T (AA 257-309) antibody

Details for Product No. ABIN1098136
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Antigen
Epitope
AA 257-309
(7), (7), (3), (2), (1), (1), (1), (1)
Reactivity
Human
(21), (1)
Host
Mouse
(13), (12)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
(17), (12), (3), (3), (1), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Immunogen Purified recombinant fragment of human T (AA 257-309 ) expressed in E. coli.
Clone 1H9A2
Isotype IgG1
Purification Purified
Background Synonyms:
TFT
The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. ,,,,,
Molecular Weight 47.4 kDa
Gene ID 6862
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
anti-T (AA 257-309) antibody Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 ng), Blue: ...
anti-T (AA 257-309) antibody (2) Figure 4: Flow cytometric analysis of HeLa cells using T mouse mAb (green) and negati...
anti-T (AA 257-309) antibody (3) Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues u...
anti-T (AA 257-309) antibody (4) Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues usi...
anti-T (AA 257-309) antibody (5) Figure 3: Western blot analysis using T mouse mAb against Raji (1), and Jurkat (2) ce...
anti-T (AA 257-309) antibody (6) Figure 1: Western blot analysis using T mAb against human T recombinant protein. (Exp...
anti-T (AA 257-309) antibody (7) Figure 2: Western blot analysis using T mAb against HEK293 (1) and T (AA: 257-309) -h...
Product cited in: Shen, Li, Yang et al.: "Classic chordoma coexisting with benign notochordal cell rest demonstrating different immunohistological expression patterns of brachyury and galectin-3." in: Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia, Vol. 18, Issue 1, pp. 96-9, 2010 (PubMed).

Tirabosco, Mangham, Rosenberg et al.: "Brachyury expression in extra-axial skeletal and soft tissue chordomas: a marker that distinguishes chordoma from mixed tumor/myoepithelioma/parachordoma in soft tissue." in: The American journal of surgical pathology, Vol. 32, Issue 4, pp. 572-80, 2008 (PubMed).

Validation Images
Catalog No. ABIN1098136
430.10 $
Plus shipping costs $45.00
Quantity
Price
0.1 mg
430.10 $

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