Keratin 20 (KRT20) antibody (FITC)

Antigen: Keratin 20 (KRT20)

Synonyms: K20, CD20, CK20, KRT21, MGC35423, 9030623C06Rik, Krt21, KRT20, MGC152269

Clonality: Monoclonal (KS20-10)

Host: Mouse

Reactivity: Human, Pig (Porcine), Rat (Rattus)

Conjugate: FITC

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN111170

Additional Information

Alternative name: Cytokeratin 20

UniProt: P35900

Immunogen: Electrophoretically purified keratin K 20 from human intestinal mucosa.

Cross-Reactivity: Human, Pig (Porcine), Rat (Rattus)

Isotype: IgG1

Clone: KS20-10

Description: Cytokeratin 20 is a 46 kDa intermediate filament protein whose expression is restrictedprimarily to gastric and intestinal epithelium, urothelium, and Merkel cells. This antibodyis highly specific to cytokeratin 20 and shows no cross-reactivity with other IFPs. Cytokeratin 20 is a unique type I keratin that is expressed in adenocarcinomas of the colonstomach, pancreas and bile system. It is also expressed in mucinous ovarian tumors,transitional cell carcinomas of the urinary tract, and Merkel cell carcinomas. This antibody is essentially non-reactive in squamous cell carcinomas andadenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of theovary, and small cell carcinomas. This antibody is often used in conjunction with CK7 andother antibodies to distinguishing colon carcinomas (CK20+), from ovarian, pulmonary,and breast carcinomas.

Characteristics: Synonyms: Cytokeratin 20, Keratin-20, KRT20, CK20, K20, Protein IT, Keratin type I cytoskeletal 20

Application Details

Application Notes: FACS. Cytological Material. Immunohistochemistry on Frozen Sections. . Immunohistochemistry on Paraffin sections (Only after microwave treatment). Working Dilution: 1/10-1/20 in PBS, PH 7.4 for Immunohistochemistry. Incubation Time: 1h at RT or over night at 2-8°C. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.2 mg/ml

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Protect from light. Shelf life: one year from despatch.

Research Area: Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix, Cancer

Restrictions: For Research Use only

Images

Mouse anti Cytokeratin-20 (KRT20) KS20.10

Publications

Moll, Loewe, Laufer et al.: "Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies." in: The American journal of pathology, Vol. 140, Issue 2, pp. 427-47, 1992 (PubMed).

Moll, Schiller, Franke: "Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns." in: The Journal of cell biology, Vol. 111, Issue 2, pp. 567-80, 1990 (PubMed).

Moll, Moll: "Comparative cytokeratin analysis of sweat gland ducts and eccrine poromas." in: Archives of dermatological research, Vol. 283, Issue 5, pp. 300-9, 1991 (PubMed).

Moebus, Moll, Gerharz et al.: "Establishment of new ovarian and colon carcinoma cell lines: differentiation is only possible by cytokeratin analysis." in: British journal of cancer, Vol. 69, Issue 3, pp. 422-8, 1994 (PubMed).

Moll, Zimbelmann, Goldschmidt et al.: "The human gene encoding cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas." in: Differentiation; research in biological diversity, Vol. 53, Issue 2, pp. 75-93, 1993 (PubMed).

Demirkesen, Hoede, Moll: "Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins." in: Journal of cutaneous pathology, Vol. 22, Issue 6, pp. 518-35, 1996 (PubMed).

Harnden, Eardley, Joyce et al.: "Cytokeratin 20 as an objective marker of urothelial dysplasia." in: British journal of urology, Vol. 78, Issue 6, pp. 870-5, 1997 (PubMed).