Glial Fibrillary Acidic Protein (GFAP) antibody

Details for Product No. ABIN111958
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Antigen
Synonyms GFAP, AI836096, cb345, etID36982.3, gfapl, wu:fb34h11, wu:fk42c12, xx:af506734, zgc:110485
Reactivity
Cat (Feline), Human, Pig (Porcine)
(434), (149), (146), (81), (63), (17), (15), (12), (11), (8), (6), (5), (4), (2), (2), (1), (1), (1), (1), (1)
Host
Mouse
(273), (237), (24), (10), (3), (3), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(21), (16), (15), (8), (8), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(388), (213), (177), (173), (116), (109), (73), (40), (30), (10), (5), (5), (5), (4), (3), (2), (1), (1), (1)
Pubmed 3 references available
Quantity 0.1 mg
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Catalog No. ABIN111958
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Immunogen Pellet of Porcine brain cold-stable proteins after depolymerization of microtubules.
Clone GF-01
Isotype IgG2b
Specificity The antibody reacts with GFAP molecules. GFAP is the principal marker of astroglial cells in the central nervous system, it is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves. The GFAP protein runs on gels at ~55 kDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene.
Purification Protein-A Affinity Chromatography
Purity > 95 % (by SDS-PAGE).
Alternative Name GFAP
Background Glial Fibrillary Acidic Protein (GFAP) was discovered by Bignami et al. (1972) as a major fibrous protein of multiple sclerosis plaques. It was subsequently found to be a member of the 10 nm or intermediate filament protein family, specifically the intermediate filament protein family Class III, which also includes peripherin, desmin and vimentin. GFAP is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. It is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. Although its function is not fully understood, GFAP protein is probably involved in controlling the shape and movement of astrocytes. The protein probably also plays a significant role in the interactions of astrocytes with other cells, which are required for the formation and maintenance of the insulating layer (myelin) that covers nerve cells. Additionally, GFAP protein may assist in maintaining the protective barrier that allows only certain substances to pass between blood vessels and the brain (blood-brain barrier). In adults, GFAP levels increase as a result of the proliferation of astrocytes that occurs in a response to a variety of physical, chemical and etiological insults, including Alzheimers disease, epilepsy and multiple sclerosis. Antibodies to GFAP are therefore very useful as markers of astrocytic cells and neural stem cells and for distinguishing of neoplasms of astrocytic origin from other neoplasms in the central nervous system. Finally, Alexander's disease was recently shown to be caused by point mutations in protein coding region of the GFAP gene (Brenner et al., 2001). All forms of Alexander disease are characterized by the presence of Rosenthal fibers, which are GFAP containing cytoplasmic inclusions found in astrocytes.
Alternate names: Glial Fibrillary Acidic Protein
Gene ID 2670
NCBI Accession NP_001229305
UniProt P14136
Research Area Stem Cells, Cytoskeleton, Glia marker, Cell/Tissue Markers, Neurology
Application Notes Western Blotting. Immunoprecipitation. Immunocytochemistry: 5-10 µg/ml. Immunohistochemistry on Paraffin Sections: 10 µg/ml. The antibody strongly stains astrocytes in Human brain tissue sections but it is essentiallynegative on Mouse and Rat tissues. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), pH~7.4 with 15 mM Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C longer.
Expiry Date 12 months
Background publications Lukás, Dráber, Bucek et al.: "Expression of vimentin and glial fibrillary acidic protein in human developing spinal cord." in: The Histochemical journal, Vol. 21, Issue 12, pp. 693-701, 1990 (PubMed).

Bignami, Eng, Dahl et al.: "Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence." in: Brain research, Vol. 43, Issue 2, pp. 429-35, 1972 (PubMed).

Brenner, Johnson, Boespflug-Tanguy et al.: "Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease." in: Nature genetics, Vol. 27, Issue 1, pp. 117-20, 2001 (PubMed).

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