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Keratin 20 (KRT20) antibody (FITC)

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Synonyms KRT20, CD20, CK-20, CK20, K20, KRT21, Krt21, 9030623C06Rik
Human, Pig (Porcine), Rat (Rattus)
(238), (52), (43), (8), (4), (2), (1)
(118), (108), (18), (2)
Clonality (Clone)
Monoclonal ()
(12), (7), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(168), (86), (67), (58), (27), (25), (11), (11), (11), (7), (4), (4), (3), (3), (1), (1)
Pubmed 8 references available
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Quantity 0.25 mL
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Immunogen Electrophoretically purified Keratin K 20 from Human intestinal mucosa.
Clone KS20-10
Isotype IgG1
Specificity This Monoclonal antibody represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative. Polypeptide Recognized: Protein IT (keratin K20, Mr 46 000, also designated Cytokeratin 20). Tested Reactivities on Cultured Cell Lines: HT-29, LoVo, DLD-1, SW 1116, CaCo-2, RT-4.
No Cross-Reactivity Mouse (Murine)
Cross-Reactivity (Details) Species reactivity (tested):Human, Porcine (Pig) and Rat.
Purification Affinity Chromatography
Alternative Name Cytokeratin 20 (KRT20 Antibody Abstract)
Background Cytokeratin 20 is a 46 kDa intermediate filament protein whose expression is restricted primarily to gastric and intestinal epithelium, urothelium, and Merkel cells. This antibody is highly specific to cytokeratin 20 and shows no cross-reactivity with other IFPs. Cytokeratin 20 is a unique type I keratin that is expressed in adenocarcinomas of the colon stomach, pancreas and bile system. It is also expressed in mucinous ovarian tumors, transitional cell carcinomas of the urinary tract, and Merkel cell carcinomas.Synonyms: CK20, Cytokeratin 20, K20, KRT20, Keratin type I cytoskeletal 20, Keratin-20, Protein IT
Gene ID 54474
NCBI Accession NP_061883
UniProt P35900
Research Area Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix, Cancer
Application Notes FACS. Cytological Material. Immunohistochemistry on Frozen Sections. . Immunohistochemistry on Paraffin sections (Only after microwave treatment). Working Dilution: 1/10-1/20 in PBS, PH 7.4 for Immunohistochemistry. Incubation Time: 1h at RT or over night at 2-8 °C.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-Keratin 20 (KRT20) antibody (FITC) (ABIN111170) Mouse anti Cytokeratin-20 (KRT20) KS20.10
Background publications Harnden, Eardley, Joyce et al.: "Cytokeratin 20 as an objective marker of urothelial dysplasia." in: British journal of urology, Vol. 78, Issue 6, pp. 870-5, 1997 (PubMed).

Zhang, Oakley: "The distribution and origin of keratin 20-containing taste buds in rat and human." in: Differentiation; research in biological diversity, Vol. 61, Issue 2, pp. 121-7, 1997 (PubMed).

Demirkesen, Hoede, Moll: "Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins." in: Journal of cutaneous pathology, Vol. 22, Issue 6, pp. 518-35, 1996 (PubMed).

Moll, Zimbelmann, Goldschmidt et al.: "The human gene encoding cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas." in: Differentiation; research in biological diversity, Vol. 53, Issue 2, pp. 75-93, 1993 (PubMed).

Möbus, Moll, Gerharz et al.: "Establishment of new ovarian and colon carcinoma cell lines: differentiation is only possible by cytokeratin analysis." in: British journal of cancer, Vol. 69, Issue 3, pp. 422-8, 1994 (PubMed).

Moll, Moll: "Comparative cytokeratin analysis of sweat gland ducts and eccrine poromas." in: Archives of dermatological research, Vol. 283, Issue 5, pp. 300-9, 1991 (PubMed).

Moll, Schiller, Franke: "Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns." in: The Journal of cell biology, Vol. 111, Issue 2, pp. 567-80, 1990 (PubMed).

Moll, Löwe, Laufer et al.: "Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies." in: The American journal of pathology, Vol. 140, Issue 2, pp. 427-47, 1992 (PubMed).

Catalog No. ABIN111170
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