 7, pp. 1697-707, 2009 (PubMed).

Kilic, Oliveira-Ferrer, Neshat-Vahid, Irmak, Obst-Pernberg, Wurmbach, Loges, Kilic, Weil, Lauke, Tilki, Singer, Ergün: "Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1." in: Blood, Vol. 110, Issue 13, pp. 4223-33, 2007 (PubMed).

Chan, Camacho-Leal, Stanners: "Colorectal hyperplasia and dysplasia due to human carcinoembryonic antigen (CEA) family member expression in transgenic mice." in: PLoS ONE, Vol. 2, Issue 12, pp. e1353, 2007 (PubMed).

Kalina, Vaskova, Mejstrikova, Madzo, Trka, Stary, Hrusak: "Myeloid antigens in childhood lymphoblastic leukemia: clinical data point to regulation of CD66c distinct from other myeloid antigens." in: BMC cancer, Vol. 5, pp. 38, 2005 (PubMed).

Muenzner, Naumann, Meyer, Gray-Owen: "Pathogenic Neisseria trigger expression of their carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1, previously CD66a) receptor on primary endothelial cells by activating the immediate early response transcription factor, nuclear facto" in: The Journal of biological chemistry, Vol. 276, Issue 26, pp. 24331-40, 2001 (PubMed).

Watt, Teixeira, Zhou, Doyonnas, Zhang, Grunert, Blumberg, Kuroki, Skubitz, Bates: "Homophilic adhesion of human CEACAM1 involves N-terminal domain interactions: structural analysis of the binding site." in: Blood, Vol. 98, Issue 5, pp. 1469-79, 2001 (PubMed).

Schölzel, Zimmermann, Schwarzkopf, Grunert, Rogaczewski, Thompson: "Carcinoembryonic antigen family members CEACAM6 and CEACAM7 are differentially expressed in normal tissues and oppositely deregulated in hyperplastic colorectal polyps and early adenomas." in: The American journal of pathology, Vol. 156, Issue 2, pp. 595-605, 2000 (PubMed).

Muenzner, Dehio, Fujiwara, Achtman, Meyer, Gray-Owen: "Carcinoembryonic antigen family receptor specificity of Neisseria meningitidis Opa variants influences adherence to and invasion of proinflammatory cytokine-activated endothelial cells." in: Infection and immunity, Vol. 68, Issue 6, pp. 3601-7, 2000 (PubMed).

Krop-Watorek, Laskowska, Salwa, Kłopocki, Grunert, Ugorski: "CEA-related proteins on human urothelial cell lines of different transformation grades." in: Cancer letters, Vol. 139, Issue 1, pp. 15-22, 1999 (PubMed).

Hammarström: "The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues." in: Seminars in cancer biology, Vol. 9, Issue 2, pp. 67-81, 1999 (PubMed).

Thompson, Mössinger, Reichardt, Engels, Beauchemin, Kommoss, von Kleist, Zimmermann: "A polymerase-chain-reaction assay for the specific identification of transcripts encoded by individual carcinoembryonic antigen (CEA)-gene-family members." in: International journal of cancer. Journal international du cancer, Vol. 55, Issue 2, pp. 311-9, 1993 (PubMed).

Product cited in: Negroni, Costanzo, Vitali, Superti, Bertuccini, Tinari, Minelli, Di Nardo, Nuti, Pierdomenico, Cucchiara, Stronati: "Characterization of adherent-invasive Escherichia coli isolated from pediatric patients with inflammatory bowel disease." in: Inflammatory bowel diseases, 2011 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (1:25).