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Zinc Finger Protein 148 (ZNF148) antibody

Details for Product No. ABIN117932, Supplier: Log in to see
  • BERF-1
  • BFCOL1
  • ZBP-89
  • ZFP148
  • pHZ-52
  • 2210405J08Rik
  • AI480666
  • AW045217
  • Znf148
  • Zbp-89
  • fb06h05
  • wu:fb06h05
  • zfp149
  • zgc:165342
  • SLC12A8
  • zinc finger protein 148
  • ZNF148
  • Zfp148
  • znf148
Enzyme Immunoassay (EIA), Western Blotting (WB)
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Immunogen Purified full length ZBP-89 recombinant protein expressed in E.coli
Sequence 1 mniddklegl flkcggidem qssrtmvvmg gvsgqstvsg elqdsvlqdr smphqeilaa 61 devlqesemr qqdmishdel mvheetvknd eeqmetherl pqglqyalnv pisvkqeitf 121 tdvseqlmrd kkqirepvdl qkkkkrkqrs pakiltined gslglktpks hvcehcnaaf 181 rtnyhlqrhv fihtgekpfq csqcdmrfiq kyllqrheki htgekpfrcd ecgmrfiqky 241 hmerhkrths gekpyqceyc lqyfsrtdrv lkhkrmchen hdkklntcai kgglltseed 301 sgfstspkdn slpkkkrqkt ekkssgmdke saldksdlkk dkndylpvys sstkvkdeym 361 vaeyavemph ssvggshled asgeihppkl vlkkinskrs lkqpleqnqt isplstyees 421 kvskyafelv dkqalldseg nadidqvdnl qegpskpvhs stnyddamqf lkkkrylqaa 481 snnsreyaln vgtirsqpsv tqaavasvid esttasiles qalnveiksn hdknvipdev 541 lqtlldhysh kangqheisf svadtevtss isinssevpe vtpsenvgss sqasssdkan 601 mlqeyskflq qaldrtsqnd aylnspslnf vtdnqtlpnq pafssidkqv yatmpinsfr 661 sgmnsplrtt pdkshfgliv gdsqhsfpfs gdetnhasat stqdfldqvt sqkkaeaqpv 721 hqayqmssfe qpfrapyhgs ragiatqfst angqvnlrgp gtsaefsefp lvnvndnrag 781 mtsspdattg qtfg
Specificity This polyclonal antibody is specific for ZBP-89.
Purification Delipidation and defibrination
Alternative Name ZNF148 (ZNF148 Antibody Abstract)
Background The GI tract abundantly expresses growth factors many of which bind and activate the EGF receptor present on mucosal cells. One such factor is the zinc finger protein (ZBP-89) that binds to a GC-rich DNA element in the gastrin promoter and confers EGF responsiveness. The fulllength protein functions as a repressor of growth factor signals regulating the gastrin promoter. Several other growth related promoters are also regulated by ZBP-89. ZBP-89 is one of a family of related transcriptional regulators. It has been reported in recent studies that ZBP-89 regulates growth in part by stimulating the cyclin-dependent kinase inhibitor, p21waf1, in a butyrate-dependent manner through recruitment of the histone acetyl transferase p300. Moreover, ZBP-89 triggers growth arrest in a p53-dependent manner by preventing nuclear export of p53. ZBP-89 also induces apoptosis, but this process occurs independent of p53.Synonyms: Transcription factor ZBP-89, ZBP89, Zinc finger DNA-binding protein 89, Zinc finger protein 148
Gene ID 7707
NCBI Accession NP_068799
UniProt Q9UQR1
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Application Notes This polyclonal antibody reacts human ZBP-89 in a variety of immunological assaysincluding western blot and ELISA. This antibody is likely functional inimmunohistochemistry and immunoprecipitation. Recommended dilutionsImmunoblotting: 1: 5,000. A band at approximately 89 kDa corresponding to human ZBP-89is detected. Human monocytes or macrophages or nuclear extracts from PMA treated U937cells can be used as a positive control. ELISA: 1: 10,000 - 1: 30,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 90 mg/mL (by Refractometry)
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
Supplier Images
 image for anti-Zinc Finger Protein 148 (ZNF148) antibody (ABIN117932) anti-Zinc Finger Protein 148 (ZNF148) antibody
 image for anti-Zinc Finger Protein 148 (ZNF148) antibody (ABIN117932) anti-Zinc Finger Protein 148 (ZNF148) antibody (Image 2)
 image for anti-Zinc Finger Protein 148 (ZNF148) antibody (ABIN117932) anti-Zinc Finger Protein 148 (ZNF148) antibody (Image 3)
Background publications Bai, Yoon, King, Merchant: "ZBP-89-induced apoptosis is p53-independent and requires JNK." in: Cell death and differentiation, Vol. 11, Issue 6, pp. 663-73, 2004 (PubMed).

Bai, Merchant: "Transcription factor ZBP-89 is required for STAT1 constitutive expression." in: Nucleic acids research, Vol. 31, Issue 24, pp. 7264-70, 2003 (PubMed).

Chen, Merchant, Lai, Ho, Hu, Okada, Huang, Chui, Law, Li, Lau, Li: "Mutation of p53 in recurrent hepatocellular carcinoma and its association with the expression of ZBP-89." in: The American journal of pathology, Vol. 162, Issue 6, pp. 1823-9, 2003 (PubMed).

Zhang, Diab, Zehner: "ZBP-89 represses vimentin gene transcription by interacting with the transcriptional activator, Sp1." in: Nucleic acids research, Vol. 31, Issue 11, pp. 2900-14, 2003 (PubMed).

Park, Shelley, Arnaout: "The zinc finger transcription factor ZBP-89 is a repressor of the human beta 2-integrin CD11b gene." in: Blood, Vol. 101, Issue 3, pp. 894-902, 2003 (PubMed).

Bai, Merchant: "ZBP-89 promotes growth arrest through stabilization of p53." in: Molecular and cellular biology, Vol. 21, Issue 14, pp. 4670-83, 2001 (PubMed).

Dawson, Park, Chen, Chao, Dousman, Waleh, Hobbs, Jong, Toll, Zhang, Gu, Agadir, Merchant, Bai, Verma, Thacher, Chandraratna, Shroot, Hill: "Retinoic acid (RA) receptor transcriptional activation correlates with inhibition of 12-O-tetradecanoylphorbol-13-acetate-induced ornithine decarboxylase (ODC) activity by retinoids: a potential role for trans-RA-induced ZBP-89 in ODC inhibition." in: International journal of cancer. Journal international du cancer, Vol. 91, Issue 1, pp. 8-21, 2001 (PubMed).

Keates, Keates, Kwon, Arseneau, Law, Bai, Merchant, Wang, Kelly: "ZBP-89, Sp1, and nuclear factor-kappa B regulate epithelial neutrophil-activating peptide-78 gene expression in Caco-2 human colonic epithelial cells." in: The Journal of biological chemistry, Vol. 276, Issue 47, pp. 43713-22, 2001 (PubMed).

Bai, Merchant: "Transcription factor ZBP-89 cooperates with histone acetyltransferase p300 during butyrate activation of p21waf1 transcription in human cells." in: The Journal of biological chemistry, Vol. 275, Issue 39, pp. 30725-33, 2000 (PubMed).

Wieczorek, Lin, Perkins, Law, Merchant, Zehner: "The zinc finger repressor, ZBP-89, binds to the silencer element of the human vimentin gene and complexes with the transcriptional activator, Sp1." in: The Journal of biological chemistry, Vol. 275, Issue 17, pp. 12879-88, 2000 (PubMed).

Taniuchi, Mortensen, Ferguson, Greenson, Merchant: "Overexpression of ZBP-89, a zinc finger DNA binding protein, in gastric cancer." in: Biochemical and biophysical research communications, Vol. 233, Issue 1, pp. 154-60, 1997 (PubMed).