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T (AA 257-309) antibody

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AA 257-309
(9), (8), (3), (2), (2), (2), (1), (1)
(23), (3)
(12), (11)
Clonality (Clone)
Monoclonal ()
(1), (1), (1), (1), (1), (1)
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(20), (11), (7), (3), (1)
Pubmed 2 references available
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Quantity 0.1 mg
Shipping to United States ( )
Availability Will be delivered in 3 to 4 Business Days
Immunogen Purified recombinant fragment of human T (AA 257-309 ) expressed in E. coli.
Clone 1H9A2
Isotype IgG1
Purification Purified
Background The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. , , , , ,
Molecular Weight 47.4 kDa
Gene ID 6862
HGNC 6862
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
ELISA image for anti-T (AA 257-309) antibody (ABIN1098136) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-T (AA 257-309) antibody (ABIN1098136) Figure 2: Flow cytometric analysis of HeLa cells using T mouse mAb (green) and negati...
Immunohistochemistry (IHC) image for anti-T (AA 257-309) antibody (ABIN1098136) Figure 3: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues u...
Immunohistochemistry (IHC) image for anti-T (AA 257-309) antibody (ABIN1098136) Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues usi...
Western Blotting (WB) image for anti-T (AA 257-309) antibody (ABIN1098136) Figure 5: Western blot analysis using T mouse mAb against Raji (1), and Jurkat (2) ce...
Western Blotting (WB) image for anti-T (AA 257-309) antibody (ABIN1098136) Figure 6: Western blot analysis using T mAb against human T recombinant protein. (Exp...
Western Blotting (WB) image for anti-T (AA 257-309) antibody (ABIN1098136) Figure 7: Western blot analysis using T mAb against HEK293 (1) and T (AA: 257-309) -h...
Product cited in: Shen, Li, Yang et al.: "Classic chordoma coexisting with benign notochordal cell rest demonstrating different immunohistological expression patterns of brachyury and galectin-3." in: Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia, Vol. 18, Issue 1, pp. 96-9, 2010 (PubMed).

Tirabosco, Mangham, Rosenberg et al.: "Brachyury expression in extra-axial skeletal and soft tissue chordomas: a marker that distinguishes chordoma from mixed tumor/myoepithelioma/parachordoma in soft tissue." in: The American journal of surgical pathology, Vol. 32, Issue 4, pp. 572-80, 2008 (PubMed).

Catalog No. ABIN1098136
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