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CD163 antibody (CD163 Molecule)

Details for Product anti-CD163 Antibody No. ABIN111834, Supplier: Login to see New
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Synonyms DMBT1, CD163, CD163v2, CD163v3, M130, MM130
Rat (Rattus)
(229), (54), (35), (19), (5), (3), (2), (2), (1), (1), (1)
(207), (90)
Clonality (Clone)
Monoclonal ()
This CD163 antibody is un-conjugated
(32), (31), (30), (10), (6), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(150), (131), (68), (53), (53), (50), (43), (40), (11), (11), (8), (5), (5), (5), (4), (2), (2), (1)
Pubmed 9 references available
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Quantity 0.25 mg
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen Rat Spleen cell homogenate. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0Ag 14 mouse myeloma cell line.
Clone ED2
Isotype IgG1
Specificity This antibody recognises the Rat CD163 cell surface glycoprotein, a 175 kDa molecule also known as ED2. It does not react with Human.
Cross-Reactivity (Details) Species reactivity (tested):Rat.
Purification Affinity Chromatography on Protein A
Alternative Name CD163 (CD163 Antibody Abstract)
Background CD163 is a scavenger receptor for the haemoglobin-haptoglobin complex, and is upregulated by glucocorticoids and IL-10. The extracellular portion of the receptor is regularly shed and can be found in the circulation. An important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells. CD163 is expressed by approximately 50 % of peritoneal macrophages, a subset of splenic macrophages, and by macrophages in most other tissues. However, it is not expressed by monocytes, alveolar macrophages or microglial cells.Synonyms: Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine-rich type 1 protein M130
Gene ID 9332
NCBI Accession NP_004235
UniProt Q86VB7
Research Area Cardiovascular, Atherosclerosis, CD Antigens, Innate Immunity, Surface Receptors of Immune Cells
Application Notes Western blot. Immunoprecipitation. Flow Cytometry: Use 10 μL of 1/10-1/100 diluted CD163 antibody to label 10^6 cells in 100μL. Immunohistochemistry on Frozen Sections: 1/50-1/100Immunohistochemistry on Paraffin Sections: This product requires antigen retrieval usingpronase prior to staining of paraffin sections. IHC on paraffin-embedded material following PLP fixation(Periodate-lysine-paraformaldehyde) - refer to Whiteland et al (Ref.5. )Histology Positive Control Tissue: liver.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Schwartzkopff, Schlereth, Berger et al.: "NK cell depletion delays corneal allograft rejection in baby rats." in: Molecular vision, Vol. 16, pp. 1928-35, 2010 (PubMed).

Bedi, Kovacevic, Fox et al.: "Effect of early and delayed mechanical loading on tendon-to-bone healing after anterior cruciate ligament reconstruction." in: The Journal of bone and joint surgery. American volume, Vol. 92, Issue 14, pp. 2387-401, 2010 (PubMed).

Fujita, Shimizu, Masuda et al.: "Statin attenuates experimental anti-glomerular basement membrane glomerulonephritis together with the augmentation of alternatively activated macrophages." in: The American journal of pathology, Vol. 177, Issue 3, pp. 1143-54, 2010 (PubMed).

Deng, Wang, Elm et al.: "Chronic alcohol consumption accelerates fibrosis in response to cerulein-induced pancreatitis in rats." in: The American journal of pathology, Vol. 166, Issue 1, pp. 93-106, 2005 (PubMed).

Dorval-Coiffec, Delcros, Hakovirta et al.: "Identification of the leukemia inhibitory factor cell targets within the rat testis." in: Biology of reproduction, Vol. 72, Issue 3, pp. 602-11, 2005 (PubMed).

Banerjee, Figueiredo, Easty et al.: "Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats." in: The British journal of ophthalmology, Vol. 87, Issue 12, pp. 1515-22, 2003 (PubMed).

Dijkstra, Damoiseaux: "Macrophage heterogeneity established by immunocytochemistry." in: Progress in histochemistry and cytochemistry, Vol. 27, Issue 2, pp. 1-65, 1993 (PubMed).

Whiteland, Nicholls, Shimeld et al.: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995 (PubMed).

Dijkstra, Döpp, Joling et al.: "The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3." in: Immunology, Vol. 54, Issue 3, pp. 589-99, 1985 (PubMed).

Catalog No. ABIN111834
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