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Details for Product No. ABIN114256

CD90 (THY1) antibody (PE)

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Antigen
Synonyms CD90, CD7
Reactivity
»Alternatives Mouse (Murine)
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
Application
»Alternatives Flow Cytometry (FACS)
Pubmed 10 references available
Catalog no. ABIN114256
Quantity 0.3 mg
Price
599.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen CBA/J. Donor: AKR/J Spleen. Fusion Partner: Spleen from immunized recipient fused with myeloma P3-NSI-1-Ag4-1.
Clone 5a-8
Isotype IgG2b
Specificity This monoclonal antibody reacts with all T lymphocytes from mouse strains expressing the Thy 1.2 phenotype (e.g. C57BL/6, C3H/He, DBA/2, CBA/J, BALB/c), but does not react with lymphocytes expressing the Thy 1.1 phenotype [e.g. AKR/J, B6.PL(74NS)].
Purification Protein G Chromatography
Alternative Name CD90
Background CD90 / Thy1 antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.
Alternate names: CDw90, THY1, Thy-1, Thy-1 membrane glycoprotein
Gene ID 21838
NCBI Accession NP_033408.1
UniProt P01831
Research Area CD Antigens, Surface Receptors of Immune Cells, Experimental Models
Application Notes Flow Cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0. 5 µg* of this Ab. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes areprotected from light, since most fluorochromes are light sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 5 µg/10e6 cellsIsotypic Control: PE-Mouse IgG2b,kCell Source Percentage of cells stained above control: Thymus: 98. 4%Spleen: 28. 8%Results - Strain Distribution: Cell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 5 µg/10e6 cellsStrains Tested: C57BL/6, C3H/He, CBA/J, BALB/c, ATL, AKR/JPositive: C57BL/6, C3H/He, CBA/J, BALB/c, ATLNegative: AKR/J
Restrictions For Research Use only
Format Liquid
Concentration 0.1 mg/mL
Buffer PBS, 0.02% Sodium azide and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml
Preservative Sodium azide
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8°C. DO NOT FREEZE!
Expiry Date 12 months
Background publications Miyajima, Hirano, Hirose et al.: "Suppression by IL-2 of IgE production by B cells stimulated by IL-4." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 2, pp. 457-62, 1991 (PubMed).

Krieg, Gourley, Steinberg: "Association of murine lupus and thymic full-length endogenous retroviral expression maps to a bone marrow stem cell." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 9, pp. 3002-5, 1991 (PubMed).

Kruger, Riley: "The age-dependent loss of bone marrow B cell precursors in autoimmune NZ mice results from decreased mitotic activity, but not from inherent stromal cell defects." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 1, pp. 103-10, 1990 (PubMed).

Fine, Silverstone, Gasiewicz: "Impairment of prothymocyte activity by 2,3,7,8-tetrachlorodibenzo-p-dioxin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 4, pp. 1169-76, 1990 (PubMed).

Siegelman, Cheng, Weissman et al.: "The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding." in: Cell, Vol. 61, Issue 4, pp. 611-22, 1990 (PubMed).

Jalkanen, Bargatze, de los Toyos et al.: "Lymphocyte recognition of high endothelium: antibodies to distinct epitopes of an 85-95-kD glycoprotein antigen differentially inhibit lymphocyte binding to lymph node, mucosal, or synovial endothelial cells." in: The Journal of cell biology, Vol. 105, Issue 2, pp. 983-90, 1987 (PubMed).

Lewinsohn, Bargatze, Butcher: "Leukocyte-endothelial cell recognition: evidence of a common molecular mechanism shared by neutrophils, lymphocytes, and other leukocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 12, pp. 4313-21, 1987 (PubMed).

Fink, Gallatin, Reichert et al.: "Homing receptor-bearing thymocytes, an immunocompetent cortical subpopulation." in: Nature, Vol. 313, Issue 5999, pp. 233-5, 1985 (PubMed).

Reichert, Gallatin, Butcher et al.: "A homing receptor-bearing cortical thymocyte subset: implications for thymus cell migration and the nature of cortisone-resistant thymocytes." in: Cell, Vol. 38, Issue 1, pp. 89-99, 1984 (PubMed).

Gallatin, Weissman, Butcher: "A cell-surface molecule involved in organ-specific homing of lymphocytes." in: Nature, Vol. 304, Issue 5921, pp. 30-4, 1983 (PubMed).

Alternatives for antigen "CD90 (THY1)", type "Antibodies"
Hosts (218), (197), (59), (4)
Reactivities (302), (137), (119), (32), (27), (19), (18), (15), (8), (1), (1), (1)
Applications (366), (97), (78), (54), (48), (37), (36), (34), (29), (27), (20), (10), (8), (7), (2), (1), (1), (1)
Conjugates (67), (63), (47), (22), (9), (7), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (7), (7), (3), (2), (1), (1)
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