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ACTC1 antibody

ACTC1 Reactivity: Human, Rabbit, Chicken, Cow WB, IHC (p), IHC (fro), EIA Host: Mouse Monoclonal AC1-20-4-2 unconjugated
Catalog No. ABIN112213
  • Target See all ACTC1 Antibodies
    ACTC1 (Actin, Alpha, Cardiac Muscle 1 (ACTC1))
    Reactivity
    • 36
    • 26
    • 24
    • 9
    • 4
    • 4
    • 2
    • 2
    • 2
    Human, Rabbit, Chicken, Cow
    Host
    • 22
    • 16
    Mouse
    Clonality
    • 20
    • 18
    Monoclonal
    Conjugate
    • 24
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This ACTC1 antibody is un-conjugated
    Application
    • 23
    • 11
    • 9
    • 7
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
    Specificity
    This antibody is specific for Fetal (Cardiac) isoform of Actin. Clone Ac1 represents an excellent marker for cardiac tissue. It discriminates fetal (cardiac) alpha-Actin from all other Actin isoforms. Mab Ac1-20.4.2 shows no cross reaction with other Actin isoforms present in skeletal and smooth muscle, provided that stringent conditions have been applied (see Special Treatment).
    Cross-Reactivity (Details)
    Species reactivity (tested):Human, Bovine, Rabbit and Chicken.
    Purification
    Affinity Chromatographyon Protein G.
    Immunogen
    Synthetic NH2 terminus decapeptide of cardiac isoform of Actin.
    Clone
    AC1-20-4-2
    Isotype
    IgG1
    Top Product
    Discover our top product ACTC1 Primary Antibody
  • Application Notes
    ELISA: 1/500. Western Blot: 1/1000 (using the ECL-enhanced procedure). Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded Tissue: 1/10 (After microwave treatment). Incubation time: 1 h at RT. Special Treatment: It is necessary to include 0.5 M NaCl in all washing buffers forapplication on Frozen tissue and 1 M NaCl for Western blotting and ELISA to enhancespecifity. This is not necessary for Paraffin sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore in 1.0 mL distilled water.
    Buffer
    Final Solution contains PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Skwarek-Maruszewska, Hotulainen, Mattila, Lappalainen: "Contractility-dependent actin dynamics in cardiomyocyte sarcomeres." in: Journal of cell science, Vol. 122, Issue Pt 12, pp. 2119-26, (2009) (PubMed).

    Itzhaki-Alfia, Leor, Raanani, Sternik, Spiegelstein, Netser, Holbova, Pevsner-Fischer, Lavee, Barbash: "Patient characteristics and cell source determine the number of isolated human cardiac progenitor cells." in: Circulation, Vol. 120, Issue 25, pp. 2559-66, (2009) (PubMed).

    Lechler, Wu, Bernhardt, Campean, Gastiger, Hackenbeck, Klanke, Weidemann, Warnecke, Amann, Engehausen, Willam, Eckardt, Rödel, Wiesener: "The tumor gene survivin is highly expressed in adult renal tubular cells: implications for a pathophysiological role in the kidney." in: The American journal of pathology, Vol. 171, Issue 5, pp. 1483-98, (2007) (PubMed).

  • Target
    ACTC1 (Actin, Alpha, Cardiac Muscle 1 (ACTC1))
    Abstract
    ACTC1 Products
    Synonyms
    acta1 antibody, actc antibody, fa27h01 antibody, fb83f06 antibody, wu:fa27h01 antibody, wu:fb83f06 antibody, ACTC antibody, ASD5 antibody, CMD1R antibody, CMH11 antibody, LVNC4 antibody, Actc-1 antibody, actcl antibody, actc1l antibody, cmd1r antibody, ACTC1 antibody, actin, alpha, cardiac muscle 1 L homeolog antibody, actin, alpha, cardiac muscle 1b antibody, actin, alpha, cardiac muscle 1 antibody, actin, alpha, cardiac muscle 1a antibody, actc1.L antibody, actc1b antibody, ACTC1 antibody, Actc1 antibody, actc1a antibody, actc1 antibody
    Background
    Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. ACTB/ACTC are nonmuscle cytoskeletal actins and major constituents of the contractile apparatus. Defects in ACTB are a cause of juvenile-onset dystonia. Defects in ACTC have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). Fetal Actin can be localized in regenerating skeletal muscle after injury (in satellite cells) and in veins of the umbelical cord.
    Gene ID
    70
    NCBI Accession
    NP_005150
    UniProt
    P68032
    Pathways
    Myometrial Relaxation and Contraction
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