T (AA 257-309) antibody

Details for Product No. ABIN1098136
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Antigen
Epitope
AA 257-309
(2), (1), (1), (1), (1)
Reactivity
Human
(10), (1)
Host
Mouse
(6), (4)
Clonality (Clone)
Monoclonal ()
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
(6), (6), (2), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN1098136
396.00 $
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Immunogen Purified recombinant fragment of human T (AA 257-309 ) expressed in E. coli.
Clone 1H9A2
Isotype IgG1
Purification Purified
Background Synonyms:
TFT
The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. ,,,,,
Molecular Weight 47.4 kDa
Gene ID 6862
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
anti-T (AA 257-309) antibody Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 ng), Blue: Antigen (100 ng)
anti-T (AA 257-309) antibody (2) Figure 4: Flow cytometric analysis of HeLa cells using T mouse mAb (green) and negative control (red) .
anti-T (AA 257-309) antibody (3) Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using T mouse mAb with DAB staining.
anti-T (AA 257-309) antibody (4) Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using T mouse mAb with DAB staining.
anti-T (AA 257-309) antibody (5) Figure 3: Western blot analysis using T mouse mAb against Raji (1), and Jurkat (2) cell lysate.
anti-T (AA 257-309) antibody (6) Figure 1: Western blot analysis using T mAb against human T recombinant protein. (Expected MW is 31.2 kDa)
anti-T (AA 257-309) antibody (7) Figure 2: Western blot analysis using T mAb against HEK293 (1) and T (AA: 257-309) -hIgGFc transfected HEK293 (2) cell lysate.
Product cited in: Tirabosco, Mangham, Rosenberg et al.: "Brachyury expression in extra-axial skeletal and soft tissue chordomas: a marker that distinguishes chordoma from mixed tumor/myoepithelioma/parachordoma in soft tissue." in: The American journal of surgical pathology, Vol. 32, Issue 4, pp. 572-80, 2008 (PubMed).

Shen, Li, Yang et al.: "Classic chordoma coexisting with benign notochordal cell rest demonstrating different immunohistological expression patterns of brachyury and galectin-3." in: Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia, Vol. 18, Issue 1, pp. 96-9, 2010 (PubMed).

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