Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (Non-Specific Cross Reacting Antigen) (CEACAM6) antibody

Details for Product No. ABIN108733
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Synonyms CD66c, CEAL, NCA
Mouse (Murine)
(50), (11)
Clonality (Clone)
Monoclonal ()
(5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Flow Cytometry (FACS), Cell-ELISA (cELISA), ELISA, Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
(41), (28), (10), (8), (7), (5), (3), (3), (1), (1), (1), (1)
Pubmed 24 references available
Quantity 100 μg
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Catalog No. ABIN108733
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Immunogen immunisation with tumor cell lines expressing CEACAM6
Clone 9A6
Isotype IgG1
Specificity Anti-human CEACAM6 (NCA, CD66c)
Purification Protein G
Alternative Name CEACAM6
Background CEACAM6 (NCA) belongs to the carcinoembryonic antigen (CEA) gene family. It is overexpressed in many tumors of epithelial origin. 9A6 was generated by immunisation with tumor cell lines expressing CEACAM6 and can be used to distinguish CEACAM1 from all other CEACAMs.
UniProt P40199
Application Notes Flow cytometry: 1.2 µg/10^6 cells
CELISA: 1:200 - 1:400
ELISA: 1:200 - 1:400 Western blot: 4 µg/mL
Immunohistology: 1-2 µg/10^6 cells (on cryosections)
For each application a titration should be performed to determine the optimal concentration.

Synonyms: CD66c, NCA

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer PBS, pH 7.2
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C
Storage Comment short term: 2 °C - 8 °C, long term: -20 °C
Supplier Images
anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (Non-Specific Cross Reacting Antigen) (CEACAM6) antibody Specificity testing of 9A6. Members of the CEA family were expressed on BOSC cells after transient transfection with expression vectors containing either the cDNA of CEACAM1, 5, 6, 7 or 8. Recognition of CEACAM3 and 4 was tested on stably transfected HeLa cells (CEACAM4). Expression of the constructs was confirmed with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 5 and 6: D14HD11, CEACAM7: CAC2, CEACAM8: 80H3 (green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified 9A6 was tested on all CEACAM transfectants. A positive signal was only obtainedwith CEACAM6-expressing cells (red curves).
anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (Non-Specific Cross Reacting Antigen) (CEACAM6) antibody (2) Reactivity of FITC-labelled GM-9A6 with polymorph nuclear cells. 2 x 105 polymorph nuclear cells (PMN) with (green) or without (blue) prior incubation with PMA were reacted for 30 min at 4°C with FITC conjugated GM-9A6 (40 ug/ml) or with an isotype match
anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (Non-Specific Cross Reacting Antigen) (CEACAM6) antibody (3) FACS analysis of BOSC23 cells using 9A6.BOSC23 cells were transiently transfected with an expressionvector encoding either CEACAM6 (red curve) or an irrelevant protein (control transfectant). Binding of 9A6 was detected with aPE conjugated secondary antibody. A positive signal was obtained only with CEACAM6 transfected cells.
Product cited in: Negroni, Costanzo, Vitali et al.: "Characterization of adherent-invasive Escherichia coli isolated from pediatric patients with inflammatory bowel disease." in: Inflammatory bowel diseases, 2011 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (1:25).

Background publications Witzens-Harig, Hose, Jünger et al.: "Tumor cells in multiple myeloma patients inhibit myeloma-reactive T cells through carcinoembryonic antigen-related cell adhesion molecule-6." in: Blood, Vol. 121, Issue 22, pp. 4493-503, 2013 (PubMed).

Sarantis, Gray-Owen: "Defining the roles of human carcinoembryonic antigen-related cellular adhesion molecules during neutrophil responses to Neisseria gonorrhoeae." in: Infection and immunity, Vol. 80, Issue 1, pp. 345-58, 2011 (PubMed).

Denizot, Sivignon, Barreau et al.: "Adherent-invasive Escherichia coli induce claudin-2 expression and barrier defect in CEABAC10 mice and Crohn's disease patients." in: Inflammatory bowel diseases, Vol. 18, Issue 2, pp. 294-304, 2012 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Rolhion, Barnich, Bringer et al.: "Abnormally expressed ER stress response chaperone Gp96 in CD favours adherent-invasive Escherichia coli invasion." in: Gut, Vol. 59, Issue 10, pp. 1355-62, 2010 (PubMed).

Kanderová, Hrusák, Kalina: "Aberrantly expressed CEACAM6 is involved in the signaling leading to apoptosis of acute lymphoblastic leukemia cells." in: Experimental hematology, Vol. 38, Issue 8, pp. 653-660.e1, 2010 (PubMed).

Singer, Scheffrahn, Kammerer et al.: "Deregulation of the CEACAM expression pattern causes undifferentiated cell growth in human lung adenocarcinoma cells." in: PLoS ONE, Vol. 5, Issue 1, pp. e8747, 2010 (PubMed).

Yue, Goldstein, Hollingsworth et al.: "The prevalence and nature of glycan alterations on specific proteins in pancreatic cancer patients revealed using antibody-lectin sandwich arrays." in: Molecular & cellular proteomics : MCP, Vol. 8, Issue 7, pp. 1697-707, 2009 (PubMed).

Sémiramoth, Gleizes, Turbica et al.: "Escherichia coli type 1 pili trigger late IL-8 production by neutrophil-like differentiated PLB-985 cells through a Src family kinase- and MAPK-dependent mechanism." in: Journal of leukocyte biology, Vol. 85, Issue 2, pp. 310-21, 2009 (PubMed).

Chan, Camacho-Leal, Stanners: "Colorectal hyperplasia and dysplasia due to human carcinoembryonic antigen (CEA) family member expression in transgenic mice." in: PLoS ONE, Vol. 2, Issue 12, pp. e1353, 2007 (PubMed).

Kilic, Oliveira-Ferrer, Neshat-Vahid et al.: "Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1." in: Blood, Vol. 110, Issue 13, pp. 4223-33, 2007 (PubMed).

Kalina, Vaskova, Mejstrikova et al.: "Myeloid antigens in childhood lymphoblastic leukemia: clinical data point to regulation of CD66c distinct from other myeloid antigens." in: BMC cancer, Vol. 5, pp. 38, 2005 (PubMed).

Eddie, Cameron, Leeton et al.: "Ovarian relaxin is not essential for dilatation of cervix." in: Lancet, Vol. 336, Issue 8709, pp. 243, 1990 (PubMed).

Sharma, Kulkarni, Nayar: "Effect of NMDA-receptor ligands on neocortical and hippocampal EEG activity of rat brain." in: Indian journal of experimental biology, Vol. 29, Issue 8, pp. 744-50, 1992 (PubMed).

Sydow, Schauer, Crozier et al.: "Multiple organ failure in generalized disseminated tuberculosis." in: Respiratory medicine, Vol. 86, Issue 6, pp. 517-9, 1993 (PubMed).

General Carvalho, Barnich, Sivignon et al.: "Crohn's disease adherent-invasive Escherichia coli colonize and induce strong gut inflammation in transgenic mice expressing human CEACAM." in: The Journal of experimental medicine, Vol. 206, Issue 10, pp. 2179-89, 2009 (PubMed).

Watt, Teixeira, Zhou et al.: "Homophilic adhesion of human CEACAM1 involves N-terminal domain interactions: structural analysis of the binding site." in: Blood, Vol. 98, Issue 5, pp. 1469-79, 2001 (PubMed).

Muenzner, Naumann, Meyer et al.: "Pathogenic Neisseria trigger expression of their carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1; previously CD66a) receptor on primary endothelial cells by activating the immediate early response transcription factor, nuclear facto" in: The Journal of biological chemistry, Vol. 276, Issue 26, pp. 24331-40, 2001 (PubMed).

Muenzner, Dehio, Fujiwara et al.: "Carcinoembryonic antigen family receptor specificity of Neisseria meningitidis Opa variants influences adherence to and invasion of proinflammatory cytokine-activated endothelial cells." in: Infection and immunity, Vol. 68, Issue 6, pp. 3601-7, 2000 (PubMed).

Schölzel, Zimmermann, Schwarzkopf et al.: "Carcinoembryonic antigen family members CEACAM6 and CEACAM7 are differentially expressed in normal tissues and oppositely deregulated in hyperplastic colorectal polyps and early adenomas." in: The American journal of pathology, Vol. 156, Issue 2, pp. 595-605, 2000 (PubMed).

Krop-Watorek, Laskowska, Salwa et al.: "CEA-related proteins on human urothelial cell lines of different transformation grades." in: Cancer letters, Vol. 139, Issue 1, pp. 15-22, 1999 (PubMed).

Thompson, Mössinger, Reichardt et al.: "A polymerase-chain-reaction assay for the specific identification of transcripts encoded by individual carcinoembryonic antigen (CEA)-gene-family members." in: International journal of cancer. Journal international du cancer, Vol. 55, Issue 2, pp. 311-9, 1993 (PubMed).

Daniel, Nagel, Johnson et al.: "Determination of the specificities of monoclonal antibodies recognizing members of the CEA family using a panel of transfectants." in: International journal of cancer. Journal international du cancer, Vol. 55, Issue 2, pp. 303-10, 1993 (PubMed).

Göttlinger, Lobo, Grimm et al.: "Biochemical characterization and tissue distribution of the Cora antigen, a cell surface glycoprotein differentially expressed on malignant and benign gastrointestinal epithelia." in: Cancer research, Vol. 48, Issue 8, pp. 2198-203, 1988 (PubMed).

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