Colony Stimulating Factor 3 Receptor (Granulocyte) (CSF3R) antibody

Antigen: Colony Stimulating Factor 3 Receptor (Granulocyte) (CSF3R)

Synonyms: CD114, GCSFR, Cd114, Csfgr, G-CSFR, MGC141472, MGC141473, CSF3R, csf3r, MGC80392, gcsfr

Clonality: Monoclonal (S-425)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Catalog no.: ABIN114568

Additional Information

Alternative name: CD114 / CSF3R

UniProt: Q99062

Immunogen: Purified recombinant MBP-GCSFR fusion protein

Cross-Reactivity: Human

Isotype: IgG2a

Clone: S-425

Description: The protein encoded by the GCSF Receptor gene is the receptor for colony stimulatingfactor 3, a cytokine that controls the production, differentiation, and function ofgranulocytes. The encoded protein, which is a member of the family of cytokine receptors,may also function in some cell surface adhesion or recognition processes. Four transcriptvariants encoding four different isoforms have been found for this gene, of which three aremembrane-bound and the one secreted and soluble. Mutations in this gene are a cause ofKostmann syndrome, also known as severe congenital neutropenia.

Characteristics: Synonyms: G-CSF-R, GCSFR, GCSF Receptor, G-CSF Receptor, Granulocyte colony-stimulating factorreceptor

Specificity: Reactive with G-CSFR and does not cross-react with MBP or BSA (bovine serum albumin). Species: Human. Others not tested.

Application Details

Application Notes: ELISA. Immunoblotting: Recognizes denatured G-CSFR following SDS-PAGE and blotting of 200 ugof cell lysate or following immunoprecipitation with polyclonal antibody followed byimmunoblotting. Western Blot: At an antibody concentration of 1-10 ug/mL will allow visualization of 250ng/lane of human G-CSFR-MBP. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Buffer: 0.01M PBS, pH 7.2Reconstitution: Restore with double distillated water is recommended to adjust the finalconcentration to 1.00 mg/ml

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Baars, De Ruijter, Smedts et al.: "The applicability of a keratin 7 monoclonal antibody in routinely Papanicolaou-stained cytologic specimens for the differential diagnosis of carcinomas." in: American journal of clinical pathology, Vol. 101, Issue 3, pp. 257-61, 1994 (PubMed).

Hernandez, Frierson, Moskaluk et al.: "CK20 and CK7 protein expression in colorectal cancer: demonstration of the utility of a population-based tissue microarray." in: Human pathology, Vol. 36, Issue 3, pp. 275-81, 2005 (PubMed).