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SET Domain, Bifurcated 1 (SETDB1) (AA 1058-1075) antibody

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Synonyms SETDB1, NV10069, fc07d03, si:ch211-81a5.6, wu:fc07d03, ESET, H3-K9-HMTase4, KG1T, KMT1E, TDRD21, AU022152, mKIAA0067
AA 1058-1075
(20), (8), (5), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1)
(93), (34), (20), (10)
(69), (24)
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Enzyme Immunoassay (EIA), Western Blotting (WB)
(78), (45), (20), (11), (9), (9), (7), (4), (3), (3), (1)
Pubmed 4 references available
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Quantity 0.1 mg
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1058-1075 of human ESET.
Sequence Human ESET, 1291 aa, predicted MW 143.2 kDa: 1 msslpgcigl daatatvese eiaelqqavv eelgismeel rhfideelek mdcvqqrkkq 61 laeletwviq kesevahvdq fddasravt nceslvkdfy sklglqyrds ssedessrpt 121 eiieipdedd dvlsidsgda srtpkdqkl reamaalrks aqdvqkfmda vnkksssqdl 181 hkgtlsqmsg elskdgdliv smrilgkkrt ktwhkgtlia iqtvgpgkky kvkfdnkgks 241 llsgnhiayd yhppadklyv gsrvvakykd gnqvwlyagi vaetpnvknk lrfliffddg 301 yasyvtqsel ypicrplkkt wediediscr dfieeyvtay pnrpmvllks gqlikteweg 361 twwksrveev dgslvrilfl ddkrcewiyr gstrlepmfs mktssasale kkqgqlrtrp 421 mgavrskgp vvqytqdltg tgtqfkpvep pqptappapp fppapplspq agdsdlesql 481 qsrkqvakk stsfrpgsvg sghssptspa lsenvsggkp ginqtyrspl gstasapaps 541 alpappappv fhgmlerapa epsyrapmek lfylphvcsy tclsrvrpmr neqyrgknpl 601 lvpllydfrr mtarrrvnrk mgfhviyktp cglclrtmqe ierylfetgc dflflemfcl 661 dpyvlvdrkf qpykpfyyil ditygkedvp lscvneidtt pppqvayske ripgkgvfin 721 tgpeflvgcd ckdgcrdksk cachqltiqa tactpggqin pnsgyqykrl eeclptgvye 781 cnkrckcdpn mctnrlvqhg lqvrlqlfkt qnkgwgircl ddiakgsfvc iyagkiltdd 841 fadkeglemg deyfanldhi esvenfkegy esdapcssds sgvdlkdqed gnsgtedpee 901 snddssddnf ckdedfstss vwrsyatrrq trgqkengls ettskdshpp dlgpphipvp 961 psipvggcnp psseetpknk vaswlscnsv seggfadsds hssfktnegg egraggsrme 1021 aekastsglg ikdegdikqa kkedtddrnk msvvtessrn ygynpspvkp eglrrppskt 1081 smhqsrrlma saqsnpddvl tlsssteseg esgtsrkpta gqtsatavds ddiqtissgs 1141 egddfedkkn mtgpmkrqva vkstrgfalk sthgiaikst nmasvdkges apvrkntrqf 1201 dgeescyii daklegnlgr ylnhscspnl fvqnvfvdth dlrfpwvaff askriragte 1261 ltwdynyevg svegkellcc cgaiecrgrl l
Specificity This is an affinity purified antibody produced by Immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human ESET protein. Cross reactivity with ESET protein from non-human sources is not expected based on a lack of homology (BLAST) with other known proteins.
Purification Immunoaffinity chromatography.
Alternative Name SETDB1 (SETDB1 Antibody Abstract)
Background The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities. ESET functions as a histone methyltransferase by methylation of Lys-9 of histone H3. H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. ESET shows a nuclear localization and is associated with non-pericentromeric regions of chromatin, and is excluded from nucleoli and islands of condensed chromatin. Although ESET is a widely expressed protein, it is highly expressed in the testis.Synonyms: ERG-associated protein with SET domain, ESET, H3-K9-HMTase 4, Histone H3-K9 methyltransferase 4, Histone-lysine N-methyltransferase SETDB1, KIAA0067, KMT1E, Lysine N-methyltransferase 1E, SET domain bifurcated 1
Gene ID 9869
NCBI Accession NP_001138887
UniProt Q15047
Application Notes This affinity purified antibody has been tested for use in ELISA (1: 10,000-1: 50,000) andWestern blotting (1: 1,000-1: 2,500). Western blotting shows reactivity specific for human ESET detecting a band atapproximately 170 kDa. Specific band detection by Western blot is blocked by peptidecompetition by pre-incubating the antibody with the immunizing peptide prior to reactionwith the membrane. Reactivity in other immunoassays is unknown.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.17 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-SET Domain, Bifurcated 1 (SETDB1) (AA 1058-1075) antibody (ABIN117938) Western blot analysis is shown using anti-ESET antibody to detect human ESET present ...
Background publications Sarraf, Stancheva: "Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly." in: Molecular cell, Vol. 15, Issue 4, pp. 595-605, 2004 (PubMed).

Wang, An, Cao et al.: "mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression." in: Molecular cell, Vol. 12, Issue 2, pp. 475-87, 2003 (PubMed).

Schultz, Ayyanathan, Negorev et al.: "SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins." in: Genes & development, Vol. 16, Issue 8, pp. 919-32, 2002 (PubMed).

Harte, Wu, Carrasquillo et al.: "Assignment of a novel bifurcated SET domain gene, SETDB1, to human chromosome band 1q21 by in situ hybridization and radiation hybrids." in: Cytogenetics and cell genetics, Vol. 84, Issue 1-2, pp. 83-6, 1999 (PubMed).

Catalog No. ABIN117938
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