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CD163 Molecule (CD163) antibody

Details for Product No. ABIN111834
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Antigen
Synonyms DMBT1, CD163, CD163v2, CD163v3, M130, MM130
Reactivity
Rat (Rattus)
(142), (34), (24), (18), (17), (17), (5), (1)
Host
Mouse
(112), (53), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(22), (15), (14), (5), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(85), (65), (62), (43), (29), (20), (18), (14), (13), (8), (6), (4)
Pubmed 9 references available
Quantity 0.25 mg
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Catalog No. ABIN111834
566.50 $
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Immunogen Rat Spleen cell homogenate. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0Ag 14 mouse myeloma cell line.
Clone ED2
Isotype IgG1
Specificity This antibody recognises the Rat CD163 cell surface glycoprotein, a 175kD molecule also known as ED2. It does not react with Human. Species Reactivity: Tested: Rat.
Purification Affinity Chromatography on Protein A
Alternative Name CD163
Background CD163 is a scavenger receptor for the haemoglobin-haptoglobin complex, and is upregulated by glucocorticoids and IL-10. The extracellular portion of the receptor is regularly shed and can be found in the circulation. An important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells. CD163 is expressed by approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and by macrophages in most other tissues. However, it is not expressed by monocytes, alveolar macrophages or microglial cells.
Alternate names: Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine- rich type 1 protein M130
Gene ID 9332
NCBI Accession NP_004235
UniProt Q86VB7
Research Area Cardiovascular, Atherosclerosis, CD Antigens, Innate Immunity, Surface Receptors of Immune Cells
Application Notes Western blot. Immunoprecipitation. Flow Cytometry: Use 10 µl of 1/10-1/100 diluted CD163 antibody to label 10e6 cells in 100µl. Immunohistochemistry on Frozen Sections: 1/50-1/100Immunohistochemistry on Paraffin Sections: This product requires antigen retrieval usingpronase prior to staining of paraffin sections. IHC on paraffin-embedded material following PLP fixation(Periodate-lysine-paraformaldehyde) - refer to Whiteland et al (Ref.5. )Histology Positive Control Tissue: liver. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS, pH 7.4 with 0.09% Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Dijkstra, Döpp, Joling et al.: "The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3." in: Immunology, Vol. 54, Issue 3, pp. 589-99, 1985 (PubMed).

Whiteland, Nicholls, Shimeld et al.: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995 (PubMed).

Banerjee, Figueiredo, Easty et al.: "Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats." in: The British journal of ophthalmology, Vol. 87, Issue 12, pp. 1515-22, 2003 (PubMed).

Dorval-Coiffec, Delcros, Hakovirta et al.: "Identification of the leukemia inhibitory factor cell targets within the rat testis." in: Biology of reproduction, Vol. 72, Issue 3, pp. 602-11, 2005 (PubMed).

Deng, Wang, Elm et al.: "Chronic alcohol consumption accelerates fibrosis in response to cerulein-induced pancreatitis in rats." in: The American journal of pathology, Vol. 166, Issue 1, pp. 93-106, 2005 (PubMed).

Fujita, Shimizu, Masuda et al.: "Statin attenuates experimental anti-glomerular basement membrane glomerulonephritis together with the augmentation of alternatively activated macrophages." in: The American journal of pathology, Vol. 177, Issue 3, pp. 1143-54, 2010 (PubMed).

Bedi, Kovacevic, Fox et al.: "Effect of early and delayed mechanical loading on tendon-to-bone healing after anterior cruciate ligament reconstruction." in: The Journal of bone and joint surgery. American volume, Vol. 92, Issue 14, pp. 2387-401, 2010 (PubMed).

Schwartzkopff, Schlereth, Berger et al.: "NK cell depletion delays corneal allograft rejection in baby rats." in: Molecular vision, Vol. 16, pp. 1928-35, 2010 (PubMed).

General Dijkstra, Damoiseaux: "Macrophage heterogeneity established by immunocytochemistry." in: Progress in histochemistry and cytochemistry, Vol. 27, Issue 2, pp. 1-65, 1993 (PubMed).

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