T Cell Receptor (TCR) alpha/beta (alpha chain) antibody (Biotin)

Details for Product No. ABIN114324
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Antigen
Reactivity
Mouse (Murine)
(29), (16), (13), (6)
Host
Hamster
(48), (12)
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
(14), (13), (8), (2), (1)
Application
Flow Cytometry (FACS)
(52), (23), (14), (5), (5), (2), (1), (1), (1)
Pubmed 5 references available
Quantity 0.3 mg
Shipping to United States (Change)
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Catalog No. ABIN114324
368.50 $
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Immunogen Affinity Purified D0-11.10 TCR Donor: Armenian Hamster Fusion Partner: Murine myeloma variant P3X63Ag.653
Clone H57-597
Isotype IgG
Specificity This mAb reacts with the surface of all ab TCR bearing cells and does not react with receptors on gd TCR positive T cells. When used in an immobilized form, this antibody was able to activate all ab TCR bearing T cell hybridomas tested to produce IL-2. Use of this antibody in conjunction with an anti-CD3e mAb allows for accurate measurements of the mutually exclusive sub-populations of ab TCR and gd TCR bearing T cells.
Purification Protein G Chromatography
Alternative Name T Cell Receptor (TCR) alpha/beta
Background Alternate names: T-Cell Receptor alpha, T-Cell Receptor alpha beta, T-Cell Receptor beta, TCRA, TCRB
Research Area Immunology, Receptors, Signaling
Application Notes Flow cytometry. Immunoprecipitation. This clone has also been reported to work with frozen sections and Western Blotting. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M, cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 1. 0 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (Streptavidin-FITC) at a 1: 500 dilution. 9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protectedfrom light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution by Flow Cytometry Analysis: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testsAntibody Concentration Used: 1. 0 µg/10e6 cellsIsotypic Control: Biotin Hamster IgGCell Source Percentage of cells stained above control: Thymus: 62. 6% see FIGURE 1Splenic T Cells: 76. 2%Strain Distribution by Flow Cytometry Analysis: Cell Concentration: 1x10e6 cells per testsAntibody Concentration Used: 1. 0 µg/106 cellsStrains Tested: BALB/c, C57BL/6, CBA/J, C3H/He, AKRPositive: BALB/c, C57BL/6, CBA/J, C3H/He, AKRNegative: none
Restrictions For Research Use only
Format Liquid
Concentration 0.1 mg/mL
Buffer PBS,0.02% Sodium azide and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-T Cell Receptor (TCR) alpha/beta (alpha chain) antibody (Biotin) Figure 1
Background publications Kubo, Born, Kappler et al.: "Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 8, pp. 2736-42, 1989 (PubMed).

Goodman, Lefrancois: "Intraepithelial lymphocytes. Anatomical site, not T cell receptor form, dictates phenotype and function." in: The Journal of experimental medicine, Vol. 170, Issue 5, pp. 1569-81, 1989 (PubMed).

General Palathumpat, Holm, Dejbakhsh-Jones et al.: "Treatment of BCL1 leukemia by transplantation of low density fractions of allogeneic bone marrow and spleen cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 10, pp. 3319-26, 1992 (PubMed).

Skarstein, Holmdahl, Johannessen et al.: "Oligoclonality of T cells in salivary glands of autoimmune MRL/lpr mice." in: Immunology, Vol. 81, Issue 4, pp. 497-501, 1994 (PubMed).

Paliwal, Ptak, Sperl et al.: "Recombinant soluble alphabeta T cell receptors protect T cells from immune suppression: requirement for aggregated multimeric, disulfide-linked alphabeta heterodimers." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 4, pp. 1718-27, 1997 (PubMed).

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