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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Keratin 20 (KRT20) antibody (FITC)
|Synonyms||K20, CD20, CK20, KRT21, MGC35423, 9030623C06Rik, Krt21, KRT20, MGC152269|
Human, Pig (Porcine), Rat (Rattus)
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|7 references available|
|Quantity||0.25 mL (0.2 mg/ml)|
|Price||594.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 6 to 8 Business Days|
|Alternative name||Cytokeratin 20|
|Immunogen||Electrophoretically purified keratin K 20 from human intestinal mucosa.|
|Cross-Reactivity||Human, Pig (Porcine), Rat (Rattus)|
|Description||Cytokeratin 20 is a 46 kDa intermediate filament protein whose expression is restrictedprimarily to gastric and intestinal epithelium, urothelium, and Merkel cells. This antibodyis highly specific to cytokeratin 20 and shows no cross-reactivity with other IFPs. Cytokeratin 20 is a unique type I keratin that is expressed in adenocarcinomas of the colonstomach, pancreas and bile system. It is also expressed in mucinous ovarian tumors,transitional cell carcinomas of the urinary tract, and Merkel cell carcinomas. This antibody is essentially non-reactive in squamous cell carcinomas andadenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of theovary, and small cell carcinomas. This antibody is often used in conjunction with CK7 andother antibodies to distinguishing colon carcinomas (CK20+), from ovarian, pulmonary,and breast carcinomas.|
|Characteristics||Synonyms: Cytokeratin 20, Keratin-20, KRT20, CK20, K20, Protein IT, Keratin type I cytoskeletal 20|
|Application Notes||FACS. Cytological Material. Immunohistochemistry on Frozen Sections. . Immunohistochemistry on Paraffin sections (Only after microwave treatment). Working Dilution: 1/10-1/20 in PBS, PH 7.4 for Immunohistochemistry. Incubation Time: 1h at RT or over night at 2-8°C. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.|
|Storage||Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Protect from light. Shelf life: one year from despatch.|
|Research Area||Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix, Cancer|
|Restrictions||For Research Use only|
|Mouse anti Cytokeratin-20 (KRT20) KS20.10|
Moll, Loewe, Laufer et al.: "Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies." in: The American journal of pathology, Vol. 140, Issue 2, pp. 427-47, 1992 (PubMed).
Moll, Schiller, Franke: "Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns." in: The Journal of cell biology, Vol. 111, Issue 2, pp. 567-80, 1990 (PubMed).
Moll, Moll: "Comparative cytokeratin analysis of sweat gland ducts and eccrine poromas." in: Archives of dermatological research, Vol. 283, Issue 5, pp. 300-9, 1991 (PubMed).
Moebus, Moll, Gerharz et al.: "Establishment of new ovarian and colon carcinoma cell lines: differentiation is only possible by cytokeratin analysis." in: British journal of cancer, Vol. 69, Issue 3, pp. 422-8, 1994 (PubMed).
Moll, Zimbelmann, Goldschmidt et al.: "The human gene encoding cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas." in: Differentiation; research in biological diversity, Vol. 53, Issue 2, pp. 75-93, 1993 (PubMed).
Demirkesen, Hoede, Moll: "Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins." in: Journal of cutaneous pathology, Vol. 22, Issue 6, pp. 518-35, 1996 (PubMed).
Harnden, Eardley, Joyce et al.: "Cytokeratin 20 as an objective marker of urothelial dysplasia." in: British journal of urology, Vol. 78, Issue 6, pp. 870-5, 1997 (PubMed).
|Hosts||Mouse (58), Rabbit (51), Guinea Pig (2)|
|Reactivities||Human (102), Rat (Rattus) (32), Mouse (Murine) (30), Pig (Porcine) (3), Dog (Canine) (2), Cat (Feline) (1), Chicken (1), Cow (Bovine) (1)|
|Applications||Western Blotting (WB) (69), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (60), Immunohistochemistry (IHC) (25), ELISA (23), Immunofluorescence (IF) (21), Immunohistochemistry (Frozen Sections) (IHC (fro)) (10), Immunoprecipitation (IP) (10), Immunohistochemistry (Fixed) (IHC (fx)) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (5), Immunocytochemistry (ICC) (4), Dot Blot (DB) (1), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Biotin (3), FITC (3), HRP (2), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term (2), Internal Region (1)|