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Coagulation Factor VIII (F8) antibody

Details for Product No. ABIN112268
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Antigen
Synonyms fb61d02, wu:fb61d02, Cf8, Cf-8, FVIII, AHF, F8B, F8C, HEMA, DXS1253E
Reactivity
Human
(47), (3), (2), (1), (1)
Host
Mouse
(35), (16), (1), (1), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Western Blotting (WB)
(41), (38), (10), (8), (6), (5), (3), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
Quantity 0.5 mg
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Catalog No. ABIN112268
742.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Human factor VIII antigen
Clone MH104
Isotype IgG2a
Specificity MH104 is specific for human factor VIIIc, a 300 kD protein present in plasma in a complex with von Willebrandt factor. Involved in the clotting cascade (activated by thrombin) by forming a complex with factor IXa, calcium, and phospholipids. Elevated levels of factor VIII have been associated with acute and chronic liver diseases, vascular disorders, diabetes and with acutephase reactions.
Purification Protein A affinity chromatography
Background Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. It is an extracellular factor. Defects in F8 are the cause of hemophilia A (HEMA). HEMA is a common recessive X linked coagulation disorder. The frequency of hemophilia A is 1-2 in 10,000 male births in all ethnic groups. About 50% of patients have severe hemophilia A with F8C activity less than 1% of normal, they have frequent spontaneous bleeding into joints, muscles and internal organs. Moderately severe hemophilia A occurs in about 10% of patients, F8C activity is 2-5% of normal, and there is bleeding after minor trauma. Mild hemophilia A, which occurs in 30-40% of patients, is associated with F8C activity of 5-30% and bleeding occurs only after significant trauma or surgery. Of particular interest for the understanding of the function of F8C is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8C in their plasma (at least 30% of normal), but the protein is nonfunctional, i.e., the F8C activity is much less than the plasma protein level. CRM reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein.
Alternate names: AHF, Antihemophilic factor, F8C, Procoagulant component
Gene ID 2157
NCBI Accession NP_000123.1
UniProt P00451
Application Notes ELISA: 1: 5,000, detection and quantitation of human factor VIII. Immunoblotting. Immunohistochemistry on frozen sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore in 1 mL dist. water
Buffer PBS, pH 7.4 containing 0.09 % Sodium azide, 0.5% BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Prior to and following reconstitution store the antibody undiluted at 2-8°C. DO NOT FREEZE!
Expiry Date 12 months
Background publications Stel, van der Kwast, Veerman: "Detection of factor VIII/coagulant antigen in human liver tissue." in: Nature, Vol. 303, Issue 5917, pp. 530-2, 1983 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN112268
742.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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