ElF4E (eIF-4E, CBP, EIF4E) (pSer209) antibody (Alexa Fluor 647)

Catalog No. ABIN1177053

Product Details

Target: ElF4E (eIF-4E, CBP, EIF4E)

Binding Specificity: pSer209

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Alexa Fluor 647

Application: Intracellular Staining (ICS)

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human eIF4E Peptide

Clone: J77-925

Isotype: IgG1 kappa

Application Details

Application Notes: Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation. Any of the three BD™ Phosflow permeabilization buffers may be used.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References

Culjkovic, Topisirovic, Borden: "Controlling gene expression through RNA regulons: the role of the eukaryotic translation initiation factor eIF4E." in: Cell cycle (Georgetown, Tex.), Vol. 6, Issue 1, pp. 65-9, (2007) (PubMed).

Wendel, Silva, Malina, Mills, Zhu, Ueda, Watanabe-Fukunaga, Fukunaga, Teruya-Feldstein, Pelletier, Lowe: "Dissecting eIF4E action in tumorigenesis." in: Genes & development, Vol. 21, Issue 24, pp. 3232-7, (2007) (PubMed).

Grolleau, Kaplan, Hanash, Beretta, Richardson: "Impaired translational response and increased protein kinase PKR expression in T cells from lupus patients." in: The Journal of clinical investigation, Vol. 106, Issue 12, pp. 1561-8, (2001) (PubMed).

Waskiewicz, Flynn, Proud, Cooper: "Mitogen-activated protein kinases activate the serine/threonine kinases Mnk1 and Mnk2." in: The EMBO journal, Vol. 16, Issue 8, pp. 1909-20, (1997) (PubMed).

Target Details

Target: ElF4E (eIF-4E, CBP, EIF4E)

Background: The eukaryotic translation Initiation Factor 4E (eIF4E) is a 25-kDa phosphoprotein that specifically binds to the 7-methylguanosine-containing cap of mRNA. eIF4E is the rate-limiting component for the initiation of cap-dependent translation by the eIF4F translation initiation complex, which is composed of eIF4E, eIF4G, and eIF4A. This complex promotes the unwinding of secondary structure at the 5' untranslated region of mRNA that is necessary to expose and locate the AUG initiation codon. Other functions of eIF4E have been identified, such as promoting the export of mRNAs that are involved in cell cycle progression from the nucleus and differentially regulating the translation of certain mRNAs in the cytoplasm. Three mechanisms for eIF4E regulation have been identified: Mnk1-mediated phosphorylation on serine 209 (S209) is required for eIF4E binding to the cap structure, over-expression of phosphorylated eIF4E can lead to increased cell proliferation, suppression of apoptosis, and a transformed phenotype, and interactions with nonphosphorylated eIF4E-binding proteins inhibit the formation of the eIF4F complex. The J77-925 monoclonal antibody recognizes the phosphorylated S209 (pS209) of eIF4E.
Synonyms: eIF-4E, CBP, EIF4E1, EIF4EL1, EIF4F, mRNA cap-binding protein