You are viewing an incomplete version of our website. Please click to reload the website as full version.

Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (Non-Specific Cross Reacting Antigen) (CEACAM6) antibody

Details for Product No. ABIN108733, Supplier: Log in to see
  • CD66c
  • CEAL
  • NCA
Clonality (Clone)
Monoclonal ()
Cell-ELISA (cELISA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Western Blotting (WB)
Log in to see
Supplier Product No.
Log in to see

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Available images

Immunogen immunisation with tumor cell lines expressing CEACAM6
Clone 9A6
Isotype IgG1
Specificity Anti-human CEACAM6 (NCA, CD66c)
Purification Protein G
Alternative Name CEACAM6 (CEACAM6 Antibody Abstract)
Background CEACAM6 (NCA) belongs to the carcinoembryonic antigen (CEA) gene family. It is overexpressed in many tumors of epithelial origin. 9A6 was generated by immunisation with tumor cell lines expressing CEACAM6 and can be used to distinguish CEACAM1 from all other CEACAMs.
UniProt P40199
Application Notes Flow cytometry: 1.2 µg/10^6 cells
CELISA: 1:200 - 1:400
ELISA: 1:200 - 1:400 Western blot: 4 µg/mL
Immunohistology: 1-2 µg/10^6 cells (on cryosections)
For each application a titration should be performed to determine the optimal concentration.

Synonyms: CD66c, NCA

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer PBS, pH 7.2
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C
Storage Comment short term: 2 °C - 8 °C, long term: -20 °C
Supplier Images
 image for anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (Non-Specific Cross Reacting Antigen) (CEACAM6) antibody (ABIN108733) Specificity testing of 9A6. Members of the CEA family were expressed on BOSC cells af...
 image for anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (Non-Specific Cross Reacting Antigen) (CEACAM6) antibody (ABIN108733) Reactivity of FITC-labelled GM-9A6 with polymorph nuclear cells. 2 x 105 polymorph nu...
 image for anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (Non-Specific Cross Reacting Antigen) (CEACAM6) antibody (ABIN108733) FACS analysis of BOSC23 cells using 9A6.BOSC23 cells were transiently transfected wit...
Flow Cytometry (FACS) image for anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (Non-Specific Cross Reacting Antigen) (CEACAM6) antibody (ABIN108733) FACS analysis of BOSC23 cells using 9A6. BOSC23 cells were transiently transfected wi...
Flow Cytometry (FACS) image for anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (Non-Specific Cross Reacting Antigen) (CEACAM6) antibody (ABIN108733) Members of the CEA family were expressed on BOSC cells after transient transfection w...
Product cited in: Negroni, Costanzo, Vitali et al.: "Characterization of adherent-invasive Escherichia coli isolated from pediatric patients with inflammatory bowel disease." in: Inflammatory bowel diseases, 2011 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (1:25).

Background publications Witzens-Harig, Hose, Jünger et al.: "Tumor cells in multiple myeloma patients inhibit myeloma-reactive T cells through carcinoembryonic antigen-related cell adhesion molecule-6." in: Blood, Vol. 121, Issue 22, pp. 4493-503, 2013 (PubMed).

Sarantis, Gray-Owen: "Defining the roles of human carcinoembryonic antigen-related cellular adhesion molecules during neutrophil responses to Neisseria gonorrhoeae." in: Infection and immunity, Vol. 80, Issue 1, pp. 345-58, 2011 (PubMed).

Denizot, Sivignon, Barreau et al.: "Adherent-invasive Escherichia coli induce claudin-2 expression and barrier defect in CEABAC10 mice and Crohn's disease patients." in: Inflammatory bowel diseases, Vol. 18, Issue 2, pp. 294-304, 2012 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Rolhion, Barnich, Bringer et al.: "Abnormally expressed ER stress response chaperone Gp96 in CD favours adherent-invasive Escherichia coli invasion." in: Gut, Vol. 59, Issue 10, pp. 1355-62, 2010 (PubMed).

Kanderová, Hrusák, Kalina: "Aberrantly expressed CEACAM6 is involved in the signaling leading to apoptosis of acute lymphoblastic leukemia cells." in: Experimental hematology, Vol. 38, Issue 8, pp. 653-660.e1, 2010 (PubMed).

Singer, Scheffrahn, Kammerer et al.: "Deregulation of the CEACAM expression pattern causes undifferentiated cell growth in human lung adenocarcinoma cells." in: PLoS ONE, Vol. 5, Issue 1, pp. e8747, 2010 (PubMed).

Carvalho, Barnich, Sivignon et al.: "Crohn's disease adherent-invasive Escherichia coli colonize and induce strong gut inflammation in transgenic mice expressing human CEACAM." in: The Journal of experimental medicine, Vol. 206, Issue 10, pp. 2179-89, 2009 (PubMed).

Yue, Goldstein, Hollingsworth et al.: "The prevalence and nature of glycan alterations on specific proteins in pancreatic cancer patients revealed using antibody-lectin sandwich arrays." in: Molecular & cellular proteomics : MCP, Vol. 8, Issue 7, pp. 1697-707, 2009 (PubMed).

Sémiramoth, Gleizes, Turbica et al.: "Escherichia coli type 1 pili trigger late IL-8 production by neutrophil-like differentiated PLB-985 cells through a Src family kinase- and MAPK-dependent mechanism." in: Journal of leukocyte biology, Vol. 85, Issue 2, pp. 310-21, 2009 (PubMed).

Chan, Camacho-Leal, Stanners: "Colorectal hyperplasia and dysplasia due to human carcinoembryonic antigen (CEA) family member expression in transgenic mice." in: PLoS ONE, Vol. 2, Issue 12, pp. e1353, 2007 (PubMed).

Kilic, Oliveira-Ferrer, Neshat-Vahid et al.: "Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1." in: Blood, Vol. 110, Issue 13, pp. 4223-33, 2007 (PubMed).

Kalina, Vaskova, Mejstrikova et al.: "Myeloid antigens in childhood lymphoblastic leukemia: clinical data point to regulation of CD66c distinct from other myeloid antigens." in: BMC cancer, Vol. 5, pp. 38, 2005 (PubMed).

Watt, Teixeira, Zhou et al.: "Homophilic adhesion of human CEACAM1 involves N-terminal domain interactions: structural analysis of the binding site." in: Blood, Vol. 98, Issue 5, pp. 1469-79, 2001 (PubMed).

Muenzner, Naumann, Meyer et al.: "Pathogenic Neisseria trigger expression of their carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1, previously CD66a) receptor on primary endothelial cells by activating the immediate early response transcription factor, nuclear facto" in: The Journal of biological chemistry, Vol. 276, Issue 26, pp. 24331-40, 2001 (PubMed).

Muenzner, Dehio, Fujiwara et al.: "Carcinoembryonic antigen family receptor specificity of Neisseria meningitidis Opa variants influences adherence to and invasion of proinflammatory cytokine-activated endothelial cells." in: Infection and immunity, Vol. 68, Issue 6, pp. 3601-7, 2000 (PubMed).

Hammarström: "The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues." in: Seminars in cancer biology, Vol. 9, Issue 2, pp. 67-81, 1999 (PubMed).

Daniel, Nagel, Johnson et al.: "Determination of the specificities of monoclonal antibodies recognizing members of the CEA family using a panel of transfectants." in: International journal of cancer. Journal international du cancer, Vol. 55, Issue 2, pp. 303-10, 1993 (PubMed).

Göttlinger, Lobo, Grimm et al.: "Biochemical characterization and tissue distribution of the Cora antigen, a cell surface glycoprotein differentially expressed on malignant and benign gastrointestinal epithelia." in: Cancer research, Vol. 48, Issue 8, pp. 2198-203, 1988 (PubMed).

Eddie, Cameron, Leeton et al.: "Ovarian relaxin is not essential for dilatation of cervix." in: Lancet, Vol. 336, Issue 8709, pp. 243, 1990 (PubMed).

Sharma, Kulkarni, Nayar: "Effect of NMDA-receptor ligands on neocortical and hippocampal EEG activity of rat brain." in: Indian journal of experimental biology, Vol. 29, Issue 8, pp. 744-50, 1992 (PubMed).

Sydow, Schauer, Crozier et al.: "Multiple organ failure in generalized disseminated tuberculosis." in: Respiratory medicine, Vol. 86, Issue 6, pp. 517-9, 1993 (PubMed).