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T (AA 257-309) antibody

Details for Product No. ABIN1098136, Supplier: Log in to see
Antigen
Epitope
AA 257-309
9
8
3
2
2
1
1
1
Reactivity
Human
22
2
Host
Mouse
11
11
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
19
11
7
3
1
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Immunogen Purified recombinant fragment of human T (AA 257-309 ) expressed in E. coli.
Clone 1H9A2
Isotype IgG1
Purification Purified
Background The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. , , , , ,
Molecular Weight 47.4 kDa
Gene ID 6862
HGNC 6862
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
ELISA image for anti-T (AA 257-309) antibody (ABIN1098136) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-T (AA 257-309) antibody (ABIN1098136) Figure 2: Flow cytometric analysis of HeLa cells using T mouse mAb (green) and negati...
Immunohistochemistry (IHC) image for anti-T (AA 257-309) antibody (ABIN1098136) Figure 3: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues u...
Immunohistochemistry (IHC) image for anti-T (AA 257-309) antibody (ABIN1098136) Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues usi...
Western Blotting (WB) image for anti-T (AA 257-309) antibody (ABIN1098136) Figure 5: Western blot analysis using T mouse mAb against Raji (1), and Jurkat (2) ce...
Western Blotting (WB) image for anti-T (AA 257-309) antibody (ABIN1098136) Figure 6: Western blot analysis using T mAb against human T recombinant protein. (Exp...
Western Blotting (WB) image for anti-T (AA 257-309) antibody (ABIN1098136) Figure 7: Western blot analysis using T mAb against HEK293 (1) and T (AA: 257-309) -h...
Product cited in: Shen, Li, Yang et al.: "Classic chordoma coexisting with benign notochordal cell rest demonstrating different immunohistological expression patterns of brachyury and galectin-3." in: Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia, Vol. 18, Issue 1, pp. 96-9, 2010 (PubMed).

Tirabosco, Mangham, Rosenberg et al.: "Brachyury expression in extra-axial skeletal and soft tissue chordomas: a marker that distinguishes chordoma from mixed tumor/myoepithelioma/parachordoma in soft tissue." in: The American journal of surgical pathology, Vol. 32, Issue 4, pp. 572-80, 2008 (PubMed).