Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (Non-Specific Cross Reacting Antigen) (CEACAM6) antibody

Antigen: Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (Non-Specific Cross Reacting Antigen) (CEACAM6)

Synonyms: NCA, CEAL, CD66c

Clonality: Monoclonal (9A6)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Cell-ELISA (cELISA), Western Blotting (WB), ELISA

Catalog no.: ABIN108733

Additional Information

Alternative name: CEACAM6 (CEA-related cell adhesion molecule 5, non-specific cross-reacting antigen (NCA), CD66c)

Swiss-Prot: P40199

Immunogen: immunisation with tumor cell lines expressing CEACAM6

Format: Purified

Isotype: IgG1

Clone: 9A6

Description: CEA-related cell adhesion molecule 6 (CEACAM6, NCA) belongs to the carcinoembryonic antigen (CEA) gene family (13). It encodes a glycosyl phosphatidyl inositol (GPI)-linked glycoprotein with a Mr of 90,000 which is strongly expressed on epithelial cells of the fetal and adult gastrointestinal tract, epithelia of glandular tissues, squamous epithelial cell of the tongue, esophagus and cervix as well as on granulocytes (Fig. 2, Ref. 4, 10). CEACAM6 expression is upregulated in many adenocarcinomas (4) and leukemias (5). Like all members of the CEA family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by one immunoglobulin constant-like A and B domain.

Specificity: Anti-human CEACAM6 (NCA, CD66c)

Application Details

Application Notes: 26/5/1 has been tested for the use in flow cytometry (1.2 ug/10^6 cells), cell-ELISA (1:200), ELISA (1:200 - 1:400), Western blots (4ug/ml) and immunohistology on cryosections (1-2 ug/10^6 cells).

Concentration: 1mg/ml

Purification: Protein G

Buffer: phosphate buffered saline, pH 7.2

Storage: Short term: 2°C - 8°C. Long term: -20°C (avoid repeated freezing and thawing)

Research Area: Receptors, Immunology, Cancer, Receptors, Cell/Tissue Markers, Cancer

Restrictions: For Research Use only

Images

Specificity testing of 9A6. Members of the CEA family were expressed on BOSC cells after transient transfection with expression vectors containing either the cDNA of CEACAM1, 5, 6, 7 or 8. Recognition of CEACAM3 and 4 was tested on stably transfected HeL

Reactivity of FITC-labelled GM-9A6 with polymorph nuclear cells. 2 x 105 polymorph nuclear cells (PMN) with (green) or without (blue) prior incubation with PMA were reacted for 30 min at 4°C with FITC conjugated GM-9A6 (40 ug/ml) or with an isotype match

FACS analysis of BOSC23 cells using 9A6.BOSC23 cells were transiently transfected with an expressionvector encoding either CEACAM6 (red curve) or an irrelevant protein (control transfectant). Binding of 9A6 was detected with aPE conjugated secondary anti

Publications

Publications: Gottlinger HG, Lobo FM, Grimm TW et al.: "Biochemical characterization and tissue distribution of the Cora antigen, a cell surface glycoprotein differentially expressed on malignant and benign gastrointestinal epithelia." in: Cancer Res, Vol. 48, Issue 8, pp. 2198-203, 1988 (PubMed).

Daniel S, Nagel G, Johnson JP et al.: "Determination of the specificities of monoclonal antibodies recognizing members of the CEA family using a panel of transfectants." in: Int J Cancer, Vol. 55, Issue 2, pp. 303-10, 1993 (PubMed).

Thompson, Moessinger, Reichardt et al.: "A polymerase-chain-reaction assay for the specific identification of transcripts encoded by individual carcinoembryonic antigen (CEA)-gene-family members." in: International journal of cancer. Journal international du cancer, Vol. 55, Issue 2, pp. 311-9, 1993 (PubMed).

Krop-Watorek, Laskowska, Salwa et al.: "CEA-related proteins on human urothelial cell lines of different transformation grades." in: Cancer letters, Vol. 139, Issue 1, pp. 15-22, 1999 (PubMed).

Schoelzel, Zimmermann, Schwarzkopf et al.: "Carcinoembryonic antigen family members CEACAM6 and CEACAM7 are differentially expressed in normal tissues and oppositely deregulated in hyperplastic colorectal polyps and early adenomas." in: The American journal of pathology, Vol. 156, Issue 2, pp. 595-605, 2000 (PubMed).

Muenzner, Dehio, Fujiwara et al.: "Carcinoembryonic antigen family receptor specificity of Neisseria meningitidis Opa variants influences adherence to and invasion of proinflammatory cytokine-activated endothelial cells." in: Infection and immunity, Vol. 68, Issue 6, pp. 3601-7, 2000 (PubMed).

Muenzner P, Naumann M, Meyer TF et al.: "Pathogenic Neisseria trigger expression of their carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1, previously CD66a) receptor on primary endothelial cells by activating the immediate early response transcription factor, nuclear facto" in: J Biol Chem, Vol. 276, Issue 26, pp. 24331-40, 2001 (PubMed).

Watt SM, Teixeira AM, Zhou GQ et al.: "Homophilic adhesion of human CEACAM1 involves N-terminal domain interactions: structural analysis of the binding site." in: Blood, Vol. 98, Issue 5, pp. 1469-79, 2001 (PubMed).

Kilic, Oliveira-Ferrer, Neshat-Vahid et al.: "Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1." in: Blood, Vol. 110, Issue 13, pp. 4223-33, 2007 (PubMed).

Suemiramoth, Gleizes, Turbica et al.: "Escherichia coli type 1 pili trigger late IL-8 production by neutrophil-like differentiated PLB-985 cells through a Src family kinase- and MAPK-dependent mechanism." in: Journal of leukocyte biology, Vol. 85, Issue 2, pp. 310-21, 2009 (PubMed).

Carvalho, Barnich, Sivignon et al.: "Crohn's disease adherent-invasive Escherichia coli colonize and induce strong gut inflammation in transgenic mice expressing human CEACAM." in: The Journal of experimental medicine, Vol. 206, Issue 10, pp. 2179-89, 2009 (PubMed).

Rolhion, Barnich, Bringer et al.: "Abnormally expressed ER stress response chaperone Gp96 in CD favours adherent-invasive Escherichia coli invasion." in: Gut, 2010 (PubMed).

Denizot, Sivignon, Barreau et al.: "Adherent-invasive Escherichia coli induce claudin-2 expression and barrier defect in CEABAC10 mice and crohn's disease patients." in: Inflammatory bowel diseases, 2011 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Negroni, Costanzo, Vitali et al.: "Characterization of adherent-invasive Escherichia coli isolated from pediatric patients with inflammatory bowel disease." in: Inflammatory bowel diseases, 2011 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (1:25).