Aldolase A / ALDOA antibody

Details for Product No. ABIN116608
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Human, Rabbit
(5), (2), (1), (1)
(3), (1), (1)
Enzyme Immunoassay (EIA), Western Blotting (WB)
(5), (4), (4), (4), (2), (1)
Pubmed 1 reference available
Quantity 2 mL
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Catalog No. ABIN116608
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Immunogen Aldolase [Rabbit Muscle].
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Aldolase [Rabbit Muscle]. This antibody will detect human Aldolase. Cross reactivity against Aldolase from other tissues and species may also occur. It has been reported that this antibody can detect human Aldolase on immunoblot showing a 41 kDa band in lysates from MCF7, NMB231 and HBL100 cell lines.
Purification Prepared from monospecific antiserum by a delipidation and defibrination.
Background Synonyms: ALDA, Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, NY-LU-1
Gene ID 100009055
NCBI Accession NP_001075707
UniProt P00883
Research Area Enzymes
Application Notes Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostImmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 m g of Aldolase[Rabbit Muscle] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Goat IgG [H&L] (Rabbit) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 3,000 to1: 12,000 of the reconstitution concentration is suggested for this product. Useapproximately 5 μL of antibody to immunoprecipitate 50 μL of protein lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Reconstitution Restore with 2.0 mL of deionized water (or equivalent).
Concentration 90.0 mg/mL (by Refractometry)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Storage -20 °C
Storage Comment Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.
Background publications Zaman, Ryu, Hall et al.: "Protection from oxidative stress-induced apoptosis in cortical neuronal cultures by iron chelators is associated with enhanced DNA binding of hypoxia-inducible factor-1 and ATF-1/CREB and increased expression of glycolytic enzymes, p21(waf1/cip1) ..." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 22, pp. 9821-30, 1999 (PubMed).

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