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Rabbit anti-Human IgE Antibody

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Antigen
Reactivity
Human
369
102
35
28
5
3
2
2
2
2
1
1
1
Host
Rabbit
221
206
58
37
5
2
1
1
Clonality
Polyclonal
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Immunogen IgE isolated from the serum of a patient with IgE-myeloma.
Purification Purified
Background IgE is typically the least abundant isotype - blood serum IgE levels in a normal ("non-atopic") individual are ~150 ng/mL, compared to 10 mg/mL for the IgGs - it is capable of triggering the most powerful immune reactions. Most of our knowledge of IgE has come from research into the mechanism of a form of allergy known as type 1 hypersensitivity. There is much speculation into what physiological benefits IgE contributes, and so far, circumstantial evidence in animal models and statistical population trends have hinted that IgE may be beneficial in fighting gut parasites such as Schistosoma mansoni, but this has not been conclusively proven in humans. IgE may play an important role in the immune system's recognition of cancer, in which the stimulation of a strong cytotoxic response against cells displaying only small amounts of early cancer markers would be beneficial. IgE may be an important target in treatments for allergy and asthma.Synonyms: Immunoglobulin E human
Research Area Immunology, Secondary Antibodies
Application Notes Immunohistochemistry on Formalin-Fixed Praffin Embedded Sections: Use at a dilution of1/50-1/500 in an ABC method (30 minutes at room temperature) Recommended Positive Control: Tonsil.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
Background publications Kuida, Haydar, Kuan et al.: "Reduced apoptosis and cytochrome c-mediated caspase activation in mice lacking caspase 9." in: Cell, Vol. 94, Issue 3, pp. 325-37, 1998 (PubMed).

Hu, Benedict, Wu et al.: "Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 8, pp. 4386-91, 1998 (PubMed).

Skulachev: "Cytochrome c in the apoptotic and antioxidant cascades." in: FEBS letters, Vol. 423, Issue 3, pp. 275-80, 1998 (PubMed).