P-Selectin antibody

Catalog No. ABIN1177016

Product Details anti-P-Selectin Antibody

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This P-Selectin antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Blocking Reagent (BR)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: P-selectin-IgG1 Fusion

Clone: RB40-34

Isotype: IgG1 lambda

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-P-Selectin antibody (ABIN1177016)

Hirata, Furie, Furie: "P-, E-, and L-selectin mediate migration of activated CD8+ T lymphocytes into inflamed skin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 8, pp. 4307-13, (2002) (PubMed).

Katakai, Mori, Masuda, Shimizu: "Selective accumulation of type 1 effector cells expressing P-selectin ligand and/or alpha(4)beta(7)-integrin at the lesions of autoimmune gastritis." in: International immunology, Vol. 14, Issue 2, pp. 167-75, (2002) (PubMed).

Tietz, Allemand, Borges, von Laer, Hallmann, Vestweber, Hamann: "CD4+ T cells migrate into inflamed skin only if they express ligands for E- and P-selectin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 2, pp. 963-70, (1998) (PubMed).

Austrup, Vestweber, Borges, Löhning, Bräuer, Herz, Renz, Hallmann, Scheffold, Radbruch, Hamann: "P- and E-selectin mediate recruitment of T-helper-1 but not T-helper-2 cells into inflammed tissues." in: Nature, Vol. 385, Issue 6611, pp. 81-3, (1997) (PubMed).

Yang, Galipeau, Kozak, Furie, Furie: "Mouse P-selectin glycoprotein ligand-1: molecular cloning, chromosomal localization, and expression of a functional P-selectin receptor." in: Blood, Vol. 87, Issue 10, pp. 4176-86, (1996) (PubMed).

Bosse, Vestweber: "Only simultaneous blocking of the L- and P-selectin completely inhibits neutrophil migration into mouse peritoneum." in: European journal of immunology, Vol. 24, Issue 12, pp. 3019-24, (1995) (PubMed).

Ley, Bullard, Arbonés, Bosse, Vestweber, Tedder, Beaudet: "Sequential contribution of L- and P-selectin to leukocyte rolling in vivo." in: The Journal of experimental medicine, Vol. 181, Issue 2, pp. 669-75, (1995) (PubMed).

Target Details for P-Selectin

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Alternative Name: CD62P (P-selectin) (SELP Products)

Synonyms: PSELECT antibody, CD62 antibody, CD62P antibody, GMP140 antibody, GRMP antibody, LECAM3 antibody, PADGEM antibody, PSEL antibody, GMP-140 antibody, Grmp antibody, selectin P antibody, selectin, platelet antibody, Selp antibody, SELP antibody

Background: The RB40.34 antibody reacts with mouse P-selectin (CD62P), a 140 kDa protein which is expressed on activated platelets, activated endothelial cells, and megakaryocytes. P-selectin mediates the adhesion of neutrophils and monocytes to activated platelets and endothelial cells, mediates leukocyte rolling, and is involved in the migration of leukocytes into inflamed tissues. CD24 and CD162 (PSGL-1) are ligands of CD62P. mAb RB40.34 can block mouse P-selectin binding to its ligands in vitro and in vivo.
Synonyms: P-selectin